臺灣博碩士論文加值系統

本文探討靛藍法測氨中各試劑之干擾問題，並設法改進之。測定海水或是半鹹水時，最後pH不能恆定，乃肇因於一未知的酸鹼緩衝系統，此系統應為檸檬酸試劑與樣水中鎂離子形成之離子對。此系統會造成 pH 漂移，使吸光係數降低，且干擾濃度標定。經 pH 修正後可使海淡水吸光係數為一定值( 21000/M/cm )。另發現次氯酸試劑所產生之氯氣會和酚試劑作用出與 indophenol blue 吸收光譜相近之產物，是一項新發現干擾來源。由本文建議之操作步驟將可使靛藍法測氨氮更為準確

This study focuses on the pH and buffering capacity problems involved in the determination of ammonia in seawater by the indophenol blue colo-rimetric method. The pH-shift puzzle was unraveled by acid-base titration. The major buffering capacity for the indophenol blue method is induced by the combination of the phenate system (pK = 10) and a previous — un-known Mg-citrate system. By adding extra alkali reagent to compensate for the pH difference in saline media, the molar absorptivity was found to be 21000 /M/cm at 640 nm. For sample with a variety of salinities, an empirical equation is suggested to correct the results.

目錄
目錄I
圖目錄III
表目錄IV
摘要V
第一章 前言1
1.1 概述1
1.2 靛藍法之沿革與原理1
1.3 研究動機5
第二章 PH與緩衝能力對於靛藍法的影響7
2.1 概述7
2.2 PH對於靛藍法的影響7
2.2.1 試劑及方法7
2.2.2 結果與討論8
2.3 緩衝系統對PH漂移的影響9
2.3.1 試劑與方法9
2.3.2 緩衝能力的定義及計算方式11
2.3.3 結果與討論11
第三章 靛藍法的試劑穩定程度及污染影響21
3.1 概述21
3.2 漂白水及DIC試劑中有效氯之穩定性21
3.2.1 簡介21
3.2.2 次氯酸定量之方法21
3.2.3 試劑與方法22
3.2.4 操作步驟22
3.2.5 結果及討論23
3.3 空氣污染及容器材質對靛藍法之干擾23
3.3.1 簡介23
3.3.2 試劑及方法23
3.3.3 結果與討論24
第四章 靛藍法中試劑混和後產生藍色干擾物之探討28
4.1 新發現之干擾來源28
4.1.1 藍色干擾物之發現28
4.1.2 試劑與方法29
4.1.3 結果與討論30
4.2 DIC中溶解氣體的濃度和神秘藍色的關係31
4.2.1 簡介31
4.2.2 試劑及方法31
4.2.3 結果與討論32
4.3 神秘藍色對於靛藍呈色的干擾32
4.3.1 簡介32
4.3.2 操作步驟33
4.3.3 討論與結果33
第五章 本文建議方法45
5.1 避免干擾來源45
5.2 建議配方46
5.3 方案(A)淡水樣品之操作步驟46
5.3.1 計算方式46
5.4 方案(B)海水樣品之操作步驟47
5.4.1 計算方式47
5.5 方案(C)半鹹水樣品之操作步驟48
5.5.1 計算方式48
第六章 應用靛藍法於感潮帶氨氮檢測51
6.1 概述51
6.2 淡水河口之採樣點敘述51
6.3 試劑及方法51
6.4 感潮帶鹽度與校正後氨氮之關係52
第七章 結論60
參考文獻61

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