臺灣博碩士論文加值系統

中文摘要
樟芝是一種台灣特有種的真菌類，近年來樟芝被宣稱有良好的抗癌及抗氧化效果而聲名大噪。但是野生的樟芝只寄生在台灣特有的牛樟樹上，不但數量少之又少，而且品質不穩定。由於科學的進步，近年來樟芝已被利用發酵培養企圖達到
大量生產的目的。但是在發酵培養樟芝時，建立一個適當的生物活性依據是良好發酵操作的前提。
本實驗嘗試利用動物細胞Hepa 1c1c7中的Quinone reductase (QR)來作為樟芝生物活性的依據。探討在不同的發酵時間以及不同的發酵條件下，樟芝發酵液對Hepa 1c1c7細胞中的QR的誘導活性。
實驗結果發現，樟芝的後期發酵液較前期更能有效地抑制Hepa 1c1c7細胞的增生，以及誘導Hepa 1c1c7細胞中的QR表現。Hepa 1c1c7細胞中的QR誘導活性會隨著所添加的發酵液之發酵時間而先增後減，而且其趨勢與樟芝發酵液中的總酚含量之趨勢非常類似。故推測兩者之間應該有很大的關聯性。再者，通氣量越小的發酵液，對Hepa 1c1c7的QR誘導活性越高。此趨勢和通氣量對具有良好抗氧化性的多醣產量的影響相反。
由本實驗的結果推定，利用抗癌酵素QR作為樟芝發酵時生物活性的依據，應該是個可行的方式。

Abstract
Antrodia camphorata is a particular fungus in Taiwan. Many papers have showed that it is a good source of anti-oxidant and anti-cancer ability in recent years. Wild Antrodia camphorata parasitizes specifically on Cinnamomum kanehirai hays in a small quantity and its quality is not stable.
Recent years, Antrodia camphorata was tried to be produced on large scale by submerged culture. Thus, building up a bioactivity index for Antrodia camphorata in submerged culture is important.
In this study, quinone reductase (QR) in Hepa 1c1c7 cells were used to measure the bioactivity of Antrodia camphorata in submerged culture under different conditions.
It was found that the fermentation broth in the late fermentation period is more powerful to induce QR in Hepa 1c1c7 cells. QR in Hepa 1c1c7 cells was found to increase with the fermentation time initially and then it reduced. The tendency of QR induction was similar to that of total phenolic compounds in the fermentation broth and was thought to be related. It was also found that the fermentation broth at a less aeration rate can stronger induce QR specific activity than that at a larger aeration rate. This tendency is reversed to that of the polysaccharides quantity in fermentation broth.
From the results it was concluded that QR may be a workable index for Antrodia camphorata in submerged culture.

Table of content

Abbreviations----------------------------------------------------------------------1
Abstract-----------------------------------------------------------------------------2
Introduction------------------------------------------------------------------------5
Materials and Methods----------------------------------------------------------22
Results-----------------------------------------------------------------------------38
Conclusions-----------------------------------------------------------------------50
References------------------------------------------------------------------------53
Appendices-----------------------------------------------------------------------61

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