臺灣博碩士論文加值系統

在過去數十年中，生物分子和表面的交互作用一直以來都是研究學者所注意的領域之一，這些作用統稱為生物介面科學。在生物介面科學的發展之下，連帶探討出許多生物分子的特性以及對於生物環境的交互作用，而且其應用在許多人工材料上發展出許多新興領域和科技，例如:生物醫學、組織工程、微陣列分析系統、生物感測元件等。為了有效發展生物介面科學，對於生物分子鍵結是一大重點之一，而化學共價鍵結生物分子因為其持久性以及穩定性的優點，加上生物正交性(bio-orthoogonal)的生物耦合鍵結特性，能快速且準確應用於各種領域中。
由於許多表面上無具有官能基，例如金屬、陶瓷等，將生物分子固定在其上是一大困難，因此研究對於各種材料的表面改質是一大需要克服問題。而在文獻指出，化學氣相沉積具有官能性的聚對二甲苯，只需一步驟即可簡易地達到表面改質，且化學氣相沉積改質各種材料的選擇性極低，而為了因應生物分子的鍵結，發展出不同種類官能基的聚對二甲苯高分子來應用到生物領域上，例如具有炔基、胺基等官能性之聚對二甲苯等。
本研究就是以多樣化具有官能性的對二甲苯二聚體，以氣相沉積共聚法製備官能性的聚對二甲苯薄膜用來改質人工材料，再利用各種不同特殊性的生物耦合技術來達到控制生物分子鍵結，並且應用在許多生物領域上，例如控制細胞生長、抗汙表面等。除此之外，若能控制化學氣相沉積技術相信對於生物領域上的研究會更有助力，在本研究中嘗試以電流和化學氣相沉積流速來控制聚對二甲苯生長，達到選擇性地化學氣相沉積於人工材料上。

In past decades, the field of biointerface science, which is the reaction between surface and biomolecules, has been focused by chemists and biologists. Because more and more papers reported about surface biological chemistry, the functions of biomolecules and the interactions between biomolecules and environments has been discussed, and they were applied in various types of artificial materials. Moreover, according to growth of biointerface science, many new fields and technologies have been developed, such as biomedical, tissue engineering, micro total analysis systems (μTAS) ans biosensors. For the studying surface biological chemistry effectively, the selective conjugation onto surface is one of most important issue and, for the characteristics of enduing and stability, the covalence conjugation is superior. For the reason of the characteristics, the recently developing surface bioconjugations are bio-orthoogonal, fast, and site-specific, including the carbondimide reaction of amine and carboxylic acid, the thiol-ene reacton and the click chemistry. However, many artificial materials have no functional group such as metals and ceramics. It is the reason that a new field of surface modification has raised. Through chemistry vapor deposition, coating functional poly-p-xylene onto surface to modify surface is a one-step process, and it doesn’t have any inhibition for materials. Even, for the functional groups of biomolecules, papers dicected that various types of functional poly-p-xylene, such as Poly (4-ethynyl-p-xylylene-co-p-xylylene), Poly (4- aminomethyl-p-xylylene-co-p-xylylene), have been synthesized to conjugate the biomolecules and apply in many fields.
My research is that synthesize various functional [2,2] paracyclophane and use them to modify surface through chemistry vapor deposition. For the unique selective bioconjugtions, it can control the covalence of biomolecules and can be applied in biology fields such as controlling cell growth and anti-fouling. Moreover, we developed a new technology to control the chemical vapor deposition. Electric and deposition rate can control the deposition of functional poly-p-xylene onto specific area to achieve selective chemistry vapor deposition. For the new technology, surface modification can be precise, low limits, and applied extensively.

誌謝……………………………………………………………………........i
中文摘要……………………………………………………………….......ii
Abstract……………………………………………………………………iii
英文縮寫說明…………………………………………………………….IV
第一章 緒論……………………………………………………………………………………………………………1
1.1生物介面科學…………………………….……..………………...1
1.2生物介面耦合技術介紹…………………………………………...1
1.3 化學氣相沉積系統應用於生物耦合技術……………….………6
1.4 研究動機………………………………………..…..…………….7
第二章 實驗………………………………………………………...……8
2.1試片及材料之製備…………………………………………....…...8
2.2合成官能性對二甲苯二聚體………………………………….....10
2.3化學氣相沉積共聚法製備官能性聚對二甲苯……………….…17
2.3.1單向化學氣相沉積共聚單一官能性聚對二甲苯………..18
2.3.2雙向化學氣相沉積共聚多元官能性聚對二甲苯………...23
2.4官能性聚對二甲苯應用於表面生物耦合技術………………….24
2.4.1單一官能性聚對二甲苯應用於表面生物耦合技術……...24
2.4.2多元官能性聚對二甲苯應用於表面生物耦合技術……...32
2.5蛋白質吸附試驗………………………………………….………34
2.6細胞培養試驗…………………………………………….………34
2.7電流對官能性聚對二甲苯沉積試驗……………………...……..35
2.8沉積流速對官能性聚對二甲苯沉積試驗…………………...…..35
2.9實驗設備及分析儀器………………………………………...…35
第三章 實驗結果與討論………………………………………………………………………38
3.1 表面特性分析……….…………………………………………..39
3.2 化學氣相沉積共聚法製備官能性聚對二甲苯鍍膜…………...45
3.2.1單一官能性聚對二甲苯鍍膜……………………...………45
3.2.2多元官能性聚對二甲苯鍍膜……………………………...50
3.3官能性聚對二甲苯於表面生物耦合技術之應用……...………..52
3.3.1單一官能性聚對二甲苯於表面生物耦合技術之應用…...52
3.3.2多元官能性聚對二甲苯於表面生物耦合技術之應用…...68
3.4電流對化學氣相沉積選擇性影響……………………………….70
第四章 結論………………………………………………..…………….75
第五章 未來展望…………………...…………………………………....76
5.1生物耦合技術…………………………………………..………...76
5.2多官能性高分子與表面梯度組成………………………….....…76
5.3具有選擇性的化學氣相沉積效果…....………………..……...…77
參考文獻……………………………………………………………...…..78
附錄…………………………………………………………………….....85

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