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DNA指紋具孟德爾遺傳特性，在鑑定生物之遺傳變異上為一極新而有力之
工具。 本試驗之目的以重複序列寡核酸做為探針，進行荷蘭種乳牛、
台灣水牛及豬之DNA指紋分析，供個體鑑別、系譜分析、品種鑑定、性別
鑑定或與經濟性狀有關連性等之探討。牛與豬之基因組DNA經限制HinfI
或HaeⅢ切割後， 進行瓊脂糖 之將膠體乾燥，與放射線標定之重複序列
探針(TG)~bs1;6~bs0;進行膠體原位雜交，最後膠片覆以X-光片，使之自
動放射顯影，產生清晰的DNA指紋。試驗結果證明重複序列探針可用於牛
與豬之DNA指紋分析。 限制不同、電泳條件不同，產生的DNA指紋有極
大的差異。與HinfI比較，限制HaeⅢ切割後，需較長的電泳時間以分開
DNA片段，產生的環帶數亦較多。分別計算牛與豬之共有環帶頻率， 可供
分析DNA指紋資料之用。結果顯示，不同品種動物間之遺傳變異大於同種
動物，同種動物之DNA指紋有較高之相似性。探針(TG)~bs1;6~bs0;偵測則
缺乏品種特異性。以HaeⅢ切割不同性別荷蘭種乳牛之基因組 DNA，雜交
後之DNA指紋中，有一長度約15Kb之公牛特有環帶存在。 此DNA片段回收
後，用限制HindⅢ或EcoRI割切後， 接入載體 pUC18以篩選重組質體，
得到9個重組質體，長度在0.3-1Kb間。為了解其序列組成，以雙去氧法進
行序列分析。其中7個重組質體之部分序列巳定序清楚。 這些重組質體是
否有性別特異序列或重複序列存在，供性別鑑定探針之用，有待進一步探
討。
Genomic DNA was digested with restriction endonuclease HinfI
or HaeⅢ and hybridized with probe (TG)~bs1;6~bs0;.
fingerprints were obtained in these traits. The results
showed that the simple tadem repeat probe may be suitable
for the DNA fingerprinting in cattle and pig. The band-
sharing probability were also estimated for related and
unrelated animals. Results showed that individuals within
breeds tented to be more similar to each other with regard to
DNA fingerprint pattern than to individuals of other breeds.
The DNA fingerprints of Holstein cattle showed a 15 kb
sex-specific band presented in the male only when genomic DNA
was digested with HaeⅢ and hybridized with~bs0;. The male-
specific DNA fragement was recovered from an agarose gel and
digested with HindⅢ or EcoRI,then ligated into pUC18.Nine
clones had been screened,these inserted fragments were 0.3-1
Sequencing of these clones have been done excepted 2 There
are two clones showing highly homology.These clones can
be used as probe for sex determination or not need to be
investigated in the future.
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