臺灣博碩士論文加值系統

流行病學研究指出，關於糖尿病血糖平衡有兩大指標，一為空腹血糖，另外一個為飯後血糖，其中控制飯後血糖更為重要，α-葡萄糖苷酶抑制劑具有有效降低飯後血糖之效果，但服用α-葡萄糖苷酶抑制劑降血糖藥後常會發生腸胃不適的症狀，除此更有研究指出合成α-葡萄糖苷酶抑制劑可能會增加腎臟腫瘤、嚴重肝損傷及急性肝炎的發生率。牛樟芝具有許多的有效成分具有抗肝癌、血癌與胰臟癌增加免疫能力、抗過敏、降血壓、降血糖的效果經由本研究結果指出牛樟芝發酵液以及牛樟芝菌絲體抑制α-葡萄糖苷酶活性的IC50分別為318以及380mg/ml的抑制效果，發酵液的抑制效果高於菌絲體，可以作為α-葡萄糖苷酶抑制劑。

Epidemiological studies indicate that diabetes two indicators on blood sugar balance , one is fasting glucose, another is postprandial glucose level.
Postprandial glucose level is more important to control blood sugar after a meal, when α-glucosidase inhibitors would reduce its effect.
Taking the α-glucosidase inhibitors to manage blood glucose level result in hypoglycemic symptoms of gastrointestinal discomfort,and many studies have pointed out the synthestic of α-glucosidase inhibitors may would increase kidney tumors, severe liver injury and the occurrence of acute hepatitis rate. Antrodia was known of many active ingredients with anti-cancer, leukemia and pancreatic cancer, increased immunity, allergy, and blood pressure, and blood glucose lowering effect. According to the results of this study indicate Antrodia broth and mycelium of Antrodia show respectively 318 and 380 mg/ml inhibitory effect. On α-glucosidase activity and the inhibitory effect of the fermented liquid is how even higher than that of the mycelium, compared to other naturak herbs.excessive doses needed who found in Antroidia, it is less suitable an excellent α-glucosidase inhibitor than connent found onto.

目錄:
頁次
致謝……………………………...……………………………………………………X
目錄………………………………………………………………………………...1
摘要………………………………………………………………………………...3
(一)中文摘要……………………………………………………………………….3
(二)英文摘要……………………………………………………………………….4
圖目錄………………………………………………………………………………5
表目錄……………………………………………………………………………....7
壹:文獻回顧………………………………………………………………………...8
(一)牛樟芝簡介…………………………………………………………………….8
1.三萜類化合物……………………………………………………………………..9
2.多醣體…………………….………………………………………………………9
3.超氧岐化酶……………………………………………………………………….10
4.核酸類…………………………………………………………………………….12
(二)糖尿病簡介……………………………………………………………………13
(三)α-葡萄糖苷酶(α-Glucosidase)………………………………………………...14
(四)α-葡萄糖苷酶抑制劑(α-Glucosidase inhibitor)……………….………………16
貳:材料與方法………………………………………………………………………21
一、實驗材料及藥品……………………………………………………………….21
(一)實驗藥品………………………………………………………………………..21
(二)實驗器材………………………………………………………………………..22
二.實驗方法……………………………………………...………………………….23
(一)牛樟芝的培養……………………………………………………………….23
(1)牛樟芝的平板培養…………………………………………………...………….24
(2)牛樟芝液態培養…………………………………………………………...…….25
(3)牛樟芝菌絲體液態的培養…………………………………………………..…25
(二)樣品萃取……………………………………….……………………….….34
(1)樣品取得…………………………………………………….………..…34
(2)索氏萃取法…………………………………………………………….34
(3)水浴加熱法………………………………………………………...34
(三)酵素抑制活性測定…………………….…………..……………….35
(1)酵素製備……………………………………………………..35
(2)活性分析……………………………………………………...35
(3)抑制率計算…………………………………………………....35
(四)HPLC原理:……………………………………….…………………………..36
(1)超濾質譜技術…………………………………….……………………… ….36
(2)HPLC的使用條件………………………………………………………………...38
(3)HPLC的操作步驟…………..……………………….……………………….….38
參:結果與討論………………………………………….………………………..…39
(一)萃取回收率的探討…….…....……………………………..……………….39
(二)不同萃取方式以及不同濃度牛樟芝萃取物對於α-葡萄糖苷酶IC50的比較...41
(三)超過濾液相層析儀法篩選抑制劑之可行性探討.............................................57
肆:結論......................................................................................................................59
伍:參考文獻.................................................................................................................60

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