臺灣博碩士論文加值系統

視叉上核 (suprachiasmatic nucleus, SCN)是哺乳類動物體內的主要生理節律器，從解剖學與功能上，SCN可分為背側SCN(dorsal SCN, dSCN) 與腹側SCN(ventral SCN, vSCN)。先前研究指出，SCN細胞上有尼古丁型乙醯膽鹼接受器(Nicotinic acetylcholine receptor, nAChR)及蕈毒型乙醯膽鹼接受器(Muscarinic acetylcholine receptor, mAChR)，且乙醯膽鹼可以直接作用及影響SCN細胞。本論文利用細胞外紀錄的方法(cell-attached recording technique)想進一步探討膽鹼類物質對SCN細胞自發性動作電位(spontaneous firing rate, SFR)的影響且著重於時間性相關研究。
實驗結果中得知，膽鹼類物質carbachol(CCh)、nicotine(Nict)與muscarine(Musc)會影響SCN細胞的SFR，所造成的SFR反應比率分佈，具有相位依賴性(phase–dependent)。非專一性AChR致效劑CCh對SFR的作用主要透過mAChR，而dSCN細胞可能主要由M1-mAChR所調控。dSCN和vSCN細胞對膽鹼類物質的SFR影響基本上一致，但在early night (N1：CT 12-17, 1900- 0100)與高低濃度間則有所不同。以上結果得知膽鹼類物質對SCN細胞的影響在生理上可能扮演重要角色˙

The suprachiasmatic nucleus (SCN) is the master pacemaker in mammals, with two anatomically and functionally distinct divisions of dorsal (dSCN) and ventral SCN (vSCN). In the SCN, both muscarinc and nictonic cholinergic receptors have been shown to be present and acetylcholine acts directly on SCN neurons.
In this study, I used the cell-attached recording technique to investigate the effects of cholinergic agents on the SCN neurons, focusing on the time-dependent responses of both dSCN and vSCN neurons. I found that cholinergic agents altered the spontaneous firing rate (SFR) and the effects exhibited a circadian rhythm. Comparing the response profiles of muscarine (Musc) and nicotine (Nict) to that of carbachol (CCh) indicated that the carbachol responses were most likely mediated by the mAChR such as M1-mAChR on the dSCN neurons. The cholinergic responses of dSCN and vSCN neurons were in a similar way. However, the muscarinic responses of dSCN and vSCN neurons differed during the early night and during the low and high concentration. These data suggested that cholinergic effects on the neurons of the SCN might play an important role.

致謝……………………………………………………………………….i
中文摘要…………………………………………………………………ii
英文摘要………………………………………………………………...iii
目錄……………………………………………………………………...iv
圖次……………………………………………………………………….v
第一章 序論……………………………………………………………..1
一、 視叉上核 (suprachiasmatic neucleus, SCN)…………………2
二、 膽鹼系統 (cholinergic system)……………………………….6
三、 SCN與膽鹼系統的相互關係……………………………….15
第二章 實驗材料與方法………………………………………………19
第三章 實驗結果………………………………………………………23
第四章 討論……………………………………………………………35
圖表及說明……………………………………………………………..41
參考文獻………………………………………………………………..64

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