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研究生:王銘鴻
研究生(外文):Wang, Ming- Hung
論文名稱:葉面噴施細胞分裂素與硝酸鈣對文心蘭生長與花序發育及品質之影響
論文名稱(外文):Effects of Foliar Spray with Cytokinin and Calcium Nitrate on Growth and Inflorescence Development and Quality of Oncidiums Honey Angel.
指導教授:顏昌瑞
指導教授(外文):Yen, Chung - Ruey
口試日期:2015-05-05
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2015
畢業學年度:103
語文別:中文
論文頁數:65
中文關鍵詞:文心蘭產期調節細胞分裂素
外文關鍵詞:OncidiumCytokininRegulation of FloweringCalcium
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摘要
學號:N10211009
論文名稱:葉面噴施細胞分裂素與硝酸鈣對文
心蘭生長與花序發育及品質之影響 總頁數:65
學校名稱:國立屏東科技大學 系(所)別:農園生產系
畢業時間及摘要別:103學年度第二學期碩士學位論文摘要
研究生:王 銘 鴻 指導教授:顏 昌 瑞
論文摘要內容:
本試驗以目前臺灣文心蘭主要切花品種 Oncidium ‘Honey Angel’ (俗稱‘檸檬綠’)為試驗材料,探討養液栽培配合葉面噴施 Kinetin、6-benzyladeninepurine (BA) 及硝酸鈣(Ca(NO3)2) 對文心蘭花序發育及品質、營養芽生長之影響,以期調節文心蘭在非盛產期開花及提高花序品質。
植株於2014年6月中旬營養芽萌發,9月假球莖出鞘,文心蘭 Oncidium ‘Honey Angel’ 植株生長期間因經歷臺灣屏東炎熱夏季,溫度30oC 以上高溫,假球莖瘦扁、碳水化合物蓄積不足,抽梗後葉面噴施細胞分裂素( Cytokinin, CTKs ),並無法改善花梗生長與切花品質,與對照組無顯著差異。但是對營養芽數增加與縮短當代假球莖營養芽萌發天數,則有顯著差異,文心蘭 ‘Honey Angel’ 葉面噴施 Kinetin (150、200 ppm )較對照組提早10天萌發營養芽;而葉面噴施0.6、0.8 mM (BA) 營養芽數為1.9芽數、營養芽萌發提早在切花前5.8天萌發,較對照組提
早27天萌發營養芽,皆較對照組有顯著差異。抽梗後葉面噴施硝酸鈣 Ca(NO3)2 對文心蘭 Oncidium ‘Honey Angel’ 植株生長與切花品質則有些微提升作用,以300 ppm 硝酸鈣Ca(NO3)2 處理,假球莖寬、厚度及切花品質(花梗長、分叉數及小花朵數)較對照組有顯著提升,但營養芽數與萌發營養天數,則與對照組無顯著差異。
關鍵字:文心蘭、產期調節、細胞分裂素、鈣

Abstract
Student ID:N10211009
Title of thesis:Effects of Foliar Spray with Cytokinin and Calcium Nitrate on Growth and Inflorescence Development and Quality of Oncidiums Honey Angel.
Total page:65
Name of Institute:Department of Plant Industry
Graduate date:2015.5 Degree Conferred:Master
Name of student:Ming- Hung Wang Adviser:Chung - Ruey Yen Ph.D.
The contents of abstract in this thesis:

Effects of foliar spray cytokinins (kinetin and BA) and calcium on growth and flowering in Oncidium Onc. “Honey Angel” were studied to promote off-season and improve inflorescences quality. The applications of cytokinins in warm Pingtung summer did not improve flower growth and quality. However, the treatments significantly increased vegetative bud number and shortened the days of pseudobulbs bud emergence.
Foliar kinetin (150-200ppm) spray promoted vegetative bud emergence 9 days earlier than control. The growth of vegetative buds (1.9) of 0.6-0.8Mm 6-benzyladeninepurine (BA) was 5.8 days before cut flower, and 27 days earlier than control. The treatment of 300ppm Ca(NO3)2 increased plant growth and flower quality. The width and thickness of the pseudobulbs and
length of flower stalks and number of branch and florets were significantly better than control. However, the number of vegetative buds and the days of bud emergence were not different.
Key words:Oncidium、Regulation of Flowering、Cytokinin、Calcium

目錄
中文摘要………………………………………………………………………I
Abstract...……………….…………………………………………...III
致謝…………………………………………………………………………..V
目錄………………………………………………………………………….VI
表目錄……………………………………………………………………….Ⅷ
圖目錄……………………………………………………………………….Ⅹ
前言…………………………………………………………………………...1
前人研究
ㄧ、文心蘭生育特性………………………………………………………3
(一)原產地及分類.……………………………………………………...3
(二)生育習性.…………………………………………………………...3
(三)生育階段.…………………………………………………………...4
二、假球莖與生長之關係…………………………………………………5
三、溫度對文心蘭生長與開花之影響……………………………………6
四、光強度對文心蘭生長與開花之影響…………………………………7
五、細胞分裂素(cytokinins )對植物生育之影響…………….…………..8
(一) kinetin與植物生長關係………………………………….………...9
(二) 6-benzyladeninepurine ( BA ) 與植物生長的關係…………........10
六、鈣肥於蘭科植物之運用…………………………………………….11
材料與方法
ㄧ、植物材料與管理…………………………………………………….14
二、試驗方法…………………………………………………………….14
試驗一 抽梗後噴施不同濃度 Kinetin 對文心蘭生長與開花
的影響……………………………………………………...14
試驗二 抽梗後噴施不同濃度 BA 對文心蘭生長與開花的
影響……………………………………………….…..........14
試驗三 抽梗後噴施不同濃度硝酸鈣 Ca(NO3)2 對文心蘭
生長與開花的影響…………………………………………15
三、調查項目
(一)植株性狀調查………………………..……………………........15
(二)切花品質調查…………………………..…………………........15
(三)當代假球莖萌發營養芽調查……………………………..........16
四、分析方法………………………..……………………………………16
結果
ㄧ、抽梗後處理 kinetin 對文心蘭 ‘Honey Angel’ 生長、花序
發育及品質之影響………….…………………………………........17
二、抽梗後處理 BA 對文心蘭 ‘Honey Angel’ 生長、花序發育
及品質之影響………………………………………………………..28
三、抽梗後處理硝酸鈣對文心蘭 ‘Honey Angel’ 生長、花序發
育及品質之影響……………………..……………………………...40
討論
ㄧ、抽梗後處理 Kinetin 對文心蘭 ‘Honey Angel’ 生長、花序
發育及品質之影響……………………………..…………………...51
二、抽梗後處理 BA 對文心蘭 ‘Honey Angel’ 生長、花序發育
及品質之影響………………………..………………………………53
三、抽梗後處理硝酸鈣 Ca(NO3)2 對文心蘭 ‘Honey Angel’ 生
長、花序發育及品質之影響………………..………………………54
結論……………………..…………………………………………………...57
參考文獻…………………..………………………………………………...58

表目錄
表1. 葉面噴施kinetin一個月後對文心蘭 ‘Honey Angel’ 假球莖之
長、寬及厚度之影響……………………………...………..………..19
表2. 葉面噴施kinetin一個月後對文心蘭 ‘Honey Angel’ 假球莖
之葉長之影響.....................................................................................20
表3. 葉面噴施kinetin對文心蘭 ‘Honey Angel’ 開花及花序品質
之影響………………………..……………………………….……..21
表4. 葉面噴施kinetin對文心蘭 ‘Honey Angel’ 營養芽萌發之影響......22
表5. 葉面噴施kinetin對文心蘭 ‘Honey Angel’抽梗至第一朵花開
放及採收時間之影響………………………………………………...23
表6. 葉面噴施BA一個月後對文心蘭 ‘Honey Angel’ 假球莖之長、
寬及厚度之影響………………………………………….…….…….30
表7. 葉面噴施BA對文心蘭 ‘Honey Angel’ 假球莖之葉長之影響……31
表8. 葉面噴施BA對文心蘭‘Honey Angel’開花及花序品質之影
響.…………………………………………………………………......32
表9. 葉面噴施BA對文心蘭 ‘Honey Angel’營養芽萌發之影響.……….33
表10. 葉面噴施BA對文心蘭‘Honey Angel’抽梗至第一朵花開
放及採收時間之影響………………………………………………...34
表11. 葉面噴施Ca(NO3)2一個月後對文心蘭 ‘Honey Angel’假球
莖之長、寬及厚度之影響…………………………………..………42
表12. 葉面噴施Ca(NO3)2一個月後對文心蘭‘Honey Angel’假球
莖之葉長之影響…………………………………..………………...43
表13. 葉面噴施Ca(NO3)2對文心蘭 ‘Honey Angel’開花及花序品
質之影響……………………………………………………………44
表14. 葉面噴施Ca(NO3)2對文心蘭‘Honey Angel’營養芽萌發
之影響………………………………………………………………45
表15. 葉面噴施Ca(NO3)2 對文心蘭‘Honey Angel’抽梗至第一
朵花開放及採收時間之影響………………………………………46


圖目錄
圖1. 葉面噴施Kinetin對文心蘭‘Honey Angel’花梗長度變化
之影響……………………………………………………………….24
圖 2. 葉面噴施Kinetin處理之文心蘭‘Honey Angel’的花序外
觀…………………………………………………………………….25
圖 3. 葉面噴施Kinetin對文心蘭‘Honey Angel’切花吸水量變
化之影響……………………………………………..….……………26
圖 4. 葉面噴施Kinetin對文心蘭‘Honey Angel’的營養芽萌發
之影響……………………………………………………………….27
圖 5. 葉面噴施BA處理之文心蘭‘Honey Angel’的植株,營養
芽與花梗生長情形……………………………………………….......35
圖 6. 葉面噴施BA對文心蘭‘Honey Angel’花梗長度變化之影
響………………………………………………………………….......36
圖 7. 葉面噴施BA處理之文心蘭‘Honey Angel’的花序外觀……….......37
圖 8. 葉面噴施BA對文心蘭‘Honey Angel’切花吸水量變化之
影響……………………………………………………………..…...38
圖 9. 葉面噴施BA對文心蘭‘Honey Angel’的營養芽萌發之影
響………………………………………………………………..…...39
圖 10. 葉面噴施Ca(NO3)2 對文心蘭‘Honey Angel’花梗長度變
化之影響……………………………………………………….……47
圖 11. 葉面噴施Ca(NO3)2 處理之文心蘭‘Honey Angel’的花序
外觀……………….............................................................................48
圖 12. 葉面噴施Ca(NO3)2 對文心蘭‘Honey Angel’切花吸水量
變化之影響……………………………………………………..…...49
圖 13. 葉面噴施Ca(NO3)2 對文心蘭 ‘Honey Angel’的營養芽
萌發之影響………………………………………………………….

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