臺灣博碩士論文加值系統

本研究採用上流式厭氧污泥床(Up-flow Anaerobic Sludge Blanket, UASB)所馴養之厭氧污泥進行批次試驗，分別額外添加乙酸及丙酸，探討乙酸及丙酸之代謝路徑，其次應用代謝通量分析代謝路徑中反應物、中間產物、能量載體及離子等濃度之變化及反應速率，加以探討其代謝通量。結果發現，當額外添加乙酸時，其乙酸濃度變化由起始9769.04 mg/L，最終變為6930.67 mg/L，乙酸利用率為29%；額外添加丙酸時，其丙酸濃度變化由起始6580.98 mg/L，最終變為4704.79 mg/L，丙酸利用率為28.5%。乙酸之代謝路徑共有4條反應式，參與反應物質有16項；丙酸之代謝路徑共有12條反應式，其中參與反應物質有27項。乙酸之代謝通量，在第1天時，代謝通量為最大值，爾後趨於平緩，於第5天時，代謝通量有略顯升高，於第6天時，其代謝通量為最小值。丙酸代謝通量，當時間為第1天時，其代謝通量最大值，第2天後趨於平緩，於第5天時，其代謝通量為最小值。而在比較單位有機酸起始濃度及單位有機酸濃度下，額外添加乙酸與丙酸時，其通量皆在第1天為最大值，且開始大幅度下降，於第2天後下降趨勢減緩；能量載體方面，單位起始濃度及單位有機酸分解菌濃度下，除了第5天下外，丙酸之ATP、AMP及Pathway 3中H+，能量載體及離子之代謝通量為乙酸之1.30~2.61倍之間，顯示此三路徑丙酸之能量載體及離子代謝通量大於乙酸。另在單位起始濃度及單位有機酸分解菌濃度下，除了第6天下外，丙酸之NADH、Pathway 3中H+及NAD+，能量載體及離子之代謝通量為乙酸之0.48~0.84之間，顯示此三路徑丙酸之能量載體及離子代謝通量小於乙酸。

In this study, the metabolic flux analysis (MFA) was employed to explore the metabolic pathway and metabolic flux in the Up-flow Anaerobic Sludge Blanket (UASB) asnaerobic tank.
It was found that when the extra addition of acetic acid, acetic acid concentration changes from the starting 9769.04 mg/L, eventually becomes 6930.67 mg/L, acetic acid utilization was 29%; when adding additional acid, a propionic acid concentration changes by the start 6580.98 mg/L, eventually becomes 4704.79 mg/L, acid utilization rate of 28.5%. Metabolism acetic path total 4 reactive, substances involved in the reaction 16; propionic acid metabolic path of a total of 12 reaction formula, which reacted substances 27. Metabolic flux as acetic acid, in the first day, the metabolic flux is the maximum, and later leveled off in the 5th day, have slightly increased metabolic flux at the 6th day, their metabolic flux Min. Propionic acid metabolic flux, when the time for the first day, the maximum value of metabolic flux, the first two days after leveling off at 5th day, the metabolic flux to a minimum. When comparing unit while the starting concentration of organic acids and organic acid concentration units, additional acetic acid and propionic acid, which are flux is maximum at one day and began to fall dramatically, in the first two days of decline slowing ; energy carrier side, the initial concentration of units and unit bacteria, in addition to the fifth of the world outside, ATP propionic acid, AMP and Pathway 3 in H+, metabolic flux and ion energy carrier of between 1.3 to 2.61 times as acetic acid, displayed energy carrier and three ions metabolic flux path is greater than acetic acid. The other units in the unit and the initial concentration of the organic acid decomposing bacteria concentrations, in addition to Article 6 of the world outside, NADH propionic acid, Pathway 3 in H+ and NAD+, between 0.48 to 0.84 acetic metabolic flux of ions as an energy carrier and, the three show the path of the acid ion energy carrier and metabolic flux less than acetic acid.

摘要
Abstract
目錄
第一章 緒論
第一節 研究背景與動機
第二節 研究目的
第三節 名詞解釋
第二章 文獻回顧
第一節 厭氧微生物
第二節 厭氧醱酵酸化反應機制
第三節 厭氧微生物反應器
第四節 酸生成物之反應式
第五節 代謝通量分析原理
第六節 ADM1應用於厭氧槽中微生物功能族群之研究
第三章 研究方法
第一節 研究架構
第二節 研究流程
第三節 研究設備與材料
第四節 代謝通量分析
第五節 微生物功能族群量化
第四章 結果與討論
第一節 添加有機酸之厭氧反應槽操作情形
第二節 厭氧槽中有機酸代謝路徑之代謝通量分析
第三節 UASB厭氧槽中有機酸之微生物功能族群量化
第四節 單位有機酸起始濃度及單位有機酸分解菌濃度下之代謝通量
第五節 能量載體及離子之代謝通量
第五章 結論與建議
第一節 結論
第二節 建議
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