臺灣博碩士論文加值系統

本研究是來比較不透光的矽酸二鈣水泥（RDSC）和ProRoot白色三氧礦化物（WMTA）之間的細胞貼附、細胞毒性及抗菌活性。此外，本研究也藉由動物實驗分析RDSC和WMTA刺激骨再生的能力。實驗中利用AlamarBlue評估細胞貼附及生長的性質。在不同時間點的生長培養基中量測pH值變化進行測定。細胞毒性的評價根據ISO10993-5規範執行。使用糞腸球菌對於兩種材料的抗菌活性進行評估。最後將植入上述兩種材料的動物於一，三及六個月後予以犧牲，而完成組織學和生物化學的分析。在不同的培養時間，細胞培養於兩種材料上並沒有顯著的差異 （P>0.05）。在細胞毒性的分析中，在12和24小時的數據RDSC是顯著（P <0.05）優於WMTA 。通過抑菌圈和糞腸球菌抑菌率的結果可知RDSC和WMTA具有相似的抗菌活性。動物實驗結果也顯示：組織學測定用Masson染色顯示周圍的兔子股骨植入位點新形成骨的存在。組織化學的數據證明，在三至六個月RDSC組比WMTA組有表現出更多骨組織形成。在細胞貼附，細胞毒性和抗菌功能中，RDSC是可以媲美ProRoot WMTA。目前的研究結果表明，這種RDSC是可以用來作為根尖逆充填的材料。

To comparatively examine the cell attachment, cytotoxicity, and antibacterial activity of radiopaque dicalcium silicate cement (RDSC) and ProRoot white-coloured mineral trioxide aggregate (WMTA). In addition, this study compared the regenerative potential of RDSC and WMTAusing a rabbit femur model. AlamarBlue was used for real-time and repeated monitoring of MG63 cell attachment on freshly mixed and set cements. The pH changes in the growth medium at different time-points were also measured. Cytotoxicity evaluation was performed according to ISO 10993–5 specifications. The antibacterial activity of the cement specimens was evaluated using Enterococcus faecalis. The animals were sacrificed at one, three and six months to accomplish histological and biochemical analyses. There were no significant differences (P > 0.05) between the two cements for cell attach ment either in the fresh groups or in the set groups at all culture times. Neither freshly mixed group nor set groups had significant pH differences. In the case of cytotoxicity, RDSC was significantly (P < 0.05) superior to WMTA at 12 and 24 h of incubation. RDSC and WMTA possessed similar antimicrobial activity, substantiated by the formation of growth inhibition zones and bacteriostasis ratio in E. faecalis strains. The animal study results indicated that after one month of implantation, WMTA was associated with a greyish color alteration within its mass, while RDSC presented color stability even at six months. Histological assay with Masson’s Trichrome and Von Kossa stains showed the presence of newly formed bone surrounding the implanted sites in the rabbit femur. The histochemical data revealed that the RDSC group had significantly more bone regeneration than did the WMTA groups at three and six months. The cell attachment, cytotoxicity and antibacterial efficacy of RDSC were comparable to those reported for ProRoot WMTA. The results of the current study suggest that this RDSC could be used as a root-end filling material and root sealer.

Abstract i
Chinese Abstract ii
Contents iii
List of Figures vi

Chapter 1 Introduction 1
1.1 Bone cement 1
1.2 Si effect 1
1.3 Silicate-based materials 3
1.3.1 Bioglass 4
1.3.2 Mineral trioxide aggregate 4
1.3.3 Calcium silicate cement 5

Chapter 2 Materials and methods 8
2.1. Specimen preparation 8
2.2. Cell attachment 9
2.3. Cytotoxicity 10
2.4. Antibacterial activity 11
2.5. Animals experiment 12
2.5.1. Surgery 12
2.5.2. Color Stability 13
2.5.3. Histological Assay 13
2.5.4. Western blot 14
2.6. Statistical analysis 15

Chapter 3 Results 16
3.1. Cell attachment 16
3.2. Cytotoxicity 16
3.3. Bacteriostasis ratio 17
3.4. Zone of inhibition 17
3.5. Animals experiment 17
3.5.1. Inflammatory Response 17
3.5.2. Degradation 18
3.5.3. Color Stability 18
3.5.4. Histological Observation 18
3.5.5. Bone-Related Protein Expression 19

Chapter 4 Discussion 21

Chapter 5 Conclusions 30

Reference 43

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