臺灣博碩士論文加值系統

紫外線是一種輻射線，會造成皮膚的老化，並且導致皮膚病理的改變。紫外線A被認為是老化的輻射線，也是氧化壓力的主要的來源之一，會導致人類皮膚的光老化。在過去，已經有許多研究證明皮膚細胞經過紫外線的照射後會產生活性氧族，而分裂原活化蛋白激酶路徑也會被活化，並且導致人類皮膚細胞內c-Jun N端激酶（JNK）、p38激酶（P38）、細胞外訊息調節激酶（ERK）等蛋白的磷酸化。Cycloheterophyllin屬類黃酮化合物，從麵包樹根皮分離出來的成分，在過去的研究指出，cycloheterophyllin具有抗氧化的作用，可以有效地清除自由基；然而cycloheterophyllin雖然已在多項研究中被報導，但是對於人類皮膚細胞光老化的保護作用，其分子機轉還沒有研究報導。因此，我們利用人類真皮纖維母細胞去探討cycloheterophyllin對紫外線誘發傷害的保護作用。我們發現，cycloheterophyllin可明顯減少因照射UVA後造成之細胞死亡，並且減少細胞在UVA照射後的MAPK磷酸化作用；此外，cycloheterophyllin還可以降低細胞內過氧化氫之生成。在活體研究中，我們發現，以cycloheterophyllin做局部的應用，可以有效的減少紫外線A照射後皮膚經皮水分散失、皮膚發紅還有水腫等現象，並且使得血流的速度下降。這些結果證明，cycloheterophyllin的光保護作用是可以有效抑制UVA誘導產生的氧化壓力和傷害，且在預防皮膚光老化方面是有益的。

Ultraviolet (UV) ray is one of an irradiation, which cause of skin aging and results in pathological changes in the skin. UVA is considered as the aging ray, which is also a significant source of oxidative stress, which causes photoaging in human skin. In the past, many studies have documented that the skin cells produce reactive oxygen species after UV exposure. The mitogen-activated protein kinase (MAPK) pathway would be activated, resulting in phosphorylation of JNK, ERK, and p38 kinase in human skin cells. Cycloheterophyllin, a prenylflavone, is isolated from the root bark of Artocarpus Heterophyllus. The past studies demonstrated that cycloheterophyllin has an antioxidant effect and can effectively scavenge free radicals. Although cycloheterophyllin had been studied, the molecular mechanisms by which on their protection of human skin cells during the photoaging have not been investigated. Therefore, we used human dermal fibroblasts to investigate protective effect of cycloheterophyllin on UV-induced damage. We found that cycloheterophyllin significantly increased cell viability and attenuated the phosphorylation of MAPK after UVA exposured. Furthermore, cycloheterophyllin could reduce H2O2 generation. In the in vivo studies, we found that the topical application of cycloheterophyllin before UVA irradiation significantly decreased the value of trans-epidermal water loss（TEWL）, erythema, corneometer and the speed of blood flow. These results indicate that photoprotective effects of cycloheterophyllin inhibited UVA-induced oxidative stress and damage, and may be beneficial in the prevention of skin photoaging.

Abstract 1
摘要 2
第一章　緒言 3
第二章　文獻回顧 7
第三章　研究動機與目的 28
第四章　實驗材料與器材 30
第五章　研究方法 33
第六章 研究結果 46
第七章 討論 58
第八章 結論 63
參考文獻 82
文獻回顧圖示 V
實驗結果圖示 VI

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