臺灣博碩士論文加值系統

中草藥如秦皮、黃連、苦參、黃柏等具有止瀉的功效，大黃、巴豆、牽牛子、火麻仁等具有瀉下的功效。由於腹瀉與止瀉的生理機制與腸道上皮間之離子通透及水分進出有關，本研究即探討上述具止瀉及瀉下功能的中藥酒精及水抽出物對於腸道上皮細胞離子運送的影響，實驗方法主要利用Ussing Chamber連結voltage/current clamp的技術測量大白鼠腸道上皮組織因離子移動產生的電流變化。第一部份實驗探討這些中草藥酒精及水抽出物對電流的影響，結果顯示，在於serosal端加入5μM forskolin引發氯離子分泌，使電流上升之後，再於serosal端加入中藥材酒精抽出物，秦皮、苦參、黃連、黃柏、鴉膽子等止瀉中草藥酒精抽出物能夠降低forskolin引發之上升電流，而具有瀉下功效之大黃、巴豆、牽牛子、火麻仁等中藥酒精抽出物則使forskolin引發之上升電流再增加。這些藥材水抽出物對電流的影響則較酒精抽出物為低。第二部分實驗係利用與鈉離子主動運輸有關的Na+-K+ ATPase抑制劑 Ouabain，及與氯離子主動運輸有關的Na+-K+-2Cl- cotransporter 抑制劑 Bumetanide，探究這些藥材酒精抽出物與鈉離子及氯離子移動的關係，結果發現大黃、巴豆、秦皮、火麻仁等中草藥酒精抽出物可能是藉由影響氯離子的運送而使離子電流產生變化，而苦參可能是藉由影響鈉離子的運送而使離子電流產生變化。本研究結果初步證實所探討之中草藥可能是藉由影響腸道上皮細胞離子運送的改變因而具有止瀉或瀉下功能。

According to the chinese medical literature, C. teeta Walich, Fraxini cortex, Goldenseal, Sophora flavescens Aiton, and Phellodendron amurense Rurp are known for the anti-diarrheal characteristics, whereas Rheum palmatum L., Croton tiglium L., Cannabis sativa L. and Pharbitis hederacea Choisy are known for the ability to promote diarrhea. This study was aimed to discern effects of these herbs’ extracts on ion transport of the rat intestinal epithelia. The epithelia were mounted in the Ussing chamber and were attached to voltage/current clamp for measuring changes of the short circuit current. After serosal addition of 5μM forskolin to raise the current, ethanol-extracts and water-extracts of the herbs with anti-diarrheal characteristics did reduce the current. On the contrary, ethanol-extracts and water-extracts of the herbs with diarrhea-promoting characteristics augmented the current further. Moreover, ethanol-extracts of these herbs showed more effect on changing the current. In following experiments, ouabain and bumetanide, which inhibit Na+-K+-ATPase and Na+-K+-2Cl- cotransporter respectively, were added prior to ethanol-extracts of these herbs for revealing the herb’s effect on Na+ and Cl- movement. The results suggest Fraxini cortex, Rheum palmatum L. and Croton tiglium L.，Cannabis sativa L. would affect Cl- transport, whereas Sophora flavescens Aiton would affect Na+ transport. This study provide evidences to confirm that changes of Cl- or Na+ transport may be involved in cellular activities of the anti-diarrheal and diarrhea-promoting herb medicines on the rat intestinal epithelia.

致謝-----------------------------------------------------1
中文摘要-------------------------------------------------2
目錄-----------------------------------------------------3
表目錄---------------------------------------------------4
圖目錄---------------------------------------------------5
壹、前言-------------------------------------------------7
貳、材料與方法-------------------------------------------17
參、結果-------------------------------------------------22
肆、討論-------------------------------------------------27
伍、參考文獻---------------------------------------------33
英文摘要-------------------------------------------------58
作者簡歷-------------------------------------------------60
著作權聲明-----------------------------------------------61

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