臺灣博碩士論文加值系統

本研究設計一套量測生物體機械性質的方法，此方法主要由微玻
管搭配AFM 探針做為主要設備，逐漸拉伸生物體直到破壞，再由AFM
探針的彎曲可以得到其生物體的機械性質。
此實驗主要生物體是肌肉微管細胞(muscle myotubes)，此細胞
是由肌肉原細胞(muscle myoblasts)分化而得，肌肉微管細胞為肌肉
組成最基本單位，此實驗可幫助研究或計算肌肉組織的強度作為參考
文獻。

In this study, we developed a device to measure mechanical
property of biomolecules. It mainly utilized the micropipette
and AFM probe for the measurement, and it can stretch the cell
from its initial length to the ultimate break point. We
calculated the stretching force from the bending deflection of
the AFM probe.
Myotubes are the objects in this study. Myotubes are the
basic unit cell in muscular structure and are derived of
myoblast differentiation. The measurement and understanding of
myotube strength is the foundation from which to investigate
the mechanical properties of muscle.

摘要 ..................................................... I
ABSTRACT ................................................ II
誌謝 ................................................... III
目錄 .................................................... IV
圖目錄 .................................................. VI
表目錄 .................................................. IX
第一章 緒論 .............................................. 1
第二章 生物體量測 ........................................ 3
2.1、 細胞分化與培養 .................................. 3
2.2、 文獻回顧 ........................................ 4
2.2.1. 生物分子量測方法 .............................. 4
2.2.2. 量測方法比較 .................................. 8
第三章 實驗設備 ......................................... 10
3.1、 實驗設備 ....................................... 10
3.2、 壓力校正 ....................................... 20
3.2.1. 量測尺度轉換 ................................. 20
3.2.2. 固定乳膠微球至AFM 懸臂樑 ...................... 23
3.2.3. 吸力校正 ..................................... 25
第四章 實驗方法與實驗結果 ............................... 32
4.1、 實驗原理 ....................................... 32
4.2、 實驗準備 ....................................... 34
4.2.1. 細胞培養 ..................................... 34
4.2.2. 實驗步驟 ..................................... 38
4.3、 實驗結果 ....................................... 42
4.3.1. 細胞黏附力實驗過程及分析 ...................... 42
4.3.2. 微管細胞的抓取 ............................... 49
4.3.3. 微管細胞拉伸實驗 ............................. 51
4.3.4. 微管細胞的量測 ............................... 54
4.3.5. 微管細胞拉伸實驗分析.......................... 59
4.3.6. 微管細胞老化 ................................. 60
第五章 實驗討論與結論 ................................... 62
5.1、 實驗討論 ....................................... 62
5.2、 實驗結論 ....................................... 63
參考文獻 ................................................ 64

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