臺灣博碩士論文加值系統

近年來，肥胖已成為全球重視的一項公共衛生問題。肥胖是罹患心血管疾病、糖尿病與高血壓等疾病的危險因子。從能量平衡的角度來看，肥胖被認為是多餘的能量以脂肪的形式累積於脂肪組織的情形，因此造成體重逐漸增加。許多研究指出天然植物中所含的的化學成分具有抑制脂解酵素、α-澱粉酶與α-葡萄糖苷酶的功能。而利用中草藥的天然成分來改善人體健康已受到相當的重視。本研究從坊間中草藥店隨機選取57種中草藥作為實驗材料，以水萃取之方式，經由冷凍乾燥濃縮後，利用抑制酵素分析方法從中選取具有抑制脂解酵素、α-澱粉酶與α-葡萄糖苷酶能力的中草藥。經抑制酵素實驗結果發現，57種中草藥大部分對三種酵素都有抑制的效果，有少部分對於酵素有促進活性的效果，而E14中草藥對此三種酵素有最佳抑制效果。E14以濃度0.15625 X進行抑制實驗有最佳效果，對脂解酵素、α-澱粉酶與-葡萄糖苷酶的抑制效果分別為80、106與94%。為瞭解萃取物與酵素間之作用關係，使用E14萃取物進行酵素競爭試驗，發現E14與脂解酵素及α-澱粉酶之間的關係是屬於非競爭型抑制，與-葡萄糖苷酶則為不競爭型。總多酚含量實驗發現，E14在57種中草藥之中，並不是總多酚含量最多之中草藥。為探討何種成分具抑制作用，進而以HPLC去分析E14中草藥萃取物中的成分。HPLC分析實驗選用C18管柱去分析E14中草藥萃取物，並以波長210 nm 為偵測波長，每分鐘收集分層液，再以不同分層液進行酵素抑制實驗。結果發現，在53-54 min 這個分層液對脂解酵素、α-澱粉酶與α-葡萄糖苷酶之抑制率分別為59.36 98.81與95.02%。由以上結果可得知E14這個中草藥之水萃取物，可以抑制脂解酵素、α-澱粉酶與α-葡萄糖苷酶，其抑制能力與E14中草藥中所含的特定成份有關係。

In recent years, obesity has become a sanitary problem that the global people put emphasis on. Obesity is a bad factor associated with cardiovascular diseases, diabetes, high blood pressure, and so on. Because of imbalance of intake and expenditure, obesity is thought of the condition that the excess energy is converted into fat, which is then accumulated in adipose tissues. Therefore, it causes gradual weight gain. Many studies showed that chemicals in the natural products may function in inhibiting lipase, α-amylase and α-glucosidase to decrease lipid accumulation. Recently, it is greatly emphasized on using the natural chemicals in the Chinese herbs to help improve people health. In this study, we collected fifty seven Chinese herbs from stores as materials. We used freeze-dried concentrated water extracts of herbs and the enzyme inhibition analysis to search for Chinese herbs that inhibit lipase, α-amylase and α-glucosidase. The results of enzyme inhibition analysis showed that most Chinese herbs represent inhibiting effects on these three enzymes, whereas a few herbs have adverse effects, increasing the activity of some enzymes. One of the fifty seven Chinese herbs, E14, exerts most inhibition activity on these enzymes. When using dilution factor of 0.15625X, the inhibition rates of lipase, α-amylase and α-glucosidase are 80, 106 and 94%, respectively. To address whether the inhibition is competitive, noncompetitive or uncompetitive, we performed competitio tests and found that the inhibition effects of E14 on lipase and α-amylase are uncompetitive and, however, effect on α-glucosidase is noncompetitive. The measurements of total polyphenol content of these herbs showed that E14 is not the richest. Therefore, we used the HPLC to analyze the ingredients of Chinese herbal extracts. We used C18 column and ultraviolet–visible spectroscopy on wavelength of 210 nm to detect the ingredients. Then we collected the analyzed fraction per minute for the enzyme inhibition experiments. The results showed that fraction during 53-54 min has 59.36, 98.81 and 95.02% of inhibition rates on lipase, α-amylase and α-glucosidase, respectively. The conclusion is that the water extract of Chinese herb E14 can inhibit lipase, α-amylase and α-glucosidase and the functions are associated with some of its ingredients.

中文摘要………………………………………………………………………ii
英文摘要 ……………………………………………………………………iii
壹、導論…………………………………………………………………………1
貳、研究構想………………………………………………………………16
參、材料與方法…………………………………………………………18
肆、結果………………………………………………………………………29
伍、討論與結論……………………………………………………………35
陸、參考文獻………………………………………………………………39
柒、表……………………………………………………………………………39
捌、圖……………………………………………………………………………51
玖、附表…………………………………………………………………………50
拾、附圖…………………………………………………………………………51

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