臺灣博碩士論文加值系統

紅龍果（Hylocereus undatus (Haw.) Britton et Rose）又名火龍果、仙蜜果、量天尺，為仙人掌科（Cactaceae）。火龍果品種依果肉顏色可區分成三種，分別是紅皮白肉的火龍果(Hylocereus undatus）、紅皮紅肉的紅龍果(Hylocereus polyrhizus）以及黃皮白肉的黃仙蜜果(Hylocereus megalanthus）。科學研究指出紅肉火龍果果皮富含以甜菜紅素(betacyanin)為主之甜菜色素(betalains)，具有抗氧化及消除自由基等生化活性，本研究計畫將探討紅肉火龍果果皮萃取物之免疫調節功能。本研究計畫完成以水、50%或95%酒精溶液萃取之紅肉紅龍果果皮萃取物(Hylocereus polyrhizus fruit peel extract; EHPFP)之製備，並利用小鼠初代脾臟及腹腔細胞，評估各萃取物對小鼠免疫細胞之存活率及免疫調節作用。實驗結果顯示所使用之樣品濃度(31.5~2000 μg/ml)未妨礙小鼠脾臟初代細胞之生長，50%及95%酒精萃取物的部分濃度樣品(50%EHPFP: 250和500 μg/ml；95% EHPFP: 31.25、500~2000 μg/ml)顯著地(p <0.05)提高細胞之存活率。高濃度(2000 μg/ml)之紅肉紅龍果果皮水萃物(WEHPFP)顯著地(p < 0.05)提高在正常模式下小鼠腹腔細胞分泌之促發炎/抗發炎細胞激素(IL-6/IL-10)之比值，而95%酒精萃取物則是降低IL-6/IL-10的比值。樣品濃度低於500 μg/ml之水及50%酒精萃取物，顯著地(p＜0.05)提高Con A-stimulation模式下脾臟細胞分泌之Th1/Th2-type細胞激素比值(IFN-γ/IL-5與IFN-γ/(IL-4+IL-5))，促使Th1/Th2-type細胞免疫平衡傾向Th1端，但高濃度(2000 μg/ml)之50%及95%酒精萃取物則顯著地(p＜0.05)降低Th1/Th2-type細胞激素比值，使Th1/Th2-type細胞免疫平衡往Th2端移動。水萃取樣品(250~2000 μg/ml)顯著地(p＜0.05)增加正常模式下IL-6/IL-10比值，而高濃度(2000 μg/ml)之水及50%酒精萃取物則顯著地(p < 0.05)降低LPS-stimulation模式下IL-6/IL-10比值。綜合以上結果推論三種紅肉紅龍果皮萃取物對小鼠脾臟初代細胞不具有細胞毒性，紅肉紅龍果果皮水萃物具有促發炎作用，而高濃度(2000 μg/ml)之萃取物則有抗發炎的潛力，以及紅肉紅龍果果皮萃取物對小鼠脾臟輔助型T細胞得免疫調節作用與其及性和濃度有關。

Pitaya (（Hylocereus undatus (Haw.) Britton et Rose）) or dragon fruit is native to Central and South America and has been grown in Taiwan for at least 20 years. A member of the Cactaceae, it has trailing cladode stems modified to act as leaves and bears spectacular ovoid fruit year-round which has a bright red color when mature, and contains white, crimson, or pale yellow flesh, depending on the cultivar, interspersed with small black seeds. Hylocereus polyrhizus, a red pitaya with red flesh, is widely grown in Taiwan. Pitaya fruit has been reported as a source of beta-carotene, lycopene and vitamin E. The recent studies showed that betacyanin, a major component of betalains, which rich in Hylocereus polyrhizus fruit peels exhibited higher antioxidant activities. The aims of the present study were to investigate the anti-inflammatory compounds and mechanisms of Hylocereus polyrhizus fruit peels (HPFP) which extracted by water, 50% or 95% ethanol solution on primary peritoneal cells from BABL/c mice.

中文摘要 I
英文摘要 II
目 錄 III
表目錄 IV
圖目錄 V
第一章 緒論 1
第二章、文獻回顧 4
第三章、研究動機與目的 8
第四章、材料方法 11
1. 試藥 12
2. 紅肉紅龍果果皮萃取物樣品溶液之製備 12
3. 樣品溶液之製備 13
4. 初代免疫細胞之來源 13
3. 小鼠脾臟細胞存活率之評估 14
4. 小鼠腹腔細胞正常及誘發發炎反應模式 15
5. 小鼠脾臟細胞正常及誘發發炎反應模式 16
6. 腹腔細胞及脾臟細胞分泌細胞激素之測定 16
7. 統計分析 17
第五章、結果 18
第六章、討論 35
第七章、總結論 38
第八章、參考文獻 39

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