臺灣博碩士論文加值系統

本研究主要目的同時且快速分離七種美白劑，以離子對層析法(IPC)層析方式進行測定，探討維生素C及其三種衍生物、熊果素、對苯二酚、麴酸在不同pH值緩衝溶液、有機溶媒修飾劑的體積、離子對試劑等影響分離參數，以找出最佳分離條件，選擇最適合的層析方法進行實驗。以C18管柱做為靜相，線性方程式相關系數(R2)均在0.999以上。樣品回收率97.61-104.78％。並評估七種美白劑溶解在不同pH值溶劑下的安定性。

The purpose of this study is to separate simultaneously seven water soluble whiten agents by ion pair chromatographic method. These components include ascorbic acid and its three derivative; arbutin, hydroquinone and kojic acid. The parameters of IPC for separation, such as pH value of buffer, organic modifier composition and ion pair reagent concentration, were studied in this research. The results showed these seven compounds could be well separated under optimum condition by using C18 column as stationary phase. The regression coefficient of all compounds calibration curve are larger than 0.999.
The stability of these components in water of different pH values were also investigated. This method is applied for determination of these compounds in lotions by simple dilution. Recoveries of the six analytes were between 97.61-104.78%.

摘要 I
Abstract II
致謝 III
目錄 IV
表目錄 VII
圖目錄 VII
第一章 緒論 1
1-1前言 1
1-2研究動機與目的 2
1-3研究架構 3
第二章 文獻回顧 5
2-1皮膚構造 5
2-1.1黑色素細胞 6
2-1.2黑色素生成機轉 6
2-2美白劑在化妝品中使用限量 10
2-3美白劑介紹 11
2-4美白劑相關之研究 16
第三章 實驗材料與方法 25
3-1實驗材料 25
3-2儀器設備 27
3-3研究方法 28
3-3.1波長選擇 28
3-3.2離子對試劑濃度的選擇 28
3-3.3探討不同有機修飾劑比例 29
3-3.4探討不同緩衝溶液pH值 29
3-4標準品溶液及檢量線配製 30
3-5自製、市售樣品前處理及添加方法 30
3-6安定性評估 33
3-7精密度與準確度 35
3-8方法偵測極限(LOD)及定量極限(LOQ) 35
第四章 結果與討論 36
4-1七種美白劑紫外線吸收波長探討 36
4-2七種美白劑穩定性評估 38
4-3不同濃度TBAH離子對試劑影響各美白劑層析結果之探討 40
4-4不同比例有機修溶劑影響各美白劑層析結果之探討 43
4-5不同pH值緩衝溶液影響七種美白劑層析結果之探討 47
4-6檢量線建立 50
4-7方法偵測極限(LOD) 及定量極限(LOQ) 52
4-8精密度與準確度 53
4-8.1自製樣品含量測試及添加回收率 53
4-8.2自製樣品安定性評估 53
4-8.3異日間分析(Inter-day)、同日間分析 (Intra-day) 55
4-8.4市售樣品測定 55
第五章 結論 61
參考文獻 62

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