臺灣博碩士論文加值系統

鼻淋巴瘤（Nasal and nasal type T/NK cell lymphoma）是淋巴瘤中最惡性的一種。此種淋巴瘤發生的位置大部分在鼻腔，或是面部中線。而且，它和EBV（Epstein Barr Virus）有很高的相關性。在亞洲人及印地安人中，此種淋巴瘤發生的機率遠大於高加索人，且約有四分之三是發生於男性。除此之外，它的預後很差，復發率極高，這些都是引發我們想去研究了解它的原因。而在此，我們想了解它的染色體變異以進一步研究鼻淋巴瘤的形成，我們所運用的技術是基因組比對雜交（CGH : Comparative Genomic Hybridization）。
CGH技術原理是將等量癌細胞及正常細胞之DNA分別標定上不同螢光染劑後，同時與玻片上正常中期染色體雜交。經由分析兩種螢光在中期染色體上的比例可得知癌細胞DNA在染色體上有無擴增（Gain）或缺失（Loss）的片段。而這些擴增或缺失片段就可能是致癌基因（oncogene）或抑癌基因（tumor suppressor gene）所在的位置。我們用CGH分析了17例由成大醫院提供的鼻淋巴瘤DNA樣本，以及一株細胞株HANK1（日本 Dr. Kagami提供）。結果顯示鼻淋巴瘤染色體變異最主要是在X染色體上的擴增（29.4 %）。
在此之前鼻淋巴瘤染色體變異的研究較少，主要都是以傳統細胞遺傳學方法核型分析來研究。他們的研究結果顯示在鼻淋巴瘤染色體變異中 染色體6q21-25片段的缺失是較共通的現象，而在我們CGH研究結果中並無發現此片段的缺失。至於X染色體的變異是之前研究和現在CGH的發現共同點，是否在X染色體上的變異跟男女發生鼻淋巴瘤比率不同有關，抑或是有其他的抑癌基因或致癌基因在X染色體上，值得我們將來更深入的研究探討。

Nasal lymphoma (nasal and nasal type T/NK cell lymphoma) is the most aggressive kind of lymphoma. It was most often occurred in nose or facial midline. And it is highly associated with Epstein Barr Virus. The incidence of nasal lymphoma in Asian and Native American are much higher than that of Caucasian. There are about three forth cases occurred in male. Besides, the prognosis of nasal lymphoma was very poor, and there was highly rate of relapse. Because of these reasons, we are interest in analyzing how it happens. Here, we want to realize the cytogenetics of nasal lymphoma by using the CGH (Comparative Genomic Hybridization) technique to achieve it.
The principle of CGH technique is that labeling the equal amount DNA of cancer cells and that of normal cells with different fluorescence dye, and then hybridizing to normal metaphase chromosomes on the slide at the same time. By analyzing the ratio of fluorescence on metaphase chromosomes, we can realize that are there any gain or loss segments of DNA of cancer cells in chromosomes. And there were most likely oncogenes or tumor suppressor genes in these gain or loss segments. We analyzed 17 cases DNA of nasal lymphoma from NCKU, and one cell line -- HANK1 (offered by Dr. Kagami in Japan) by using CGH. The result indicated that the mainly chromosomal aberration is gain in X chromosome (29.4%).
There were few researches done about chromosomal aberrations of nasal lymphoma before, and they were using conventional cytogenetic technique (karyotyping). The results have a common finding that the loss of chromosome 6q21-25 segment in the chromosomal aberrations of nasal lymphoma, but it did not appear in our CGH results. The X chromosomal aberrations are the common findings both in researches using earlier cytogenetic technique and our CGH experiments. Is there any oncogene or tumor suppressor gene on X chromosome? This is a good question for further research.

中文摘要 -------------------------- 1
英文摘要 -------------------------- 3
緒 論 -------------------------- 5
材料與方法------------------------ 10
結 果 ------------------------- 21
討 論 ------------------------- 24
參考資料 ------------------------- 30
附 表 ------------------------- 35
附 圖 ------------------------- 40

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