臺灣博碩士論文加值系統

縐葉菸草 ( Nicotiana plumbaginifolia ) 有十對末端中節或近端中節的染色體，
利用傳統細胞學的技術無法輕易地將每一對染色體加以區別。利用螢光原位雜交
( fluorescence in situ hybridization, FISH ) 的技術，藉著一個稱為NP3R的
重複性DNA序列在染色體上的分佈，建立N. plumbaginifolia的核型。NP3R是一個
165 bp頭尾相接的重複性序列 ( tandemly repetitive sequence ) ，在十對染色
體上的分佈由於有位置和數量的差異，故能清楚地鑑別N. plumbaginifolia的每一
對染色體。此外，利用原位雜交重複標定和多顏色螢光原位雜交 ( multi-color
FISH ) 的方法，將5S rRNA基因定位於第二對染色體長臂的近端，26S rRNA基因定
位在第九對及第十對染色體的末端。

Nicotiana plumbaginifolia has 10 pairs of acrocentric or telocentricchromosome
s, which could not be distinguished from each other by conventional cytologica
l techniques. The somatic karyotype of this species was constructed based on f
luorescence in situ hybridization (FISH) to a 165 bp tandemly repeated sequenc
e, NP3R. Variation in the spatial and quantitative distribution of this repeat
allowed the identification of each of the 10 pairs of N. plumbaginifolia chr
omosomes. Two-step FISH and multi-color FISH, showed that 5S rDNA was clustere
d in a proximal region in the long arm of chromosome 2, and that 26S rDNA was
clustered in the terminal regions of chromosomes 9 and 10.