臺灣博碩士論文加值系統

傳統中醫經絡理論認為十二經脈內屬於臟腑，外絡肢節，脾經聯絡於脾臟，而肝經則與肝臟相聯絡，即所謂經脈的特異性。因此，本研究的目的是探討十二經脈的特異性。我們分別於Spragrue-Dawley大鼠的脾經合穴陰陵泉（SP9）和肝經合穴曲泉（LR8）施予 2 Hz電針刺激，使用雷射都卜勒血流測定儀同時記錄肝臟和脾臟血流。實驗分為假電針組，陰陵泉組和曲泉組，血流測定分為電針前期紀錄（baseline紀錄）、電針期紀錄和電針後期紀錄，每期記錄10分鐘，並於每期記錄完成時立即分別從右側股動脈採血測量一氧化氮（nitric oxide, NO）濃度。結果顯示假電針不能增加肝臟或脾臟血液量，而2 Hz電針兩側的陰陵泉能增加脾臟，但不能增加肝臟的血流量；相反的，2 Hz電針兩側曲泉能增加肝臟，但不能增加脾臟血流量。各組三期之間的NO濃度改變，未達統計學上的顯著。
本研究的結果可以說明不同經絡之合穴對其經絡之聯屬內臟具有特異性，同時也印證傳統中醫經絡理論認為經氣從合穴進入本經之經脈，以及經脈是內連臟腑。

The meridian theory of Traditional Chinese medicine considers that twelve meridians belongs to viscera and bowels internally, and connects to limbs and joints externally. Spleen meridian connects to spleen, and liver meridian connects to liver, i.e. so call meridian specificity. Therefore, the purpose of the present study was to investigate specificity of twelve meridians. We applied 2 Hz electroacupuncture（EA）to Yinlingquan（SP9）acupoint that is a sea acupoint of spleen, and Ququan（LR8）acupoint that a sea acupoint of liver meridian, respectively. The blood flow of spleen and liver was recorded simultaneously by a laser Doppler blood flow monitor. The experiment was divided into sham, Yinlingquan, and Ququan groups, and blood flow recordings were divided into before EA period（baseline period）, EA period and post-EA period, each period was 10 min recordings. Nitric oxide levels（NO）were measured at finishing before EA period, EA period and post-EA period recordings from right femoral arterial blood. Results indicated that sham group could not increase the blood flow of liver and spleen; 2 Hz EA applied to bilateral Yinlingquan acupoints could increase the blood flow of spleen, but could not increase the blood flow of liver; In contrast, 2 Hz EA applied to bilateral Ququan acupoints could increase the blood flow of liver, but could not increase the blood flow of spleen; NO levels were not significantly difference among three periods in each group.
Based on these results, the sea point of different has specificity to its connecting viscera, these results also proof the qi of meridian is from sea point into meridian and the meridian connects to its viscera.

第一章 前言 1
第二章 文獻探討 3
2.1. 中醫對血及微循環的認識 3
2.1.1 血的功能 3
2.1.2 中醫的經脈系統 4
2.1.3 微循環（microcirculation） 5
2.2. 針灸對氣血的影響 5
2.3. 針灸對自主神經系統及內臟功能的影響 6
2.3.1 針灸可以激發訊息的傳遞 6
2.3.2 針灸對胃蠕動（gastric motility）的影響 7
2.3.3 針灸對膀胱排尿的影響 7
2.3.4 針灸對腎上腺髓質功能的影響 8
2.3.5 針灸對心血管系統的影響 8
2.4. 針灸對血流之影響 9
2.4.1 針灸對腦血流的影響 9
2.4.2 針灸對子宮血流的影響 10
2.4.3 針灸對腎血流的影響 10
2.4.4 針灸對骨骼肌血流的影響 11
2.5. 陰陵泉與曲泉之部位、功效與主治 11
2.5.1 陰陵泉（國際代碼 SP9）之位置、解剖與功效主治 12
2.5.2 曲泉（國際代碼 LR8）之位置、解剖與功效主治 13
2.6. 雷射都卜勒血流測量儀（Laser Doppler Flowmetry） 15
第三章 材料與方法 17
3.1. 動物（animals） 17
3.2. 實驗流程（experimental procedure） 17
3.3. 血流量的測定（measurement of blood flow） 17
3.4. 分組（grouping） 18
3.4.1 假電針組（sham group, SG） 18
3.4.2 陰陵泉組（Yinlingquan group, Y G） 18
3.4.3曲泉組（Qūquán group, QG） 19
3.5. 動脈血氣體分析及NO的測定 19
3.5.1. 動脈血氣體分析 19
3.5.2. NO的測定 19
3.6. 統計分析（statistical Analysis） 21
第四章 結果 22
4.1. 動脈血氣體分析 22
4.2. 電針對NO濃度的影響 23
4.3. 假電針對血流量的影響 23
4.4. 陰陵泉電針對血流量的影響 24
4.5. 曲泉電針對血流量的影響 25
4.6.電針不同穴位血流改變之比率 26
第五章 討論 28
第六章 結論 30
參考文獻 31
英文摘要 37

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