臺灣博碩士論文加值系統

以長期保存由人參根部所誘導之胚性癒合組織建立懸浮培養系統。
在1/2 MS基本鹽類的培養基中，加入5%的椰子水及casein hydrolysate，
培養一個月後，可得到芽狀體的分化，培養2個月可得均勻一致之含芽癒
合組織，將培養液中癒合組織密度降低，可使癒合組織體積增加，芽狀物
增加。將懸浮培養 2個月所得之芽狀物移至含GA及BA 各0.5或1 ppm 的B5
固態培養基，可得簇生狀的植株；將之移至含2,4-D 1ppm 之MS 固態培養
基，1 個月後有胚狀體的發生。
懸浮培養時，含 3 ppm以上高濃度的 2,4-D 的 MS 培養基，對於
人參胚性癒合組織的生長有抑制的作用。但在含 1 ppm kinetin 或 5%
椰子水的1/2 MS培養基中，高濃度的2,4-D抑制效果明顯減低。0.1至0.5
ppm的PPT有誘導胚性癒合組織增加的效果，但 1ppm 以上的PPT會使癒合
組織停止生長，褐化死亡。含GA及BA 各1ppm 的B5修正培養基可使高密度
培養的胚性癒合組織在短期內發芽。低溫下(4℃)培養兩天的細胞團，在
常溫下其活性略低，較易褐化。平板培養中胚狀體或芽、根狀物的發生時
間與細胞團本身的大小及胚狀體的成熟度密切相關，亦與固體培養基的配
方有關。

Embryogenic cell suspension culture was established from long
-time cultured root drieved embryogenic callus. Embryogenic
callus cultured in modified MS liquid medium with 5% coconut milk
and 0.5 g/l casein hydrolysate produced shoots.The shooting
callus transfered to B5 solid medium which is contained 1 ppm GA
and 1 ppm BA produced green plants with normal shoots and roots.
Transfer the callus to MS solid medium contained 1 ppm 2,4-D will
induce somatic embryo formation.MS liquid medium with more than
3ppm 2,4-D depressed the embryogenic cluster formation. Coconut
milk or 1 ppm kinetin did not depress the growth of the embyogenic
clusters.
Low concentration of PPT (0.1 to 0.5 ppm ) in MS liquid medium
had promotive effect in embryogenic clusters formation. PPT at
high concetration (more than 1 ppm) retarded growth of callus. B5
liquid medium contain 1 ppm GA and 1 ppm BA induced embryoid
germination. The small embryogenic clusters cultured in 4℃ 2 days
have less vitality , easier brown than the clusters never cultured
in 4℃.Embryogenesis formation shoot or root , and the time needed
for these develop process in plating clusters is related with the
clusters size and maturation degress of the embryoids and the
composition of the solid medium.