臺灣博碩士論文加值系統

在臨床診斷上約 60%~70% 的肝癌患者血清中Alpha-fetoprotein ( AFP ) 濃度較正常人來的高 ( AFP cut-off 值為 20 mg/ml )，適合作為篩檢肝癌的腫瘤指標蛋白 ( biomarker )。另外在臨床診斷上約50%~80%的大腸癌患者血清中Carcinoembryonic Antigen ( CEA ) 濃度較正常人來的高 ( CEA cut-off 值為 5 ng/ml )，適合作為篩檢大腸癌的 biomarker。然而現行普遍使用的免疫檢測如酵素連結免疫吸附分析 ( Enzyme-Link Immunosorbent Assay；ELISA ) 因其操作過程繁雜與易受待測樣品影響等種種問題，我們需要另尋一種更快速且準確的免疫檢測方法。
本實驗室已歷經使用Anti-CRP( C-reactive protein )與奈米磁性粒子結合，形成一具有磁性生物標記( magnetic biomarker ) 之磁性試劑( magnetic reagent )。再利用本實驗室所研發之磁減量檢測法( Immunomagnetic rednction ; IMR )，其靈敏度可達10-4 mg/ml，於是本研究中我們如法泡製將Anti-AFP 和Anti-CEA 分別與奈米磁性粒子結合成為AFP試劑和CEA試劑，並進行試劑長期穩定度觀察、干擾度測試，最後藉由IMR檢測人類血清檢體，以探討人類肝癌及大腸癌之檢驗特性。

第一章 緒論 .............................................................................................................. 1
第二章 實驗原理
2.1 磁減量檢測 ( Immunomagnetic reduction ; IMR ) 原理介紹 ........... 2

第三章 磁性試劑製程與特性量測
3.1 磁性試劑製程 ....................................................................................... 9
3.2 磁性試劑物理特性量測 ..................................................................... 13
第四章 人類肝癌及大腸癌之磁減量檢驗特性
4.1建立人類肝癌及大腸癌量測標準曲線................................................ 19
4.2使用磁減量檢測 ( Immunmagnetic rednction；IMR ) 與
酵素連結免疫吸附分析 ( Enzyme-Link Immunosorbent Assay；ELISA)
之檢測結果比較………………………………...……….………...…..22
4.3磁減量檢測人類肝癌及大腸癌在臨床之檢驗特性............................ 32
第五章 結論 ............................................................................................................ 35
文獻資料
致謝

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