臺灣博碩士論文加值系統

微藻被為最有潛力的生質能源之一，其相較於其他植物生長快速、佔地面積小和能快速固定二氧化碳。
本研究所使用的藻種是購自東港水產試驗所的擬球藻，並開發新型光合生化反應器來培養藻類，新型光合反應器有良好的曝氣系統和培養循環系統，其培養體積分別為3.5L、20L、100L。
擬球藻所使用的培養基質為Walne’s medium，並在培養中控制其光照、海水鹽度、基質起始酸鹼值和曝氣量。從研究中發現將光照控制在8400Lux、海水鹽度35g/L、起始ph值在7.5和0.25vvm，從結果中得在3.5升新型反應器中培養藻類所表現出效率，遠快於傳統的批處理系統；100L新型反應器效率雖不及於3.5L新型反應器，但其生長效率也是相當快速的，可以作為未來商業運轉的準備。

摘要 I
Abstract III
圖目錄 VII
表目錄 IX
第一章. 緒論 1
1-1前言 1
1-2研究目的 4
第二章.文獻回顧 5
2-1能源 5
2-1-1生質能簡介 7
2-1-2生質柴油簡介 8
2-1-2油脂性能源作物 12
2-2藻類 16
2-2-2培養方式 19
2-2-3影響生長因子 20
2-2-3-1光源和溫度 20
2-2-3-3 pH值 21
2-2-3-4碳源和營養鹽 22
2-2-3-5 氮源 22
2-3培養反應器 23
2-3-1開放式系統 24
2-3-2 封閉式系統 26
第三章. 實驗方法與實驗步驟 31
3-1藻種 31
3-2培養基質 31
3-3實驗設備和實驗藥品 33
3-3-1 實驗相關設備 33
3-3-2實驗相關藥品 33
3-3-3新型光和反應器 34
3-4實驗流程 38
3-5實驗條件 40
3-5-1批次反應器培養策略 40
3-5-2連續循環反應培養策略 40
3-5-3實驗培養條件 41
3-5-3-1光照 41
3-5-3-2通氣量 41
3-5-3-3二氧化碳濃度 42
3-5-3-4氮源 42
3-5-3-5碳源 42
3-6實驗分析方法 43
3-6-1光照強度測定 43
3-6-2藻體生長密度測定 43
3-6-3硝酸鹽分析 43
3-6-4凱式氮檢測法 44
3-6-5 還原糖分析 45
3-6-6含油量 47
3-6-6-1脂肪萃取方法 47
3-6-6-2含油量分析 49
第四章.實驗結果與討論 51
4-1光源影響 51
4-2 通氣量測試 54
4-3鹽度測試 56
4-3-1 不同鹽度在3.5升系統下和20升系統下 56
4-4 二氧化碳濃度 60
4-5直立式藻類培養設備 63
4-6四管直立式反應器 65
4-7以稀酸水解還原糖作為碳源培養 69
4-8油脂萃取 72
第五章.結論與展望 74
附錄 75
參考文獻 78

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