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研究生:黃仁宏
研究生(外文):Huang, Renhung
論文名稱:酸水解紅棗與胖大海多醣的化學組成及其對腸道菌群增生的影響
論文名稱(外文):The Chemical Compositions Of Acid Hydrolyzed Polysaccharides Of Zizyphus Jujube And Sterculia Lychnophora And Their Effect On The Growth Of Internal Microflora
指導教授:王俊權王俊權引用關係
指導教授(外文):Wang, Chiunchuang
口試委員:柯文慶賴麗旭
口試委員(外文):Ko, WenchingLai, Lishiu
口試日期:2012-05-11
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養學系
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:2012
畢業學年度:100
語文別:中文
論文頁數:138
中文關鍵詞:紅棗胖大海多醣抗氧化腸道菌酸水解
外文關鍵詞:Zizyphus jujubaSterculia lychnophorapolysaccharidesantioxidantintestinal microfloraacid hydrolysis
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紅棗( Zizyphus jujuba )與胖大海( Sterculia lychnophora )為台灣常見的中藥材,常利用於藥膳食譜當中。本實驗的目的是探討不同溶液 ( 酸溶液、鹼溶液 & 水 ) 萃取粗多醣,在化學組成、抗氧化力及黏度特性的變化。並將粗多醣進一步經酸水解且利用膠體過濾層析的方法進行分離,以了解不同酸水解程度的紅棗與胖大海多醣之化學組成及結構上的差異,以及其多醣對腸道菌增生的影響。實驗結果顯示,以酸溶液及鹼溶液的粗多醣萃取率較高;利用鹼溶液萃取的紅棗與胖大海多醣具有較高的醣醛酸含量與較佳的抗氧化能力;粗多醣的黏度則以水溶液萃取的紅棗及胖大海多醣最高。在酸水解過後經膠體過濾層析可得到兩個分子量分布,取小分子多醣與未水解多醣進行化學組成、結構與腸道菌增生影響的比較。結果顯示,多醣分子量會隨著水解時間增加而有降低的趨勢;在化學組成比例的變化上,總醣含量皆因經過膠體過濾層析劃分後有顯著提高的趨勢,而醣醛酸含量則隨著水解時間增加而有降低的趨勢;針對腸道菌增生的影響,添加紅棗與胖大海的水解多醣,以水解2 hr及4 hr的小分子多醣濃度1mg / mL時,皆對Bifidobacteriun bifidum有增生的效果,其中以胖大海小分子多醣效果較佳。水解2 hr及4 hr的小分子紅棗多醣對Propionibacterium freudenreichii也有增生的效果,而胖大海則不明顯,推測兩者的差異可能與水解後多醣的醣組成及結構有關。
Zizyphus jujuba ( ZJ ) and Sterculia lychnophora ( SL ) are the traditional Chinese herbs using in the medicated food. The purposes of this study are to investipate the physicochemical properties and antioxidant capacity of polysaccharides of ZJ and SL. Furthermore, the effect of acid hydrolyzed polysaccharides on the growth of internal microflora. The crude polysaccharides were acid hydrolyzed by using diluted hydrochloride solution (2M) for 6hr. The hydrolytes were fractionated by using gel permeation chromatography (GPC) to obtain various molecular weight of polysaccharides. The results indicated that the extraction of acidic and basic solutions obtained higher yield of crude polysaccharides than hot water extraction. The crude polysaccharides from basic solution had the more uronic acid content and the better antioxidant capacity than the others. In the viscosity of crude polysaccharide solutions, water extracts indicated the highest viscosity among three crude polysaccharides in both ZJ and SL. Two major molecular weight distributions were observed as the acid hydrolytes were fractionated by using GPC. The total carbohydrate of hydrolytes was increased after GPC fractionation. However, the content of uronic acid in the acid hydrolyzed polysaccharides was decreased with progress of hydrolysis time. The addition of acid hydrolytes (both 2hr and 4hr) significantly increased the growth of Bifidobacteriun bifidum as the concentration of 1mg / mL was added, particularly in the fraction of low molecular weight of SL. Similarly, the acid hydrolytes (both 2hr and 4hr) of ZJ also significantly increased the growth of propionibacterium freudenreichii, but no significant effect was observed as SL hydrolytes was added. The differences between hydrolytes of SJ and SW could be caused by the different molecular structure of polysaccharide
摘要 I
Abstract II
目錄 IV
表目錄 VII
圖目錄 IX
第一章 前言 1
第二章 文獻回顧 3
ㄧ、紅棗 3
二、胖大海 4
三、多醣 5
四、萃取法 7
五、多醣的純化 ( Purification ) 10
六、劃分 ( Fractionation ) 16
七、多醣的流變特性 19
八、抗氧化能力 21
( 一 ) 自由基與活性氧 21
( 二 ) 抗氧化作用的機制 22
( 三 ) 多醣的抗氧化 24
第三章 材料與方法 31
一、實驗架構 31
二、材料 32
三、粗多醣製備 32
壹、紅棗與胖大海 35
一、一般成分分析 35
(一) 水分測定 35
(一) 粗蛋白測定 35
(二) 粗脂肪測定 35
(三) 灰份測定 35
(四) 粗纖維含量 35
(五) 無氮抽出物 36
貳、粗多醣 36
一、化學組成分析 36
(一) 總醣 (Dubois et al., 1956) 36
(二) 蛋白質 (Smith et al., 1985) 37
(三) 醣醛酸(Blumenkrantz & Asboe-Hansen, 1973) 39
(四) 中性單醣 (Kerherve et al., 1995) 41
二、抗氧化特性 43
(一) DPPH自由基清除能力 43
(二) 還原力 46
(三) 螯合亞鐵離子的能力 47
三、黏度特性 49
叁、酸水解多醣 50
一、化學組成分析 50
(一) 總醣 50
(二) 蛋白質 50
(三) 醣醛酸 50
(四) 中性單醣 50
二、結構和分子量測定 51
(一) 結構分析 51
(二) 分子量測定 52
三、腸道菌群增生的影響 53
肆、統計分析 57
第四章 結果與討論 58
一、基本成分分析 58
二、 多醣化學組成 63
(一) 總醣含量 63
(二)蛋白質含量 63
(三)醣醛酸含量 64
三、 抗氧化特性 70
(一) DPPH自由基清除能力 70
(二) 還原力 74
(三) 亞鐵離子螯合能力 78
四、濃度與溫度對黏度的影響 84
五、酸水解後多醣化學組成 88
(一) 總醣含量 88
(二)蛋白質含量 88
(三)醣醛酸含量 89
(四)中性單醣組成 92
六、多醣結構與分子量 95
(一)分子量大小 95
(二)多醣官能基 96
七、小分子多醣對腸道菌生長的影響 102
第五章、結論 115
參考文獻 116
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