臺灣博碩士論文加值系統

皮膚切片是醫師用來判斷皮膚疾病的準則，在皮膚切片上有分成鑽取活組織檢查(Punch biopsy)、刮削活組織檢查(Shave biopsy)、切口活組織檢查(Incisional biopsy)，皮膚切片檢查是屬於侵入式的檢測方式，這樣的檢測方式會讓病患流血並且可能會留下疤痕，光學同調斷層掃描術(Optical coherence tomography; OCT)在生醫影像中占有很重要的地位，其在量測時不需要做特別的標記，能夠以非侵入的方式量出樣本3D立體影像結構。

在本論文中，利用實驗室生長之摻鈦藍寶石晶體光纖放大自發輻射(Amplified spontaneous emission; ASE)當作光源，架設Mirau-based 全域式同調斷層掃描系統，OCT系統之縱向解析度與光源的中心波長以及頻寬有關。利用兩顆1-W 520 nm之雷射二極體泵浦摻鈦藍寶石晶體光纖可以產生33-mW ASE，其所產生之ASE 中心波長為770 nm，頻寬為164 nm，這樣的光源特性能夠讓Mirau-based 全域式同調斷層掃描系統在空氣中縱向解析度為1.67 μm，在Mirau 物鏡是使用10x水鏡顯微物鏡，其在橫向解析度上為1.65 μm。

這套系統可以用來進行活體皮膚之量測，取得高解析度之3D立體結構，使用近紅外光光源，可以讓活體皮膚量測深度達到約300 μm，這樣高解析度之OCT影像可以用來量化角質層之厚度以及真皮層與表皮層之邊界，得到非侵入式活體皮膚3D影像。

Skin biopsy is the gold standard for doctors to diagnosis skin cancers. There are several ways to do a skin biopsy such as punch biopsy, shave biopsy, incisional biopsy. These methods are all invasive that make the patient bleed and leave scar. Optical coherence tomography (OCT) is one of the most important techniques in biomedical imaging realm. It is a noninvasive, label-free, and 3D imaging method.

In this thesis, a homemade Ti:sapphire crystal fiber amplified spontaneous emission (ASE) light source was used to build a Mirau-based full-field optical coherence tomography system. Using two 520-nm laser diodes to pump the Ti:sapphire crystal fiber, ASE centered at 770 nm with a bandwidth of 164 nm was generated. The Mirau-based full field OCT has an axial resolution of 1.67 μm in air. A 10x mirau objective was employed, and achieved a lateral resolution of 1.65 μm.

Using this system, we can measure in-vivo skin and get the high resolution 3D volume structure. Utilizing the low absorption light source, the penetration depth of in-vivo skin measurement is about 300 μm. We can use this high resolution OCT image to quantitize the thickness of stratum corneum and can distinguish the dermo-epidermal junction.

致謝 I
中文摘要 II
ABSTRACT III
目錄 IV
圖目錄 VI
表目錄 XI
第一章 緒論 1
第二章 光學同調斷層掃描術在皮膚之應用 3
2.1 低同調干涉術 3
2.1.1 縱向解析度 10
2.1.2 橫向解析度 11
2.2 全域式光學同調斷層掃描術 13
2.3 摻鈦藍寶石晶體光纖寬頻光源 15
2.3.1 摻鈦藍寶石晶體光纖製備 15
2.3.2 摻鈦藍寶石晶體光纖放大自發輻射 17
2.3.3 摻鈦藍寶石晶體光纖光源模組 19
2.4 活體皮膚介紹 24
2.4.1 皮膚組織結構 25
2.4.2 皮膚光學特性 28
第三章 Ti:sapphire ASE光源Mirau-based全域式同調干涉系統架構與設計 30
3.1 Mirau 物鏡 30
3.1.1 干涉儀組件設計 33
3.1.2 Mirau物鏡元件 35
3.1.3 膜層設計 38
3.1.4 Mirau 物鏡製作 44
3.2 Mirau-based全域式同調干涉系統 47
3.3 全域式同調干涉系統特性分析 54
3.3.1 縱向解析度 54
3.3.2 橫向解析度 57
3.3.3 訊噪比 60
第四章 Mirau-based同調干涉影像計算與量測 62
4.1 干涉訊號處理 62
4.2 Mirau 全域式光學同調斷層活體皮膚量測影像 66
4.2.1 角質層 68
4.2.2 表皮層 77
4.2.3 表皮層與真皮層交界 79
4.2.4 真皮層 81
4.2.5 血管 83
4.2.6 汗腺 86
4.3 比較Ce:YAG與Ti:sapphire ASE之活體皮膚深度 90
第五章 結論與未來展望 94
參考文獻 96

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