臺灣博碩士論文加值系統

目前常用的DNA偵測方式是利用基材表面鍵結單股DNA捕捉單股互補DNA，而重組DNA微陣列（combinatorial DNA microarrays）為最常用方法之一，但微陣列裝置在製造上是相當耗時且成本較高。相對於微陣列裝置，膠體奈米粒子在製造成本上便宜釵h，因此我們選用膠體奈米粒子溶液替代微陣列裝置。螢光是最常被用來作光學條碼，但同時偵測多個螢光條碼，會有波峰互相重疊情形發生。本研究利用吸附於金奈米粒子的染料分子所產生表面增強拉曼光譜（surface-enhanced Raman scattering，SERS）作為光學條碼。
實驗中利用染料分子X-Rhodamine-5-(and-6)-isothiocyanate（XRITC）當作拉曼標籤，並誘導金奈米粒子聚集成聚集團，再於金聚集團表面修飾一層二氧化矽，作為包覆與保護的作用。最後利用微乳劑法（microemulsion），把合成好的核殼材料表面修飾圓滑的二氧化矽層，使二氧化矽表面能更有效的修飾其他分子，也使核殼材料大小接近一致。本研究除了對整體拉曼標籤溶液做SERS偵測以外，也做單一聚集團的SERS偵測，促使發展單一奈米團的生物感測器。

The common method for DNA detecting is using matrix surface to bind single-stranded DNA and to capture single-stranded complementary DNA. The combinatorial DNA microarray is one of this type of detection, but it costs a lot and is very time-consuming in manufacturing. Comparing colloid nanoparticle with the combinatorial DNA microarrays, we can see the colloid nanoparticle is cheaper in producing. Consequently, we adopt the method of the colloid nanoparticle instead of the method of the combinatorial DNA microarrays. Fluorescence dyes are commonly used as optical barcodes. However, while several fluorescence dyes are detected at same time, the peaks appear overlapped. In this research, we used surface-enhanced Raman scattering (SERS) spectra of dye molecules that absorb gold on nanoparticles as optical barcodes.

We utilized the dye X-Rhodamine-5-(and-6) -isothiocyanate (XRITC) as the Raman marker and the aggregation agent of Au nanoparticle. Then, we capped a silica shell in the Au cluster surface to protect adsorbed dyes. Finally, we apply micoremulsion to produce one smooth and thick silica layer on the core-shell Au cluster, so that the silica surface is more effective for further functionization and Raman markers have the same size. We detect not only SERS spectra of bulk Raman markers, but also SERS spectra of single clusters that could be developed as biosensors.

總目錄
總目錄…………………………………………………………………Ⅳ
圖目錄…………………………………………………………………Ⅵ
中文摘要………………………………………………………………Ⅱ
英文摘要………………………………………………………………Ⅲ
第一章 諸論
1-1. 前言…………………………………………………………… 1.
1-2. 拉曼光譜標記………………………………………………… 2.
1-3. 奈米感測器…………………………………………………… 3.
1-4. 金屬奈米粒子………………………………………………… 7.
1-5. 拉曼光譜學…………………………………………………… 9.
1-6. 表面增強拉曼散射光譜學……………………………………17.
1-7. 微乳液…………………………………………………………25.
1-8. MicroRaman 光譜儀…………………………………………..28.
第二章 實驗儀器架設與實驗步驟
2-1. 實驗儀器架設…………………………………………………35.
2-2. 金奈米粒子合成………………………………………………36.
2-3. 三步驟法包覆拉曼標籤之Au@SiO2奈米團的合成..............38.
2-4. 微乳液法包覆拉曼標籤之Au@SiO2奈米團的合成..............41.
2-5. 表面增強拉曼散射(SERS)光譜的偵測………………………45.
第三章 包覆拉曼標籤之Au@silica奈米團溶液的合成與光譜性質的探討
3-1. 金奈米粒子的合成……………………………………………47.
3-2. 包覆拉曼標籤之Au@ SiO2聚集團的合成………………….50.
3-3. 微乳液法包覆拉曼標籤之Au@SiO2聚集團的合成..............55.
3-4. 包覆拉曼標籤之Au@ SiO2聚集團SERS光譜偵測……….64.
3-5. 包覆拉曼標籤之Au@ SiO2單一聚集團SERS光譜偵測….67.
第四章 結論…………………………………………………………..75.
參考文獻……………………………………………………………….78

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