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研究生:邱慧瑜
研究生(外文):Hui-Yu Chiu
論文名稱:文心蘭花朵香氣及調控異戊二烯生合成相關之DXS基因表現分析
論文名稱(外文):Floral scent analysis in Oncidium orchids and expression of (1-deoxy-D-xylulose-5-phosphate synthase) DXS gene on relation to isoprene biosynthesis
指導教授:陳福旗陳福旗引用關係
指導教授(外文):Fure-Chyi Chen
學位類別:碩士
校院名稱:國立屏東科技大學
系所名稱:農園生產系所
學門:農業科學學門
學類:一般農業學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:中文
中文關鍵詞:文心蘭DXS基因異戊二烯萜類
外文關鍵詞:OnchidiumDXS geneisoterpeneterpeniod
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  • 被引用被引用:2
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  • 下載下載:40
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萜類為植物二次代謝物的最大家族,可調控植物生長發育、吸引昆蟲傳播媒介進行花朵受粉及作為植物防禦病蟲害侵入。植物香氣由單萜類及倍半萜類所組成,在質體內經由異戊二烯路徑所產生。本研究探討異戊二烯上游的DXS基因與文心蘭香味之關係。經RT-PCR分析,香水文心蘭 (Oncidium Sharry Baby ‘Sweet Fragrance’) 全開花於上午十點的DXS基因表現量高於其它組織部位;用GC-MC分析全開花,也是以上午十點比其他時間的相對含量最高,香味成份中已鑑定出α-pinene、3-carene、linalool、limonene、1,8-cineole、β-ocimen、linalyl acetate、p-menth-1,4 (8)-diene、menthatriene、quinoline與caryophyllene,以3-carene的相對含量較其他化合物高。對照品種Onc. Gower Ramsey含較多的倍半萜類。綜合以上結果顯示DXS基因的表現量與香氣釋放量有正相關。為瞭解花朵中的表現標簽序列(expressed sequence tag, ESTs)與香氣產生是否同樣具有日夜差異,利用全開花分離之cDNA進行扣除雜交分析,初步得到45個上午十點及45個凌晨兩點之全開花的特異性ESTs,其中包含不同功能的ESTs,另表現量差異最為顯著的有Cymbidium mosaic virus (CymMV)病毒基因序列,其次為功能未知蛋白、代謝相關基因、訊息傳遞相關基因、細胞生長與分裂及蛋白質合成等基因;以RT-PCR檢測後,凌晨兩點全開花之CymMV病毒基因表現量皆高於上午十點,推測CymMV病毒基因在白天的活性較晚上低,此結果需更進一步驗證。
中文摘要 I
ABSTRACT III
誌謝 V
目錄 VI
圖表目錄 VIII
壹、前言 1
貳、前人研究 2
一、文心蘭的介紹與分類地位 2
二、植物香氣來源及功能 3
三、植物萜類生合成路徑 4
四、DXS基因功能性分析 6
五、萜類合成酶基因(Terpene synthase gene) 7
參、材料與方法 9
一、試驗材料 9
二、試驗方法 9
(一) 總量RNA萃取 9
(二) 文心蘭DXS基因表現量分析-反轉錄聚合酶連鎖反應(RT-PCR) 10
(三) 選殖DXS基因全長cDNA 11
(四) 文心蘭DXS基因啟動子選殖 13
(五) cDNA扣除雜交分析 17
(六) 文心蘭病毒檢測 22
(七) 文心蘭花朵GC-MS分析 23
肆、結果 25
一、文心蘭DXS基因序列之分析 25
二、三種文心蘭DXS基因之表現量分析 26
三、文心蘭DXS啟動子選殖及調控序列分析 27
四、cDNA扣除雜交之ESTs library建構 28
五、文心蘭之病毒檢測 29
六、香氣分析及成份鑑定 29
伍、討論 31
一、文心蘭DXS基因之表現量 31
二、文心蘭DXS啟動子之調控 32
陸、結論 34
柒、參考文獻 83
作者簡介 92


蔡佩芬、李哖 (2001) 巧克力文心蘭花需涼溫。台灣花卉園藝168: 34-39。
林瑞松 (2002) 文心蘭栽培技術。文心蘭專刊。財團法人台灣區花卉發展協會。pp. 54-64。
易美秀 (2000) 文心蘭授粉、結實與無菌播種之研究。國立中興大學園藝系碩士論文。
謝清祥 (2002) 台灣文心蘭早期栽培簡介。文心蘭專刊。財團法人台灣區花卉發展協會。pp. 42-53。
賴本智(2002) 文心蘭、蜘蛛蘭、堇花蘭、齒舌蘭琪近原屬的種源介紹。文心蘭專刊。財團法人台灣區花卉發展協會。pp. 86-133。
易美秀 (2003) 文心蘭雜交育種。台中區農情月刊。47: 102-115。
邱金春 (2002)常見的文心蘭盆花品種。文心蘭專刊。財團法人台灣區花卉發展協會。pp. 134-140。
王瑞章、孫文章、胡文若、陳俊仁、江汶錦 (2006) 栽培介質對盆栽文心蘭生育與開花品質之影響。台南區農業改良場研究彙報。47: 9-16。
邱金春 (2010) 遠源雜交在文心蘭育種之應用。台灣花卉園藝。269: 21-25。
陳麗琦 (2009) 控制文心蘭花瓣表皮細胞相關MYB基因表現之分析。國立屏東科技大學農園生產系碩士論文。
陳雅惠 (2004) 蝴蝶蘭三唇瓣突變種表現基因之分析。國立屏東科技大學熱帶農業暨國際合作研究所碩士論文。
溫佩容 (2007) 文心蘭花朵有色體專一性蛋白基因及啟動子表現之研究。國立屏東科技大學農園生產系碩士論文。

Aharoni, A., M. A. Jongsma, and H. J. Bouwmeester (2005) Volatile science? Metabolic engineering of terpenoids in plants. Trends Plant Sci. 10: 594-602.
Aubourg, S., A. Lecharny, and J. Bohlmann (2002) Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana. Mol. Genet. Genomics 267: 730-745.
Bergougnoux, V., J. C. Caissard, F. Jullien, J. L. Magnard, G. Scalliet, J. M. Cock, P. Hugueney, and S. Baudino (2007) Both the abaxial and abaxial epidermal layers of the rose petal emit volatile sent compounds. Planta 226: 853-866.
Bohlmann, J., and R. Croteau (1999) Inscent-plant interactions and indurced plant defence. Diversity and variability of terpenoid defences in conifers: molecular genetics, biochemistry and evolution of the terpene synthase gene family in grand fir (Abies grandis) Wiley. Chichester 223: 132-149.
Botella-Pavia, P., O. Besumves, M. A. Phillips, C. P. Lorenzo, A. Bornat and R. C. Manuel (2004) Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethlbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors. Plant J. 40: 188-199.
Busk, P. K and M. Pages (1998) Regulation of abscisic acid-induced reanscription. Plant Mol. Biol. 51: 313-325.
Carretero-Paulet, L., A. Cairó, P. Botella-Pavía, O. Besumbes, N. Campos, A. Boronat and M. Rodríguez-Concepción (2006) Enhanced flux through the methylerythritol 4-phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase. Plant Mol. Biol. 62:683-695.
Chen, F., D. K. Ro, J. Petri, J. Gershenzon, J. Bohlmann, E. Pichersky and D. Tholl (2004) Characterization of a root-specific Arabidopsis terpene sunthase responsible for the formation of the volatile monoterpene 1,8-cineole. Plant Physiol. 135:1956-1966.
Chen, F., D. Tholl, J. C. D’Auria, A. Farooq, E. Pichersky, and J. Gershenzon (2003) Biosynthesis and emission of terpenoid volatiles from Arabidopsis flower. Plant Cell 15: 481-494.
Chen, K., Y. K. Yauk, N. J. Nieuwenhuizen, A. J. Matich, M. Y. Wang, R. L. Perez, R. G. Atkinson, and L. L. Beuning (2010) Characterisation of an (S)-linalool synthase from kiwifruit (Actinidia arguta) that catalyses the first committed step in the production of floral lilac compounds. Funct. Plant Biol. 37: 232-243.
Combrinck, S., G. W. Du Plooy., R. I. McCrindle and B. M. Botha (2007) Morphology and histochemistry of the glandular trichomes of Lippia scaberrima (Verbenaceae). Ann. Bot. 99: 1111-1119.
Dudareva, E., and E. Pichersky (2000) Biochemical and molecular gentic aspects of floral scents. Plat Physiol. 122: 627-633.
Dudareva, N. and E. Pichersky (2006) Biology of Floral Scent. CRC Press, Boca Raton. pp.105-116.
Dudareva, N. D. Martin, C. M. Kish, N. Kolosova, N. Gorentein, J. Faldt, B. Miller and J. Bohlmann (2003) (E)-β-Ocimene and mycene synthase genes of floral scent biosynthesis on snapdragon: function and expression of three terpene synthase gens of a new terpene synthase subfamily. Plant Cell 15:1227-1241.
Dudareva, N., L. Cseke, V. M. Blanc and E. Pichersky (1996) Evolution of floral scent in Clarkia: novel pattems of S-linalool synthase gene expression in the C. breweri flower. Plant Cell 8: 1137-1148.
Dudareva, N., S. Andersson, I. Orlova, N. Gatto, M. Reichelt, D. Rhodes, W. Boland and J. Gershenzon (2005) The nonmevalonate pathway supports both monterpene and sesquuterpene formation in snapdragon flowers. Plant Biol. 102: 933-938.
Dun, E. A., P. B. Brewer and C. A. Beveridge (2009) Strigolactones: discovery of the elusive shoot branching hormone. Trends Plant Sci. 14: 364-372.
Estevez, J. M., A. Cantero, A. Reindl, S. Reichler, and P. Leon (2001)1-deoxy-D-xylulose-5-phoshate synthase, a limiting enzyme for platidic isoprenoid biosynthesis in plant. J. Biol. Chem. 276: 901-909.
Estevez, J. M., A. Cantero., C. Romero, H. Kawaide, L. F. Jimenez., T. Kuzuyama., Haruo. Seto., Y. Kamiya and P. Leon (2000) Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5-phosphatesynthase of the 2-C-methyl-d-erythritol-4-phosphate pathway in Arabidopsis. Plant Physiol. 124: 95-103.
Gomez-Roldan, V., S. Fermas, P. B Brewer, V. Puech-pages, E. A. Dun, J. P. Pillot, F. Letisse, R. Matusova, S. Damoun, J. C. Portais, H. Bouwmeester, G. Becard, C. A. Beveridge, C Rameau and S. F. Rochange (2008) Strigolactone inhibition of shoot branching. Nature 455: 189-194.
Guterman, I., M. Shalit, N. Menda, D. Piestun, M. Dafny-Yelin, G. Shalev E. Bar, O. Davydov, M. Ovadis, M. Emanuel, J. Wang, Z. Adam, E. Pichersky, E. Lewinsohn, D. Zamir, A. Vainstein and D. Weiss (2002) Rose scent: genomics approach to discovering novel floral fragrance-related genes. Plant Cell 14: 2325-2338.
Helsper, J. P. F. G., J. A. Davies, H. J. Bouwmeester, A. F. Krol, and M. H. Van Kampen (1998) Circadian rhythmicity in emission of volatile compounds by flowers of Rosa hybrida L. cv.honesty. Planta 207:88-95.
Hiratsu, K., O.T. Masaru, S. Kazuo and Y. S. Kazuko (2005) AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17: 3470–3488.
Hsieh, M. H., and H. M. Goodman (2005) The Arabidopsis IspH homology is involved in the plastid nonmevalonate pathway of isopernoid biosynthesis. Plant Physiol. 138: 641-653.
Huang, D., W. Wu, S. R. Abrams and A. J. Cutler (2008) The relationship of drought-related gene expression in Arbidopsis thaliana to hormonal and environmental factors. J. Exp. Bot. 59: 2991-3007.
Ibrahim, M. A., M. Maenpaa, V. Hassinen, S. Kontunen-Soppela, L. Malec, M. Rousi, L. Pietikainen, A. Tervahauta, S. Karenlampi, J. K. Holopainen and E. J. Oksanen (2010) Elevation of night-time temperature increases terpenoid emissions from Betula pendula and Populus tremula. J. Exp. Bot. 61: 1583-1595.
Kolosova, N., D. Sherman, D. Karlson and N. Dudareva (2001) Cellular and subcellular localization of S-adenosyl-L-methionine: benzoic acid carboxyl methyltransferase, the enzyme responsible for biosynthesis of the volatile ester methylbenzoate in snapdragon flowers. Plant Physiol. 126: 956-964.
Lane, A., A. Boecklemann, G. N. Woronuk, L. Sarker and S. S. Mahmoud (2010) A genomics resource for investigating regulation of essential oil production in Lavandula angustifolia. Plant 231:835-845.
Lavoir1,A. V., M. Staudt1, J. P. Schnitzler, D. Landais1, F. Massol, A. Rocheteau, R. Rodriguez1, I. Zimmer, and S. Rambal (2009) Drought reduced monoterpene emissions from the evergreen Mediterranean oak Quercus ilex: results from a through fall displacement experiment. Biogeosciences 6:1167–1180.
Ledford and Niyogi (2005) Singlet oxygen and photo-oxidative stress management in plants and algae. Plant Cell Environ. 28: 1037-1045.
Loretol, F., A. Forster, M. Durr, O. Csiky and G. Seufert (1998) On the monoterpene emission under heat stress and on the increased thermotolerance of leaves of Quercus ilex L. fumigated with selected monoterpenes. Plant Cell Environ. 21: 101–107.
Luisa, M. L., R. C. Manuel, F. Gallego, N. Campos and A. Boronat (2000) Carotenoid biosynthesis during fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase. Plant J. 22: 503-513.
Martin, D. M., J. Gershenzon and J. Bohlmann (2003) Induction of volatile terpene biosynthesis and diurnal emission by methyl jasmonate in foliage of Norway Spruce. Plant Physiol. 132: 1586-1599.
Martin, D., D. Tholl, J. Gershenzon and J. Bohmann (2002) Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosynthesis, and terpenoid accumulation in developing xylem of Norway Spruce stems. Plant Physiol. 129: 1003-1018.
Martinez-Garocia, J. F., E. Huq, and H. Quail (2000) Direct targeting of light signals to a promoter element-bound transcription factor. Science 288: 859-863.
McConkey, M. E., J. Gershenzon, and R. B. Croteau (2000) Developmental regulation of monoterpen biosynthesis in the glandular trichomes of peppermint. Plant Physiol. 122: 215-223.
Munoz-Bertomeu, J., I. Arrillaga, R. Ros and J. Segura (2006) Up-regulation of 1-deoxy-d-xylulose-5 phosphate synthase enhances production of essential oils in transgenic spi;e lavender. Plant Physiol. 142: 890-900.
Nieuwenhuizen, N. J., M. Y. Wang, A. J. Matich, S. A. Green. X. Chen, Y. K. Yauk, L. L. Beuning, D. A. Nagegowda, N. Dudareva and R. G. Atkinson (2009) Two terpene synthases are responsible for the major sesquiterpenes emitted from the flowers of kiwifruit (Actinidia delicisa). J. Exp. Bot. 10:1-17.
Owen, S. M, and J. Penuelas (2005) Opportunistic emissions of volatile isoprenoids. Trends Plant Sci. 10:420-426.
Patrica, B. P., O. Besumves, M. A. Phillips, C. P. Lorenzo, A. Boronat and R. C. Manuel (2004) Regulation of cartenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reduvtase in controlling the supply of plastidial isoprenoid precursors. Plant J. 40: 188-199.
Peter, A. J and T. G. Shanower (1998) Plant glandular trichomes. Resonance 3: 41-45.
Pichersky, E., and J. Gershenzon (2002) The formation and function of plant volatiles: perfumes for pollinator attraction and defense. Curr. Opin. Plant Biol. 5: 237-247.
Pichersky, E., J. P. Noel and N. Dudareva (2006a) Biosynthesis of plant volatiles: nature’s diversity and lngenuity. Scince 311: 808-811.
Pichersky, E., and N. Dudareva (2006b) Scent engineering: toward the goal of controlling how flowers smell. Trends Biotechnol. 25:105-110.
Pridgeon, A and A. Morrison (1992) The Illustrated Encyclopedia of Orchids. Timer Press, Portland, Oregon. pp. 192-204.
Rodríguez-Concepción, M., I. Ahumada, E. Diez-Juez, S. Sauret-Gȕrto, L. M. Loist, F. Gallego, L. Carretero-Paulet., N. Campos and A. Boronant (2001) 1-deoxy-d-xylulose-5 phosphate reductiosomerase and plastid isopernoid biosynthesis during tomato fruit ripening. Plant J. 27: 213-222.
Rohmer, M. (1999) The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algel and higher plants. Nat. Prod. Report. 16:565-574.
Seo, M and T. Koshiba (2002) Complex regulation of ABA biosynthesis in plant. Trends plant Sci. 7: 41-47.
Serna, L. and C. Martin (2006) Trichomes : different regulatory network lead to convergent structures. Trends Plant Sci. 11: 274-280.
Tingey, D., M. Manning, L. C. Grothaus and W. F. Burns (1980) Influence of light and temperature on monoterpene emission rates from Slash Pine. Plant Physiol. 65: 797-801.
Umerhara, M., A. Hanada, S. Yoshida, K. Akiyama, T. Arite, N. Takeda-kamiya, H. Magome, Y. Kamiya, K. Shirasu, K. Yoneyama, J. Kyozuka and S. Yamaguchi (2008) Inhibition of shoot branching by new terpenoid plant hormones. Nature 455: 195-200.
Valterova, I., J. Kunze, A. Gumbert, A. Luxova, I. Liblikas, B. Kalinova, and A. K. Borg-Karlson (2007) Male bumble bee pheromonal components in the scent of deceit pollinated orchids; unrecognized pollinator cues? Arthropod Plant Interact. 1: 137-145.
Van Schie, C. C. N., M. A. Haring, and R. C. Schuurink (2006) Regulation of terpenoid and benzenoid production in flowers. Curr. Opin. Plant Biol. 9: 203-208.
Werker, E. (2000) Trichome diversity and development. Adv. Bot. Res. 31: 1-35.
Wiemer. A. P., M. More, S. Benitz-Vieyra, A. A. Cocucci, R. A. Raguso and A. N. Sersic. (2009) A simple floral fragrance and unusual osmophore structure in Cyclopogon elatus (Orchidaceae). Plant Biol. 11: 506-514.
Withers, S. T and J. Keasling (2007) Biosynthesis and engineering of isoprenoid small molecules. Appl. Microbiol. Biotechnol. 73: 980-990.
Zulak, K. G and J. Bohlmann (2010) Terpenoid biosynthesis and specialized vascular cells of conifer defense. J. Integr Plant Biol. 52: 86-97.

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