臺灣博碩士論文加值系統

本研究的目的主要探討乳酸菌、蔬果水解物和黑麥汁對大鼠腸道菌相和血清中抗氧化能力的影響，研究將分為兩部分進行。實驗一利用60隻六週齡雄性Sprague-Dawley大白鼠，並分別或合併給予乳酸菌和蔬果水解物，隨機分成六組，分別為控制組(Control)、Group A (乳酸菌3.60×107 CFU) 、Group B(乳酸菌3.60×108 CFU)、Group C (乳酸菌7.20×108 CFU)、Group D(乳酸菌3.60×107 CFU, 蔬果水解物178mg/kg/day)、Group E(蔬果水解物178mg/kg/day)，分別於飼養四週，八週後進行直腸糞便總厭氧菌、乳酸桿菌、雙叉桿菌、大腸桿菌以及產氣莢膜梭菌等菌數之分析並觀察分析血清中Catalase、SOD、GSH、GPX抗氧化能力。結果顯示，飼養第四週、第八週時，Group A、Group B、Group C、Group D、Group E與控制組相比，糞便和盲腸中總厭氧菌數、乳酸桿菌數和雙叉桿菌數皆有增加(p＜0.05)的現象，糞便和盲腸中大腸桿菌數和產氣莢膜梭菌數皆有下降(p＜0.05)的現象，其血清抗氧化能力方面，Group A、Group B、Group C、Group D、Group E與控制組相比，Catalase、GSH、GPX氧化能力皆有增加(p＜0.05) 情形，Group B、Group C、Group D、Group E與控制組相比，SOD氧化能力皆有增加(p＜0.05) 情形。實驗二利用50隻六週齡雄性Sprague-Dawley大白鼠，並給予黑麥汁萃取物，隨機分成五組，分別為控制組(Control)、Group A ( 4.12 g /kg bw/day)、Group B ( 8.23 g/kg bw/day) 、Group C (16.26 g/kg bw/day)以及Group D (41.16 g/kg bw/day)，分別於飼養二週，四週，八週後進行直腸糞便總厭氧菌、乳酸桿菌、雙叉桿菌、大腸桿菌以及產氣莢膜梭菌等菌數之分析。結果顯示，在飼養第二週時，Group A、Group B、Group C、Group D與控制組相比，糞便中總厭氧菌數、乳酸桿菌、雙叉桿菌數皆有增加(p＜0.05)的現象，Group B、Group C、Group D與控制組相比，糞便中大腸桿菌數有下降(p＜0.05)的現象；飼養第四週、第八週時，Group A、Group B、Group C、Group D與控制組相比，糞便和盲腸中總厭氧菌數、乳酸桿菌數和雙叉桿菌數皆有增加(p＜0.05)的現象，糞便和盲腸中大腸桿菌數和產氣莢膜梭菌數皆有下降(p＜0.05)的現象；)。因此，分別或合併給予乳酸菌和蔬果水解物可以增加大鼠血清中抗氧化能力並改善腸道菌相；補充給予黑麥汁則可以改善大鼠腸道菌相。由此可以得知對於補充給予乳酸菌、蔬果水解物、黑麥汁可以經由改善腸道菌相來維持腸胃道功能之正常。

This study was divided into two parts: The aim of first part study,was evaluate the effect of lactic acid bacteria and/or fruits vegetable fermentant in rat intestinal microflora and serum antioxidant capacity.Sixty Sprague-Dawley rats were randomly divided into six groups : control group, group A (lactic acid bacteria 3.60 × 107 CFU), group B (lactic acid bacteria 3.60 × 108 CFU), group C (lactic acid bacteria 7.20 × 108 CFU), group D (lactic acid bacteria 3.60 × 107 CFU, fruits and vegetables hydrolyzate 178mg/kg/day), group E (fruits and vegetables hydrolysates 178mg/kg/day), fecal samples were collected at weeks 4 and 8 for intestinal microflora determination.The serum Catalase, SOD, GSH, GPX were examinated for antioxidant capacity.The results showed that total anaerobes, bifidobacteria, lactobacilli counts increased in group A, B, C, D and E when compared with control group at the 4 week period and 8 week period in the fecal and cecum.The E.coli and Clostridium perfringens counts decreased in group A, B, C, D and E when compard with control group at the 4 week period and 8 week period in the fecal and cecum. The Catalase, SOD, GSH antioxidant capacity increased in group A, B, C, D and E when compared with the control group. GPX antioxidant capacity increased in group B, C, D and E when compared with control group. The aim of second part study, was to evaluate the effect of black wheat juice extract on rat intestinal microflora. Fifty Sprague-Dawley rats were randomly divided into five groups : control group, group A (4.12 g/kg bw /day), group B (8.23 g/kg bw /day), group C (16.26 g/ kg bw/day) and group D (41.16 g/ kg bw/day).Fecal samples were collected at weeks 2, 4 and 8 for intestinal microflora determination.The results showed that total anaerobes, bifidobacteria, lactobacilli counts increased in group A, B, C and D when compared with control group at the 2 week period, 4 week period and 8 week period in the fecal and cecum.The E.coli counts decreased in group B, C and D when compared with control group at 2 week period on the fecal, the E.coli amd Clostridium perfringens counts decreased in group A, B, C and D when compared with control group at the 4 week period and 8 week period. Therefore, lactic acid bacteria and/or fruits vegetable fermentant can increase the serum antioxidant capacity and improve the intestinal microflora.Black wheat juice also can improve the intestinal microflora.Lactic acid bacteria, fruits vegetable fermentant and black wheat juice can improve the intestinal microflora through to maintain normal bowel function.

誌謝…………………………………………………………………………….…….. ii
摘要………………………………………………………………………...…............ iii
第壹章 前言及文獻回顧…………………………………………………………... 1
一、 前言……………………………………………………………….….. 1
二、 腸道菌相………………………………………………………….….. 3
三、 益生菌………………………………………………………………... 7
四、 益生質………………………………………………………………. 17
第貳章 研究目的……………………………………………………..…………... 22
第叁章 實驗設計…………………………………………………………………. 23
第肆章 材料與方法………………………………………………………………. 25
第伍章 結果……………………………………………………………..………... 50
第陸章 討論……………………………………………………………..………... 62
第柒章 參考文獻…..…………………………………………………..…………. 66

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