臺灣博碩士論文加值系統

中 文 摘 要
本試驗旨在自豬肝臟萃製高蛋白質含量之粉末，並藉酵素水解增加其在水中的溶解度及還原三價鐵(Fe(Ⅲ))離子能力，進而提高人體對非血質鐵質的吸收為目的。
自市場購得之新鮮豬肝臟，於4℃下儲存備用，分全肝臟、水溶、鹽溶與不溶性肝臟等樣品，再經酵素處理與冷凍乾燥後，將所得之各樣品分析其一般成分、各成分之收率、在不同pH值下相對溶解度與對Fe (Ⅲ)之還原能力。結果顯示，水分部分，以新鮮肝臟（FL）為最高（P＜0.05）；灰分部分，以乾燥後全肝粉（DWFL）及經酵素水解之乾燥全肝粉（DDWFL）含量最高（P＜0.05）；粗脂肪以DWFL部分為最高（P＜0.05）；蛋白質部分以WPF與DWPF含量最高（P＜0.05）。
肝臟各樣品成分收率，以DWFL與DDWFL含量最高；經酵素水解後DWFP與鹽溶性肝粉(DSPF)兩部分則略增，經酵素水解之不溶性肝粉(DIPF)則略降。
當各部分之相對溶解度與WPF或DWPF於pH3.0者相比較時，介於pH4.0∼pH5.0時，各部份皆低於20%； 在pH6.0∼pH10.0間，則以WPF、鹽溶性肝粉（SPF）、DWPF與DSPF四部份相當，皆在75%左右。
酵素水解可提高肝粉在水溶液中的相對溶解度，水解後各部分肝粉其相對溶解度均較未水解前提高約40-50％；其中以DWPF部分除在pH4.0與pH5.0其相對溶解度約在60%外，其餘皆高於120%為最佳。
對Fe (Ⅲ)之還原能力量部分，未經酵素處理者，以WPF最佳，當添加量達0.05﹪時，則還原力已趨飽和；經酵素處理後則以DWPF之Fe (Ⅲ)還原量最高，其添加量須達0.1時，其還原力方趨飽和。

英 文 摘 要
Reductive Ability of Porcine Liver and its
Digestive Peptide Powder on Non-heme Iron.
Wen-Chuan Chu
Abstract
The purpose of this study was to manufacture high protein powder from porcine liver by enzyme digesting to increase its solubility in water and the reductive ability of ferric iron toim prove the absorption of non-heme iron in human body.
The fresh porcine liver samples were bought from traditional market and stored at 4℃. The samples were divided into whole fresh liver(FL), water-soluble, salt-soluble and insoluble fractions followed without or with enzyme digestion, then those frozen dried power were analyzed the general composition, the weight of percentage, the relative solubility in different pH-value and the reductive ability of ferric iron. In result, FL was the highest in moisture(P＜0.05). Dry whole fresh liver(DWFL) and digested dry whole fresh liver(DDWFL) were the highest in ash(P＜0.05). DWFL was the highest in crude fat(P＜0.05). Water-soluble fraction(WPF) and digested water-soluble fraction(DWPF) were the highest in crude protein(P＜0.05). DWFL and DDWFL have the highest percentage of dry weight, but DWPF and digested salt- soluble fraction(DDSFL) were slightly increased and digested insoluble fraction(DIFL) decreased slightly.
When compared the relative solubility of each group with WPF or DWPF at pH3.0, all fractions were below 20% from pH4.0-5.0. The relative solubility of WPF, SPF, DWPF and DSPF were equal around 75% in pH6.0-10.0.
Each fraction of porcine liver powder treated with enzyme could raise the relative solubility about 40-50% than that without enzyme digested . The relative solubility of DWPF at pH4.0-5.0 were around 60% and other pH were over 120%.
The fraction without enzyme treatment, WPF had the highest ferric iron reductive ability on the concentration at 0.05%, and DWPF needed to reach 0.1%. The ferrous iron reductive ability between WPF at 0.05% and DWPF at 0.1% were no significant difference(P＞0.05).

目次 頁次
壹、摘要 1
貳、緒言 3
參、文獻檢討 4
肆、材料與方法 41
伍、結果與討論 46
陸、結論 65
柒、參考文獻 67
捌、英文摘要 80
玖、小傳 83

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