跳到主要內容

臺灣博碩士論文加值系統

(216.73.217.75) 您好!臺灣時間:2026/08/21 02:01
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:徐思維
研究生(外文):Ssu-Wei Hsu
論文名稱:鐵炮百合花藥發育和花粉萌發生長的三個專一性基因之分子調控分析
論文名稱(外文):The analysis for molecular regulation of three specific genes involved in the lily anther development and pollen germination and tube growth
指導教授:王國祥
學位類別:博士
校院名稱:國立中興大學
系所名稱:生物科技學研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:2010
畢業學年度:98
語文別:英文
論文頁數:124
中文關鍵詞:乾燥離層酸花粉
外文關鍵詞:DesiccationAbscisic acidPollen
相關次數:
  • 被引用被引用:0
  • 點閱點閱:205
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
在百合花粉成熟期和花粉管生長期間,Rop基因會受乾燥和離層酸訊息傳遞調控,當把未成熟的花藥/花粉進行乾燥處理和外加不同濃度的離層酸處理時,LLP-Rop1基因會降低其mRNA的累積,而LLP12-2,一個Rop-interactive CRIB motif-containing (RIC)基因則會增加其mRNA的累積;利用離層酸合成抑制劑norflurazon處理,也顯示離層酸會抑制LLP-Rop1,且會誘導LLP12-2基因表現。LLP-Rop1大量表現時,會抑制花粉管延長和造成管尖膨大的現象,CFP-LLP-Rop1分布在花粉管尖端的原生質膜上,Fluorescence resonance energy transfer (FRET)分析顯示,在花粉管中,活化的LLP-Rop1會和LLP12-2發生交互作用,但此交互作用可能不會受花粉乾燥的影響。當LLP12-2大量表現時,會抑制花粉萌發和花粉管生長,利用鈣離子流入過程的抑制劑LaCl3和EGTA及actin去極化藥物LatB處理,顯示LLP12-2會調控鈣離子流入的過程,而與actin的聚集無關。進一步以蛋白激&;#37238;抑制劑staurosporine或蛋白磷酸&;#37238;抑制劑okadaic acid處理,顯示LLP12-2調控鈣離子流入的過程與對staurosporine敏感的蛋白激&;#37238;和對okadaic acid敏感的蛋白磷酸&;#37238;無關。更進一步利用LLP12-2突變體來探討LLP12-2蛋白質的功能,顯示LLP12-2蛋白的N端扮演effector domain的功能而影響花粉萌發和花粉管生長

Here, we report unique desiccation-associated abscisic acid (ABA) signaling transduction through which the Rop (Rho GTPase of plants) gene is regulated during the stage of pollen maturation. Accumulation of the LLP-Rop1 transcript decreased its level of accumulation while LLP-12-2, a Rop-interactive CRIB motif-containing (RIC) transcript increased either by premature drying of developing anther/pollen or by the exogenous application of various concentrations of ABA during pollen maturation and tube growth. Application of norflurazon, an ABA biosynthesis inhibitor, also resulted in the downregulation of the LLP-Rop1 gene while the LLP-12-2 was upregulated by ABA. LLP-Rop1 overexpression inhibited tube elongation, caused tube expansion and the formation of a ballooned tip. CFP-LLP-Rop1 was localized to the cytoplasm having a greater intensity along the tube plasma membrane. Fluorescence resonance energy transfer (FRET) analysis of lily pollen tubes coexpressing CFP-LLP-Rop1 and YFP-LLP-12-2 demonstrated that LLP-12-2 is a target RIC protein of active LLP-Rop1, but the interaction between LLP-Rop1 and LLP-12-2 proteins is probably irrelevant of dehydration in the dried pollen. Overexpression of LLP12-2 inhibits pollen germination and tube growth. Studies with the germination inhibitors, the Ca2+ influx blocking agents LaCl3 and EGTA and an actin-depolymerizing drug, latrunculin B (LatB), suggested that LLP12-2 is involved in modulating calcium influx in the cytoplasm, but not actin assembly. When pollen germination and tube elongation were further examined by LaCl3 or EGTA, each with the addition of either staurosporine or okadaic acid, the results revealed that the involvement of LLP12-2 in modulation of calcium influx in the cytoplasm is independent of staurosporine-sensitive protein kinases and okadaic acid-sensitive phosphatases. The deletion mutant analysis of LLP12-2 indicated that the N-terminal region of LLP12-2 acts as an effector domain which regulates pollen germination and tube growth.

TABLE OF CONTENTS

Chapter I: Anther Development
INTRODUCTION………………………………………………………..2
REFERENCES………………………………………………………….9

Chapter II: Rop GTPase and Its Target Cdc42/Rac-Interactive-Binding Motif-Containing Protein Genes Respond to Desiccation and Involve Calcium Influx likely Independent of Phosphoprotein Regulation during Pollen Maturation, Germination and Tube Growth

ABSTRACT (ENGLISH)……………………………………………….12
ABSTRACT (CHINESE)……………………………………………….14
INTRODUCTION………………………………………………………15
MATERIALS AND METHODS………………………………………..22
RESULTS……………………………………………………………….33
DISCUSSION…………………………………………………………...44
REFERENCES………………………………………………………….52
TABLES and FIGURES………………………………………………...61
APPENDIX……………………………………………………………….82

Chapter III: Modulation of Lily Anther-Specific PR10 Gene through Gibberellin, Abscisic Aacid, and Ethylene and Their Crosstalk

ABSTRACT (ENGLISH)………………………………………….……..83
ABSTRACT (CHINESE)………………………………………………...84
INTRODUCTION………………………………………………………..85
MATERIALS AND METHODS…………………………………………91
RESULTS……………………………………………………………….95
DISCUSSION…………………………………………………………...101
REFERENCES………………………………………………………….104
FIGURES……………………………………………………………….112


胡適宜。1991。被子植物之胚胎學,曉園出版社,55-56頁。
許圳塗、金石文、阮明淑。2002。實用花卉栽培技術專輯5-百合。財團法人台灣區花卉發展協會。
蔡月夏。1995。台灣農家要覽農作篇(二),財團法人豐年社主編,585-588頁。
Allwood, E. G., Anthony, R. G., Smertenko, A. P., Reichelt, S., Drobak, B. K., Doonan, J. H., Weeds, A. G., and Hussey, P.J. (2002) Regulation of the pollen-specific actin-depolymerizing factor LlADF1. Plant Cell 14: 2915-2927.
Balsamo, R. A., Wang, J. L., Eckard, K.J. Wang, C.-S., and Lord, E.M. (1995) Immunogold localization of a developmentally regulated, tapetal-specific, 15 kDa lily anther protein. Protoplasma 189: 17-25.
Becker, J. D., Boavida, L. C., Carneiro, J., Haury, M., and Feijo, J. A. (2003) Transcriptional profiling of Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant Physiol. 133: 713-725.
Bedinger, P. A. (1992) The remarkable biology of pollen. Plant Cell 4: 879-887.
Blomstedt, C. K., Knox, R. B., and Singh, M.B. (1996) Generative cells of Lilium longiflorum posses translatable mRNA and functional protein synthesis machinery. Plant Mol. Biol. 31: 1083-1086.
Buitink, J., Leprince, O., Hemminga, M.A., and Hoekstra, F.A. (2000) The effects of moisture and temperature on the ageing kinetics of pollen: interpretation based on cytoplasmic mobility. Plant Cell Environ. 23: 967-947.
Chiang, J.-Y., Shu, S.-W., Ko, C.-W., and Wang, C.-S. (2006) Biochemical characterization of a pollen-specific cDNA encoding polygalacturonase in Lilium longiflorum. Plant Sci. 170: 433-440.
Crossley, S. J., Greenland, A. J., and Dickinsion, H. G. (1995) The characterization of tapetum-specific cDNAs isolated from a Lilium henryi L. meiocyte subtractive cDNA library. Planta 196: 523-529.
Eady, C., Lindsey, K., and Twell, D. (1995) The significance of microspore division symmetry for vegetative cell-specific transcription and generative cell differentiation. Plant Cell 7: 65-74.
Edlund, A. F., Swanson, R., and Preuss, D. (2004) Pollen and stigma structure and function: the role of diversity in pollination. Plant Cell 16: S84-S97.
Gu, Y., Vernoud, V., Fu, Y., and Yang, Z. (2003) ROP GTPases regulation of pollen tube growth through the dynamics of tip-localized F-actin. J. Exp. Bot. 54: 93-101.
Heslop-Harrison, J. (1979) An interpretation of the hydrodynamics of pollen. Am. J. Bot. 66:737-743.
Heslop-Harrison, Y., and Heslop-Harrison, J. (1992) Germination of monocoplate angiosperm pollen evolution of the actin cytoskeleton and wall during hydration activation and tube emergence. Ann. Bot. 69: 385-394.
Holmes-Davis, R., Tanaka, C. K., Vensel, W. H., Hurkman, W. J., and McCormick, S. (2005) Proteome mapping of mature pollen of Arabidopsis thaliana. Proteomics 5: 4864-4884.
Honys, D., and Twell, D. (2003) Comparative analysis of the Arabidopsis pollen transcriptome. Plant Physiol. 132: 640-652.
Hsu, S.-W., and Wang, C.-S. (2006) A lily pollen-specific cDNA encoding the Cdc42/Rac-interactive-binding motif-containing protein associated with pollen tube growth. Physiol. Plant. 126: 232-242.
Hsu, Y.-F., Wang, C.-S., and Raja, R. (2007) Gene expression pattern at desiccation in the anther of Lilium longiflorum. Planta 226: 311-322.
Huang, J.–C., Lin, S. -M., and Wang, C.-S. (2000) A pollen-specific and desiccation-associated transcripts in Lilium longiflorum during development and stress. Plant Cell Physiol. 41: 477-485.
Johnson, S. A., and McCormick, S. (2001) Pollen germinates precociously in the anthers raring-to-go, an Arabidopsis gametophytic mutant. Plant Physiol. 126: 685-695.
Johri, B. M. (1984) Embryology of angiosperms. Springer-Verlag, Berlin Heidelberg New York Tokyo, pp 53-76.
Kamalay, J. C., and Goldberg, R. B. (1980) Regulation of structural gene expression in tobacco. Cell 19: 935-946.
Ko, C.-W., Yang, C.-Y., and Wang, C.-S. (2002) A desiccation-induced transcript in lily (Lilium longiflorum) pollen. J. Plant Physiol. 159: 765-772.
Koltunow, A. M., Truettner, J., Cox, K. H., Wallroth, M., and Goldberg, R. B. (1990) Different temporal and spatial gene expression patterns occur during anther development. Plant Cell 2: 1201-1224.
Mascarenhas, J. P. (1990) Gene activity during pollen development. Annu. Rev. Plant Physiol. 41: 317-338.
McCormick, S. (1993) Male Gametophyte Development. Plant Cell 5: 1265-1275.
McCormick, S. (2004) Control of male gametophyte development. Plant Cell 16: S142-S153.
Mogami, N., Shiota, H., and Tanaka, I. (2002) The identification of a pollen-specific LEA-like protein in Lilium longiflorum. Plant Cell and Environ. 25: 653-663.
Pina, C., Pinto, F., Feijo, J. A., and Becker, J. D. (2005) Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation. Plant Physiol. 138: 744-756.
Rosen, W. G. (1961) Studies on pollen tube chemotropism. Am. J. Bot. 48: 889-895.
Scott, R. (1994) Anther development: a molecular perspective. In the molecular biology of flowering. Brian R. J. Eds. CAB International. UK. pp 141-193.
Scott, R. J., Spielman, M., and Dickinson, H. G. (2004) Stamen structure and function. Plant Cell 16: S46-S60.
Shivanna, K. R., and Johri, B. M. (1985) The angiosperm pollen. Structure and function. Wiley Eastern Limited. India. pp 21-52
Tiwari, S. C., and Polito, V. S. (1988) Organization of the cytoskeleton in pollen tubes of Pyrus communis: A study employing conventional and freeze-substitution electron microscopy, immunofluorescence and rhodamine-phalloidin. Protoplasma 147: 100-112.
Twell, D., Soon, K. P., and Eric, L. (1998) Asymmertric division and cell-fate determination in developing pollen. Trends Plant Sci. 3: 305-310.
Wagner, V. T., Cresti, M., Salvaticl, P., and Tiezzl, A. (1990) Changes in volume, surface area, and frequency of nuclear pores of the vegetative nucleus of tobacco pollen in fresh, hydrated, and activated conditions. Planta 181: 304-309.
Wang, C.-S., Liau, Y.-E. Huang, J.-C., Wu, T.-D., Su, C.-C., and Lin, C. H. (1998) Characterization of a desiccation-related protein in lily pollen during development and stress. Plant Cell Physiol. 39: 1307-1314.
Wang, C.-S., Lin, S. -M., and Wei, S.-L. (1999) A stress-inducible protein associated with desiccation in lily pollen. Bot. Bull. Acad. Sin. 40: 199-205
Wang, C.-S., Walling, L. L., Eckard, K. J., and Lord, E. M. (1992) Immunological characterization of a tapetal protein in developing anthers of Lilium longiflorum. Plant Physiol. 99: 822-829.
Wang, C.-S., Wu, T.-D., Chung, C.-K. W., and Lord, E. M. (1996) Two classes of pollen-specific, heat-stable proteins in Lilium longiflorum. Physiol. Plant. 97: 643-650.
Willing, R. P., Bashe, D., and Mascarenhas, J. P. (1984) An analysis of the complexity and diversity of mRNAs from pollen and shoots of Tradescantia. Plant Physiol. 75: 865-868.
Wolters-Arts, M., Lush, W. M., and Mariani, C. (1998) Lipids are required for directional pollen-tube growth. Nature 392: 818-821.
Wu, H. M., and Cheun, A. Y. (2000) Programmed cell death in plant reproduction. Plant Mol. Biol. 44: 267-281.
Yang, C.-Y., Chen, Y.-C., Jauh, G. Y. and Wang, C.-S. (2005) A lily ASR protein involves Abscisic Acid signaling and confers drought and salt resistance in Arabidopsis. Plant Physiol. 139: 836-846.
鄭兆伶。2006。鐵炮百合LLP-Rop1基因於花粉生長之功能性分析。國立中興大學碩士論文。
Ali, R., Zielinski, R.E., and Berkowitz, G.A. (2006) Expression of plant cyclic nucleotide-gated cation channels in yeast. J. Exp. Bot. 57: 125-138.
Allwood, E.G., Smertenko, A.P., and Hussey, P.J. (2001) Phosphorylation of plant actin-depolymerizing factor by calmodulinlike domain protein kinase. FEBS Lett. 499: 97–100
Assmann, S.M. (2002) Heterotrimeric and unconventional GTP binding proteins in plant cell signaling. Plant Cell 14: S355-S373.
Barwe, S.P., Sathiyabama, M., and Jayabaskaran, C. (2001) Induction of chitinase activity by exogenous cytokinins in excised dark-grown cucumber cotyledons: involvement of Ca2+ and staurosporine-sensitive protein kinase(s) in cytokinin signaling. J. Plant Physiol. 158: 1–7
Baxter-Burrell, A., Yang, Z., Springer, P.S., and Bailey-Serres, J. (2002) RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen deprivation tolerance. Science 296: 2026-2028.
Bedinger, P.A., and Edgerton, M.D. (1990) Developmental staging of maize microspores reveals a transition in developing microspore proteins. Plant Physiol. 92: 474-479.
Berken, A. (2006) ROPs in the spotlight of plant signal transduction. Cell Mol. Life Sci. 63: 2446-2459.
Bischoff, F., Molendijk, A., Rajendrakumar, C. S., and Palme, K. (1999) GTP-binding proteins in plants. Cell. Mol. Life Sci. 55: 233-256.
Bischoff, F., Vahlkamp, L., Molendijk, A., and Palme, K. (2000) Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis. Plant Mol. Biol. 42: 515-530.
Boavida, L.C., and McCormick, S. (2007) Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana. Plant J. 52: 570-582
Brembu, T., Winge, P., and Bones, A.M. (2005) The small GTPase AtRAC2/ROP7 is specifically expressed during late stages of xylem differentiation in Arabidopsis. J. Exp. Bot. 56: 2465-2476.
Cardenas, L., Lovy-Wheeler, A., Kunkel, J.G., and Hepler, P.K. (2008) Pollen tube growth oscillations and intracellular calcium levels are reversibly modulated by actin polymerization. Plant Physiol. 146: 1611-1621.
Chen, C.Y., Cheung, A.Y., and Wu, H.M. (2003) Actin-depolymerizing factor mediates Rac/Rop GTPase-regulated pollen tube growth. Plant Cell 15: 237-249
Chen, C.Y., Wong, E.I., Vidali, L., Estavillo, A., Hepler, P.K., Wu, H.M., and Cheung, A.Y. (2002) The regulation of actin organization by actin-depolymerizing factor in elongating pollen tubes. Plant Cell 14: 2175-2190.
Chen, T., Wu, X., Chen, Y., Li, X., Huang, M., Zheng, M., Baluska, F., Samaj, J., and Lin, J. (2009) Combined proteomic and cytological analysis of Ca2+-calmodulin regulation in Picea meyeri pollen tube growth. Plant Physiol. 149: 1111-1126.
Cheung, A.Y., and Wu, H.M. (2008) Structural and signaling networks for the polar cell growth machinery in pollen tubes. Annu. Rev. Plant Biol. 59: 547-572.
Christensen, T.M., Vejlupkova, Z., Sharma, Y.K., Arthur, K.M., Spatafora, J.W., Albright, C.A., Meeley, R.B., Duvick, J.P., Quatrano, R.S., and Fowler, J.E. (2003) Conserved subgroups and developmental regulation in the monocot rop gene family. Plant Physiol.133: 1791-1808.
Demidchik, V., and Maathuis, F.J. (2007) Physiological roles of nonselective cation channels in plants: from salt stress to signalling and development. New Phytol. 175: 387-404.
Detchepare, S., Heizmann, P., and Dumas, C. (1989) Changes in protein patterns and protein synthesis during anther development in Brassica oleracea. J. Plant Physiol. 135: 129-137.
Dickinson, D.B. (1978) Influence of borate and pentaerythritol concentrations on germination and tube growth of Lilium longiflorum pollen. J. Am. Soc. Horti. Sci. 103: 413-416.
Dodd, A.N., Kudla, J., and Sanders, D. (2010) The language of calcium signaling. Annu. Rev. Plant Biol. 61: 593-620.
Faure, J., and Dagher, M.C. (2001) Interactions between Rho GTPases and Rho GDP dissociation inhibitor (Rho-GDI). Biochimie 83: 409-414.
Filichkin, S.A., Leonard, J.M., Monteros, A., Liu, P.P., and Nonogaki, H. (2004) A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development. Plant Physiol. 134: 1080-1087.
Frietsch, S., Wang, Y.F., Sladek, C., Poulsen, L.R., Romanowsky, S.M., Schroeder, J.I., and Harper, J.F. (2007) A cyclic nucleotide-gated channel is essential for polarized tip growth of pollen. Proc. Natl. Acad. Sci .USA 104: 14531-14536
Golovkin, M., and Reddy, A.S. (2003) A calmodulin-binding protein from Arabidopsis has an essential role in pollen germination. Proc. Natl. Acad. Sci. USA 100: 10558-10563
Gu, Y., Fu, Y., Dowd, P., Li, S., Vernoud, V., Gilroy, S., and Yang, Z. (2005) A Rho family GTPase controls actin dynamics and tip growth via two counteracting downstream pathways in pollen tubes. J. Cell Biol. 169: 127-138.
Gu, Y., Wang, Z., and Yang, Z. (2004) ROP/RAC GTPase: an old new master regulator for plant signaling. Curr. Opin. Plant Biol. 7: 527-736.
Guo, K.M., Babourina, O., Christopher, D.A., Borsics, T., and Rengel, Z. (2008) The cyclic nucleotide-gated channel, AtCNGC10, influences salt tolerance in Arabidopsis. Physiol. Plant. 134: 499-507.
Hamilton, D.W., Hills, A., Kohler, B., and Blatt, M.R. (2000) Ca2+ channels at the plasma membrane of stomatal guard cells are activated by hyperpolarization and abscisic acid. Proc. Natl. Acad. Sci. U S A 97: 4967-4972.
Hancock, J.F., Cadwallader, K., Paterson, H., and Marshall, C.J. (1991) A CAAX or a CAAL motif and a second signal are sufficient for plasma membrane targeting of ras proteins. EMBO J. 10: 4033-4039.
Harada, A., and Shimazaki, K. (2009) Measurement of changes in cytosolic Ca2+ in Arabidopsis guard cells and mesophyll cells in response to blue light. Plant Cell Physiol. 50: 360-373.
Hepler, P.K. (2005) Calcium: a central regulator of plant growth and development. Plant Cell 17: 2142-2155
Holdaway-Clarke, T.L., and Hepler, P.K. (2003) Control of pollen tube growth: role of ion gradient and fluxes. New Phytol. 159: 539-563
Hrabak, E.M., Chan, C.W., Gribskov, M., Harper, J.F., Choi, J.H., Halford, N., Kudla, J., Luan, S., Nimmo, H.G., Sussman, M.R., Thomas, M., Walker-Simmons, K., Zhu, J.K., and Harmon, A.C., (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol. 132: 666-680.
Hsu, S.-W., and Wang, C.-S. (2006) A lily pollen-specific cDNA encoding the Cdc42/Rac-interactive-binding motif-containing protein associated with pollen tube growth. Physiol. Plant. 126: 232-242.
Hsu, Y.-F., Wang, C.-S., and Raja, R. (2007) Gene expression pattern at desiccation in the anther of Lilium longiflorum. Planta 226: 311-322.
Huang, J.-C., Lin, S.-M., and Wang, C.-S. (2000) Characterization of a pollen-specific and desiccation-associated transcript in Lilium longiflorum during development and stress. Plant Cell Physiol. 41: 477-485.
Hwang, J.U., Gu, Y., Lee, Y.J., and Yang, Z. (2005) Oscillatory ROP GTPase activation leads the oscillatory polarized growth of pollen tubes. Mol. Biol. Cell 16: 5385-5399
Iwano, M., Entani, T., Shiba, H., Kakita, M., Nagai, T., Mizuno, H., Miyawaki, A., Shoji, T., Kubo, K., Isogai, A., and Takayama, S. (2009) Fine-tuning of the cytoplasmic Ca2+ concentration is essential for pollen tube growth. Plant Physiol. 150: 1322-1334
Jung, Y.H., Agrawal, G.K., Rakwal, R., Kim, J.A., Lee, M.O., Choi, P.G., Kim, Y.J., Kim, M.J., Shibato, J., Kim, S.H., Iwahashi, H., and Jwa, N.S. (2006) Functional characterization of OsRacB GTPase-a potentially negative regulator of basal disease resistance in rice. Plant Physiol. Biochem. 44: 68-77.
Kong, L., Wang, M., Wang, Q., Wang, X., and Lin, J. (2006) Protein phosphatases 1 and 2A and the regulation of calcium uptake and pollen tube development in Picea wilsonii. Tree Physiol. 26: 1001-1012
Kost, B. (2008) Spatial control of Rho (Rac-Rop) signaling in tip-growing plant cells. Trends Cell Biol. 18: 119-127.
Kost, B., Lemichez, E., Spielhofer, P., Hong, Y., Tolias, K., Carpenter, C., and Chua, N.H. (1999) Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth. J. Cell Biol. 145: 317-330.
Kudla, J., Batistic, O., and Hashimoto, K. (2010) Calcium signals: the lead currency of plant information processing. Plant Cell 22: 541-563.
Laohavisit, A., Mortimer, J.C., Demidchik, V., Coxon, K.M., Stancombe, M.A., Macpherson, N., Brownlee, C., Hofmann, A., Webb, A.A.R., Miedema, H., Battey, N.H., and Davies, J.M. (2009) Zea mays annexins modulate cytosolic free Ca2+ and generate a Ca2+-permeable conductance. Plant Cell 21: 479-493.
Lee, Y.J., and Yang, Z. (2008) Tip growth: signaling in the apical dome. Curr. Opin. Plant Biol. 11: 662-671.
Li, H., Lin, Y., Heath, R.M., Zhu, M.X., Yang, Z. (1999) Control of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to tip-localized calcium influx. Plant Cell 11: 1731-1742
Li, J., Zhu, S., Song, X., Shen, Y., Chen, H., Yu, J., Yi, K., Liu, Y., Karplus, V.J., Wu, P., and Deng, X.W. (2006) A rice glutamate receptor-like gene is critical for the division and survival of individual cells in the root apical meristem. Plant Cell 18: 340-349.
Li, S., Gu, Y., Yan, A., Lord, E., and Yang, Z.B. (2008) RIP1 (ROP Interactive Partner 1)/ICR1 Marks Pollen Germination Sites and May Act in the ROP1 Pathway in the Control of Polarized Pollen Growth. Mol. Plant 1: 1021-1035.
Luan, S. (2009) The CBL-CIPK network in plant calcium signaling. Trends. Plant Sci.14: 37-42.
Ludwig, A.A., Romeis, T., and Jones, J.D. (2004) CDPK-mediated signalling pathways: specificity and cross-talk. J Exp. Bot. 55: 181-188.
Luo, M., Gu, S.H., Zhao, S.H., Zhang, F., and Wu, N.H. (2006) Rice GTPase OsRacB: potential accessory factor in plant salt-stress signaling. Acta Biochim. Biophys. Sin. (Shanghai) 38: 393-402.
Maliga, P., Klessig, D.F., Cashmore, A.R., Gruissem, W., and Varner, J.E. (1995) Methods in plant molecular biology. A laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
McCormick, S. (1993) Male gametophyte development. Plant Cell 5: 1265-1275.
Meyerhoff, O., Muller, K., Roelfsema, M.R., Latz, A., Lacombe, B., Hedrich, R., Dietrich, P., and Becker, D. (2005) AtGLR3.4, a glutamate receptor channel-like gene is sensitive to touch and cold. Planta 222: 418-427.
Miedema, H., Demidchik, V., Very, A.A., Bothwell, J.H., Brownlee, C., and Davies, J.M. (2008) Two voltage-dependent calcium channels co-exist in the apical plasma membrane of Arabidopsis thaliana root hairs. New Phytol. 179: 378-385.
Miki-Hirosige, H., Yamanaka, Y., Nakamura, S., Kurata, S., and Hirano, H. (2004) Changes of protein profiles during pollen development in L. longiXorum. Sex. Plant Reprod. 16: 209-214.
Mortimer, J.C., Laohavisit, A., Macpherson, N., Webb, A., Brownlee, C., Battey, N.H., and Davies, J.M. (2008) Annexins: multifunctional components of growth and adaptation. J Exp. Bot. 59: 533-544.
Myers, C., Romanowsky, S.M., Barron, Y.D., Garg, S., Azuse, C.L., Curran, A., Davis, R.M., Hatton, J., Harmon, A.C., and Harper, J.F. (2009) Calcium-dependent protein kinases regulate polarized tip growth in pollen tubes. Plant J. 59: 528-539
Nibau, C., Wu, H.M., and Cheung, A.Y. (2006) RAC/ROP GTPases ''hubs'' for signal integration and diversification in plants. Trends Plant Sci. 11: 309-315.
Park, J., Gu, Y., Lee, Y., Yang, Z., and Lee, Y. (2004) Phosphatidic acid induces leaf cell death in Arabidopsis by activating the Rho-related small G protein GTPase-mediated pathway of reactive oxygen species generation. Plant Physiol. 134: 129-136.
Pei, Z.M., Murata, Y., Benning, G., Thomine, S., Klusener, B., Allen, G.J., Grill, E., and Schroeder, J.I. (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406: 731-734.
Putnam-Evans, C., Harmon, A.C., Palevitz, B.A., Fechheimer, M., and Cormier, M.J. (1989) Calcium-dependent protein kinase is localized with F-actin in plant cells. Cell Motil. Cytoskel. 12: 12–22
Qi, Z., Stephens, N.R., and Spalding, E.P. (2006) Calcium entry mediated by GLR3.3, an Arabidopsis glutamate receptor with a broad agonist profile. Plant Physiol. 142: 963-971.
Qu, H.Y., Shang, Z.L., Zhang, S.L., Liu, L.M., and Wu, J.Y. (2007) Identification of hyperpolarization-activated calcium channels in apical pollen tubes of Pyrus pyrifolia. New Phytol. 174: 524-536
Ridley, A. (2000) Rho in GTPases, A. Hall, ed (Oxford: Oxford University Press) pp 89-136.
Schmidt, A., and Hall, A. (2002) Guanine nucleotide exchange factors for Rho GTPases: turning on the switch. Genes Dev. 16: 1587-1609.
Scholz-Starke, J., Buttner, M., and Sauer, N. (2003) AtSTP6, a new pollen-specific H+-monosaccharide symporter from Arabidopsis. Plant Physiol. 131: 70-77.
Shang, Z.L., Ma, L.G., Zhang, H.L., He, R.R., Wang, X.C., Cui, S.J., and Sun, D.Y. (2005) Ca2+ influx into lily pollen grains through a hyperpolarization-activated Ca2+-permeable channel which can be regulated by extracellular CaM. Plant Cell Physiol. 46: 598-608
Shaw, S.L. and Long, S.R. (2003) Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells. Plant Physiol. 131: 976-984.
Sivitz, A.B., Reinders, A., and Ward, J.M. (2008) Arabidopsis sucrose transporter AtSUC1 is important for pollen germination and sucrose-induced anthocyanin accumulation. Plant Physiol. 147: 92-100
Stephens, N.R., Qi, Z., and Spalding, E.P. (2008) Glutamate receptor subtypes evidenced by differences in desensitization and dependence on the GLR3.3 and GLR3.4 genes. Plant Physiol. 146: 529-538.
Szucs, A., Dorjgotov, D., Otvos, K., Fodor, C., Domoki, M., Gyorgyey, J., Kalo, P., Kiss, G.B., Dudits, D., and Feher, A. (2006) Characterization of three Rop GTPase genes of alfalfa (Medicago sativa L.). Biochim. Biophys. Acta 1759: 108-115.
Takai, Y., Sasaki, T., and Matozaki, T. (2001) Small GTP-Binding proteins. Physiol. Rev. 81: 153-208.
Tao, L.Z., Cheung, A.Y., Nibau, C., and Wu H.M. (2005) RAC GTPases in tobacco and Arabidopsis mediate auxin-induced formation of proteolytically active nuclear protein bodies that contain AUX/IAA proteins. Plant Cell 17: 2369-2383.
Thion, L., Mazars, C., Nacry, P., Bouchez, D., Moreau, M., Ranjeva, R., and Thuleau, P. (1998) Plasma membrane depolarization-activated calcium channels, stimulated by microtubule-depolymerizing drugs in wild-type Arabidopsis thaliana protoplasts, display constitutively large activities and a longer half-life in ton 2 mutant cells affected in the organization of cortical microtubules. Plant J. 13: 603-610.
Trotochaud, A.E., Hao, T., Wu, G., Yang, Z., and Clark, S.E. (1999) The CLAVATA1 receptor-like kinase requires CLAVATA3 for its assembly into a signaling complex that includes KAPP and a Rho-related protein. Plant Cell 11: 393-406.
Urquhart, W., Gunawardena, A.H., Moeder, W., Ali, R., Berkowitz, G.A., and Yoshioka, K. (2007) The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca2+ dependent manner. Plant Mol. Biol. 65: 747-761.
Vernoud, V., Horton, A. C., Yang, Z., and Nielsen, E. (2003) Analysis of the small GTPases gene superfamily of Arabidopsis thaliana. Plant Physiol. 131: 1191-1208.
Verwoerd, T.C., Dekker, B.M., and Hoekema, A. (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 17: 2362.
Walch-Liu, P., Liu, L.H., Remans, T., Tester, M., and Forde, B.G. (2006) Evidence that L-glutamate can act as an exogenous signal to modulate root growth and branching in Arabidopsis thaliana. Plant Cell Physiol. 47: 1045-1057.
Wang, C.-S., Wu, T.-D., Chung, C.-K.W., and Lord, E.M. (1996). Two classes of pollen-specific, heat-stable proteins in Lilium longiflorum. Physiol. Plant. 97: 643-650.
Wang, Y., Zhang, W.Z., Song, L.F., Zou, J.J., Su, Z., and Wu, W.H. (2008) Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis. Plant Physiol. 148: 1201-1211
Wang, Y.F., Fan, L.M., Zhang, W.Z., Zhang, W., and Wu, W.H. (2004) Ca2+-permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilaments. Plant Physiol. 136: 3892-3904
White, P.J., Bowen, H.C., Demidchik, V., Nichols, C., and Davies, J.M. (2002). Genes for calcium-permeable channels in the plasma membrane of plant root cells. Biochim. Biophys. Acta 1564: 299–309.
Willing, R.P., Bashe, D., and Mascarenhas, J.P. (1988) An analysis of the quantity and diversity of messenger RNAs from pollen and shoot of Zea mays. Theor. Appl. Genet. 75: 751–753.
Winge, P., Brembu, T., Kristensen, R., and Bones, A. M. (2000) Genetic structure and evolution of RAC-GTPases in Arabidopsis thaliana. Genetics 156: 1959-1971.
Wu, G., Gu, Y., Li, S., and Yang, Z. (2001) A genome-wide analysis of Arabidopsis Rop-interactive CRIB motif-containing proteins that act as Rop GTPase targets. Plant Cell 13: 2841-2856.
Wu, Y., Xu, X., Li, S., Liu, T., Ma, L., and Shang, Z. (2007) Heterotrimeric G-protein participation in Arabidopsis pollen germination through modulation of a plasmamembrane hyperpolarization-activated Ca2+-permeable channel. New Phytol. 176: 550-559.
Xin, Z., Zhao, Y., and Zheng, Z.L. (2005) Transcriptome analysis reveals specific modulation of abscisic acid signaling by ROP10 small GTPase in Arabidopsis. Plant Physiol. 139: 1350-1365.
Xiong, L., Ishitani, M., Lee, H., and Zhu, J.-K. (2001) The Arabidopsis LOS/ABA3 locus encodes a molybdenum cofactor sulfurase and modulates cold stress- and osmotic stress-responsive gene expression. Plant Cell 13: 2063-2083.
Xiong, L., and Zhu, J.K. (2003) Regulation of abscisic acid biosynthesis. Plant Physiol. 133: 29-36.
Xu, J., Li, H., Chen, L., Wang, Y., Liu, L., He, L., and Wu, W.H. (2006). A protein kinase, interacting with two calcineurin B-like proteins, regulates K+ TRANSPORTER AKT1 in Arabidopsis. Cell 125: 1347–1360
Yang, Z. (2002) Small GTPases: Versatile signaling switches in plants. Plant Cell 12: S375-S388.
Yang, Z., and Fu, Y. (2007) ROP/RAC GTPase signaling. Curr. Opin. Plant Biol. 10: 490-494.
Yoon, G.M., Dowd P.E., Gilroy, S., and McCubbin, A.G. (2006) Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity. Plant Cell 18: 867-878
Young, J.J., Mehta, S., Israelsson, M., Godoski, J., Grill, E., and Schroeder, J.I. (2006) CO2 signaling in guard cells: calcium sensitivity response modulation, a Ca2+-independent phase, and CO2 insensitivity of the gca2 mutant. Proc. Natl. Acad. Sci. U S A 103: 7506-7511.
Zhang, D., Wengier, D., Shuai, B., Gui, C.P., Muschietti, J., McCormick, S., and Tang, W.H. (2008) The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth. Plant Physiol. 148: 1368-1379.
Zhang, Y., and McCormick, S. (2008) Regulation of pollen tube polarity: Feedback loops rule. Plant Signal Behav. 3: 345-347
Zheng, Z.L., Nafisi, M., Tam, A., Li, H., Crowell, D.N., Chary, S.N., Schroeder, J.I., Shen, J., and Yang, Z. (2002) Plasma membrane-associated ROP10 small GTPase is a specific negative regulator of abscisic acid responses in Arabidopsis. Plant Cell 14: 2787-2797.
Zheng, Z.-L., and Yang, Z. (2000) The Rop GTPases switch turns on polar growth in pollen. Trends Plant Sci. 5: 298-303.
Zhou, L., Fu, Y., and Yang, Z. (2009) A genome-wide functional characterization of Arabidopsis regulatory calcium sensors in pollen tubes. J. Integr. Plant Biol. 51: 751–761
Aya, K., Ueguchi-Tanaka, M., Kondo, M., Hamada, K., Yano, K., Nishimura, M., and Matsuoka, M. (2009) Gibberellin modulates anther development in rice via the transcriptional regulation of GAMYB. Plant Cell 21: 1453-1472.
Bantignies, B., Seguin, J., Muzac, I., Dedaldechamp, F., Gulick, P., and Ibrahim, R. (2000) Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots. Plant Mo.l Biol. 42: 871-881.
Bate, N., and Twell, D. (1998) Functional architecture of a late pollen promoter: pollen-specific transcription is developmentally regulated by multiple stage-specific and co-dependent activator elements. Plant Mol. Biol. 37: 859-869.
Biesiadka, J., Bujacz, G., Sikorski, M.M., and Jaskolski, M. (2002) Crystal structures of two homologous pathogenesis-related proteins from yellow lupine. J. Mol. Biol. 319: 1223-1234.
Brandazza, A., Angeli, S., Tegoni, M., Cambillau, C., and Pelosi, P. (2004) Plant stress proteins of the thaumatin-like family discovered in animals. FEBS Lett. 572: 3-7.
Breda, C., Sallaud, C., el-Turk, J., Buffard, D., de Kozak, I., Esnault, R., and Kondorosi, A. (1996) Defense reaction in Medicago sativa: a gene encoding a class 10 PR protein is expressed in vascular bundles. Mol. Plant Microbe. Interact. 9: 713-719.
Breiteneder, H., Ferreira, F., Reikerstorfer, A., Duchene, M., Valenta, R., Hoffmann-Sommergruber, K., Ebner, C., Breitenbach, M., Kraft, D., and Scheiner, O. (1992) Complementary DNA cloning and expression in Escherichia coli of Aln g I, the major allergen in pollen of alder (Alnus glutinosa). J. Allergy Clin. Immunol. 90: 909-917.
Breiteneder, H., Hoffmann-Sommergruber, K., O''Riordain, G., Susani, M., Ahorn, H., Ebner, C., Kraft, D., and Scheiner, O. (1995) Molecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDa tree pollen allergens. Eur. J. Biochem. 233: 484-489.
Bufe, A., Spangfort, M.D., Kahlert, H., Schlaak, M., and Becker, W.M. (1996) The major birch pollen allergen, Bet v 1, shows ribonuclease activity. Planta 199: 413-415.
Cercos, M., Gomez-Cadenas, A., and Ho, T.H. (1999) Hormonal regulation of a cysteine proteinase gene, EPB-1, in barley aleurone layers: cis- and trans-acting elements involved in the co-ordinated gene expression regulated by gibberellins and abscisic acid. Plant J. 19: 107-118.
Chen, Z.Y., Brown, R.L., Rajasekaran, K., Damann, K.E., and Cleveland, T.E. (2006) Identification of a Maize Kernel Pathogenesis-Related Protein and Evidence for Its Involvement in Resistance to Aspergillus flavus Infection and Aflatoxin Production. Phytopath.96: 87-95.
Coutos-Thevenot, P., Poinssot, B., Bonomelli, A., Yean, H., Breda, C., Buffard, D., Esnault, R., Hain, R., and Boulay, M. (2001) In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen-inducible PR 10 promoter. J. Exp. Bot. 52: 901-910.
Crowell, D.N., John, M.E., Russell, D., and Amasino, R.M. (1992) Characterization of a stress-induced, developmentally regulated gene family from soybean. Plant Mol. Biol. 18: 459-466.
De Grauwe, L., Vriezen, W.H., Bertrand, S., Phillips, A., Vidal, A.M., Hedden, P., and Van Der Straeten, D. (2007) Reciprocal influence of ethylene and gibberellins on response-gene expression in Arabidopsis thaliana. Planta 226: 485-498.
Despres, C., Subramaniam, R., Matton, D.P., and Brisson, N. (1995) The Activation of the potato PR-10a gene requires the phosphorylation of the nuclear factor PBF-1. Plant Cell 7: 589-598.
Dubos, C., and Plomion, C. (2001) Drought differentially affects expression of a PR-10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlings. J. Exp. Bot. 52: 1143-1144.
Ekramoddoullah, A.K.M., Yu, X., Sturrock, R., Zamani, A., and Taylor, D. (2000) Detection and seaonal expression pattern of a pathogenesis-related protein (PR-10) in Douglas-fir (Pseudotsuga menziesii) tissues. Physiol. Plant. 110: 240-247.
Fernandes, H., Bujacz, A., Bujacz, G., Jelen, F., Jasinski, M., Kachlicki, P., Otlewski, J., Sikorski, M.M., and Jaskolski, M. (2009) Cytokinin-induced structural adaptability of a Lupinus luteus PR-10 protein. FEBS J. 276: 1596-1609.
Flores, T., Alape-Giron, A., Flores-Diaz, M., and Flores, H.E. (2002) Ocatin. A novel tuber storage protein from the andean tuber crop oca with antibacterial and antifungal activities. Plant Physiol. 128: 1291-1302.
Fristensky, B., Horovitz, D., and Hadwiger, L.A. (1988) cDNA sequences for pea disease resistance response genes. Plant Mol. Biol. 11: 713-715.
Glazebrook, J. (2005) Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu. Rev. Phytopathol. 43: 205-227.
Hashimoto, M., Kisseleva, L., Sawa, S., Furukawa, T., Komatsu, S., and Koshiba, T. (2004) A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant Cell Physiol. 45: 550-559.
Hirano, K., Aya, K., Hobo, T., Sakakibara, H., Kojima, M., Shim, R.A., Hasegawa, Y., Ueguchi-Tanaka, M., and Matsuoka, M. (2008) Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice. Plant Cell Physiol. 49: 1429-1450.
Hu, X., Bidney, D.L., Yalpani, N., Duvick, J.P., Crasta, O., Folkerts, O., and Lu, G. (2003) Overexpression of a gene encoding hydrogen peroxide-generating oxalate oxidase evokes defense responses in sunflower. Plant Physiol. 133: 170-181.
Huang, J.C., Chang, F.C., and Wang, C.S. (1997) Characterization of a lily tapetal transcript that shares sequence similarity with a class of intracellular pathogenesis-related (IPR) proteins. Plant Mol. Biol. 34: 681-686.
Izhaki, A., Borochov, A., Zamski, E., and Weiss, D. (2002) Gibberellin regulates post-microsporogenesis processes in petunia anthers. Physiol. Plant 115: 442-447.
Itzhaki, H., Maxson, J.M., and Woodson, W.R. (1994) An ethylene-responsive enhancer element is involved in the senescence-related expression of the carnation glutathione-S-transferase (GST1) gene. Proc. Natl. Acad. Sci. U S A 91: 8925-8929.
Jwa, N.S., Kumar Agrawal, G., Rakwal, R., Park, C.H., and Prasad Agrawal, V. (2001) Molecular cloning and characterization of a novel Jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves. Biochem. Biophys. Res. Commun. 286: 973-983.
Kaneko, M., Itoh, H., Inukai, Y., Sakamoto, T., Ueguchi-Tanaka, M., Ashikari, M., and Matsuoka, M. (2003) Where do gibberellin biosynthesis and gibberellin signaling occur in rice plants? Plant J. 35: 104-115.
Kao, F.I., Cheng, Y.Y., Chow, T.Y., Chen, H.H., Liu, S.M., Cheng, C.H., and Chung, M.C. (2006) An integrated map of Oryza sativa L. chromosome 5. Theor. Appl. Genet. 112: 891-902.
Kim, S.T., Kang, S.Y., Wang, Y., Kim, S.G., Hwang du, H., and Kang, K.Y. (2008) Analysis of embryonic proteome modulation by GA and ABA from germinating rice seeds. Proteomics 8: 3577-3587.
Kim, S.Y., Chung, H.J., and Thomas, T.L. (1997) Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo-specification elements in the Dc3 promoter using a modified yeast one-hybrid system. Plant J. 11: 1237-1251.
Lee, M.W., Qi, M., and Yang, Y. (2001) A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death. Mol. Plant Microbe. Interact. 14: 527-535.
Liu, J.-J., and Ekramoddoullah, A.K.M. (2004) Characterization, expression and evolution of two novel subfamilies of Pinus monticola cDNAs encoding pathogenesis-related (PR)-10 proteins. Tree Physiol. 24: 1377-1385.
Liu, J.-J., and Ekramoddoullah, A.K.M. (2006) The family 10 of plant pathogenesis-related proteins: their structure, regulation, and function in response to biotic and abiotic stresses. Physiol. Mol. Plant Pathol. 68: 3-13.
Liu, J.-J., Ekramoddoullah, A.K.M., Piggott, N., and Zamani, A. (2005) Molecular cloning of a pathogen/wound-inducible PR10 promoter from Pinus monticola and characterization in transgenic Arabidopsis plants. Planta 221: 159-169.
Liu, J.-J., Ekramoddoullah, A.K.M., and Yu, X. (2003) Differential expression of multiple PR10 proteins in western white pine following wounding, fungal infection and cold-hardening. Physiol. Plant. 119: 544-553.
Liu, Y.G., and Whittier, R.F. (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25: 674-681.
Lo, S.C., Hipskind, J.D., and Nicholson, R.L. (1999) cDNA cloning of a sorghum pathogenesis-related protein (PR-10) and differential expression of defense-related genes following inoculation with Cochliobolus heterostrophus or Colletotrichum sublineolum. Mol. Plant Microbe. Interact. 12: 479-489.
Markovic-Housley, Z., Degano, M., Lamba, D., von Roepenack-Lahaye, E., Clemens, S., Susani, M., Ferreira, F., Scheiner, O., and Breiteneder, H. (2003) Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier. J. Mol. Biol. 325: 123-133.
Mattheus, N., Ekramoddoullah, A.K., and Lee, S.P. (2003) Isolation of high-quality RNA from white spruce tissue using a three-stage purification method and subsequent cloning of a transcript from the PR-10 gene family. Phytochem. Anal. 14: 209-215.
Matton, D.P., and Brisson, N. (1989) Cloning, expression, and sequence conservation of pathogenesis-related gene transcripts of potato. Mol. Plant Microbe. Interact. 2: 325-331.
Matton, D.P., Prescott, G., Bertrand, C., Camirand, A., and Brisson, N. (1993) Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato. Plant Mol. Biol. 22: 279-291.
McGee, J.D., Hamer, J.E., and Hodges, T.K. (2001) Characterization of a PR-10 pathogenesis-related gene family induced in rice during infection with Magnaporthe grisea. Mol. Plant Microbe. Interact. 14: 877-886.
Midoh, N., and Iwata, M. (1996) Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice. Plant Cell Physiol. 37: 9-18.
Morita, A., Umemura, T., Kuroyanagi, M., Futsuhara, Y., Perata, P., and Yamaguchi, J. (1998) Functional dissection of a sugar-repressed alpha-amylase gene (RAmy1 A) promoter in rice embryos. FEBS Lett. 423: 81-85.
Mylona, P., Moerman, M., Yang, W.C., Gloudemans, T., Van de Kerckhove, J., van Kammen, A., Bisseling, T., and Franssen, H.J. (1994) The root epidermis-specific pea gene RH2 is homologous to a pathogenesis-related gene. Plant Mol. Biol. 26: 39-50.
Nambara, E., Akazawa, T., and McCourt, P. (1991) Effects of the Gibberellin Biosynthetic Inhibitor Uniconazol on Mutants of Arabidopsis. Plant Physiol. 97: 736-738.
Park, C.J., Kim, K.J., Shin, R., Park, J.M., Shin, Y.C., and Paek, K.H. (2004) Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway. Plant J. 37: 186-198.
Passardi, F., Penel, C., and Dunand, C. (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci. 9: 534-540.
Puhringer, H., Moll, D., Hoffmann-Sommergruber, K., Watillon, B., Katinger, H., and Laimer da Camara Machado, M. (2000) The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogen-inducible. Plant Sci. 152: 35-50.
Rakwal, R., Agrawal, G.K., and Yonekura, M. (2001) Light-dependent induction of OsPR10 in rice (Oryza sati a L.) seedlings by the global stress signaling molecule jasmonic acid and protein phosphatase 2A inhibitors. Plant Sci. 161: 469-479.
Razem, F.A., Baron, K., and Hill, R.D. (2006) Turning on gibberellin and abscisic acid signaling. Curr. Opin. Plant Biol. 9: 454-459.
Rouster, J., Leah, R., Mundy, J., and Cameron-Mills, V. (1997) Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain. Plant J. 11: 513-523.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edition.. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Sels, J., Mathys, J., De Coninck, B.M., Cammue, B.P., and De Bolle, M.F. (2008) Plant pathogenesis-related (PR) proteins: a focus on PR peptides. Plant Physiol. Biochem. 46: 941-950.
Shah, J., and Klessig, D.F. (1996) Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related beta-1,3-glucanase gene, PR-2d. Plant J. 10: 1089-1101.
Smith, D.E., and Fisher, P.A. (1984) Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots. J. Cell Biol. 99: 20-28.
Somssich, I.E., Schmelzer, E., Bollmann, J., and Hahlbrock, K. (1986) Rapid activation by fungal elicitor of genes encoding "pathogenesis-related" proteins in cultured parsley cells. Proc. Natl. Acad. Sci. U S A 83: 2427-2430.
Srivastava, S., Fristensky, B., and Kav, N.N. (2004) Constitutive expression of a PR10 protein enhances the germination of Brassica napus under saline conditions. Plant Cell Physiol. 45: 1320-1324.
Srivastava, S., Rahman, M.H., Shah, S., and Kav, N.N. (2006) Constitutive expression of the pea ABA-responsive 17 (ABR17) cDNA confers multiple stress tolerance in Arabidopsis thaliana. Plant Biotechnol. J. 4: 529-549.
Steffens, B., Wang, J., and Sauter, M. (2006) Interactions between ethylene, gibberellin and abscisic acid regulate emergence and growth rate of adventitious roots in deepwater rice. Planta 223: 604-612.
Swoboda, I., Jilek, A., Ferreira, F., Engel, E., Hoffmann-Sommergruber, K., Scheiner, O., Kraft, D., Breiteneder, H., Pittenauer, E., and Schmid, E. (1995) Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning. J. Biol. Chem. 270: 2607-2613.
Takada, K., Ishimaru, K., Kamada, H., and Ezura, H. (2006) Anther-specific expression of mutated melon ethylene receptor gene Cm-ERS1/H70A affected tapetum degeneration and pollen grain production in transgenic tobacco plants. Plant Cell Rep. 25: 936-941.
Thevissen, K., Ghazi, A., De Samblanx, G.W., Brownlee, C., Osborn, R.W., and Broekaert, W.F. (1996) Fungal membrane responses induced by plant defensins and thionins. J. Biol. Chem. 271: 15018-15025.
Tonhosolo, R., D''Alexandri, F.L., de Rosso, V.V., Gazarini, M.L., and Matsumura, M.Y. (2009) Carotenoid biosynthesis in intraerythrocytic stages of Plasmodium falciparum. J. Biol. Chem. 284: 9974-9985.
Turck, F., Zhou, A., and Somssich, I.E. (2004) Stimulus-dependent, promoter-specific binding of transcription factor WRKY1 to Its native promoter and the defense-related gene PcPR1-1 in Parsley. Plant Cell 16: 2573-2585.
Tzeng, J.D., Hsu, S.W., Chung, M.C., Yeh, F.L., Yang, C.Y., Liu, M.C., Hsu, Y.F., and Wang, C.S. (2009) Expression and regulation of two novel anther-specific genes in Lilium longiflorum. J. Plant Physiol. 166: 417-427.
Van den Heuvel, K.J., Van Lipzig, R.H., Barendse, G.W., and Wullems, G.J. (2002) Regulation of expression of two novel flower-specific genes from tomato (Solanum lycopersicum) by gibberellin. J. Exp. Bot. 53: 51-59.
Van Loon, L.C., Pierpoint, W.S., Boller, T., and Conejero, V. (1994) Recommendation for naming plant pathogenesis-related proteins. Plant Mol. Biol. Rep. 12: 245-264.
van Loon, L.C., Rep, M. and Pieterse, C.M. (2006) Significance of inducible defense-related proteins in infected plants. Annu. Rev. Phytopathol. 44: 135-162.
Vanek-Krebitz, M., Hoffmann-Sommergruber, K., Laimer da Camara Machado, M., Susani, M., Ebner, C., Kraft, D., Scheiner, O., and Breiteneder, H. (1995) Cloning and sequencing of Mal d 1, the major allergen from apple (Malus domestica), and its immunological relationship to Bet v 1, the major birch pollen allergen. Biochem. Biophys. Res. Commun. 214: 538-551.
Verwoerd, T.C., Dekker, B.M., and Hoekema, A. (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Re.s 17: 2362.
Walter, M.H., Liu, J.W., Grand, C., Lamb, C.J., and Hess, D. (1990) Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens. Mol. Gen. Genet. 222: 353-360.
Walter, M.H., Liu, J.W., Wunn, J., and Hess, D. (1996) Bean ribonuclease-like pathogenesis-related protein genes (Ypr10) display complex patterns of developmental, dark-induced and exogenous-stimulus-dependent expression. Eur. J. Biochem. 239: 281-293.
Wang, C.S., Huang, J.C., and Hu, J.H. (1999) Characterization of two subclasses of PR-10 transcripts in lily anthers and induction of their genes through separate signal transduction pathways. Plant Mol. Biol. 40: 807-814.
Wang, C.S., Walling, L.L., Eckard, K.J., and Lord, E.M. (1992) Immunological Characterization of a Tapetal Protein in Developing Anthers of Lilium longiflorum. Plant Physiol. 99: 822-829.
Warner, S.A., Scott, R., and Draper, J. (1992) Characterisation of a wound-induced transcript from the monocot asparagus that shares similarity with a class of intracellular pathogenesis-related (PR) proteins. Plant Mol. Biol. 19: 555-561.
Warner, S.A., Scott, R., and Draper, J. (1993) Isolation of an asparagus intracellular PR gene (AoPR1) wound-responsive promoter by the inverse polymerase chain reaction and its characterization in transgenic tobacco. Plant J. 3: 191-201.
Warner, S.A., Gill, A., and Draper, J. (1994) The developmental expression of the asparagus intracellular PR protein (AoPR1) gene correlates with sites of phenylpropanoid biosynthesis. Plant J. 6: 31-43.
Weiss, D., and Ori, N. (2007) Mechanisms of cross talk between gibberellin and other hormones. Plant Physiol. 144: 1240-1246.
Wu, F., Yan, M., Li, Y., Chang, S., Song, X., Zhou, Z., and Gong, W. (2003) cDNA cloning, expression, and mutagenesis of a PR-10 protein SPE-16 from the seeds of Pachyrrhizus erosus. Biochem. Biophys. Res. Commun. 312: 761-766.
Wu, H.M., and Cheun, A.Y. (2000) Programmed cell death in plant reproduction. Plant Mol. Biol. 44: 267-281.
Xiong, L., and Yang, Y. (2003) Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell 15: 745-759.
Yamamoto, M., Torikai, S., and Oeda, K. (1997) A major root protein of carrots with high homology to intracellular pathogenesis-related (PR) proteins and pollen allergens. Plant Cell. Physiol. 38: 1080-1086.
Yan, Q., Qi, X., Jiang, Z., Yang, S., and Han, L. (2008) Characterization of a pathogenesis-related class 10 protein (PR-10) from Astragalus mongholicus with ribonuclease activity. Plant Physiol. Biochem. 46: 93-99.
Yu, X., Ekramoddoullah, A.K., and Misra, S. (2000) Characterization of Pin m III cDNA in western white pine. Tree Physiol. 20: 663-671.
Zamani, A., Sturrock, R.N., Ekramoddoullah, A.K., Liu, J.J., and Yu, X. (2004) Gene Cloning and Tissue Expression Analysis of a PR-5 Thaumatin-Like Protein in Phellinus weirii-Infected Douglas-Fir. Phytopathology 94: 1235-1243.
Zhu, Y., Dun, X., Zhou, Z., Xia, S., Yi, B., Wen, J., Shen, J., Ma, C., Tu, J., and Fu, T. (2010) A separation defect of tapetum cells and microspore mother cells results in male sterility in Brassica napus: the role of abscisic acid in early anther development. Plant Mol. Biol. 72: 111-123.


QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top