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研究生:陳妍蓁
研究生(外文):YEN-JEN CHEN
論文名稱:精油化粧品之防腐效能評估
論文名稱(外文):Assessments of preservative efficiency of cosmetics with essential oils
指導教授:蔡玫琳
指導教授(外文):Mei-Lin Tsai
學位類別:碩士
校院名稱:嘉南藥理科技大學
系所名稱:化妝品科技研究所
學門:民生學門
學類:美容學類
論文種類:學術論文
論文出版年:2011
畢業學年度:99
語文別:中文
論文頁數:90
中文關鍵詞:精油防腐試驗化粧品劑型迷迭香檸檬香茅
外文關鍵詞:essential oilsCymbopogon citratesRosmarinus officinalis L.challenge testformulation
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微生物的存在會造成化粧品品質劣變,構成消費者使用的風險,因此化粧品中需添加防腐劑。但消費者使用化粧品後產生過敏反應,推測許多的可能是因為防腐劑的添加所造成的。因此,本研究尋求天然的精油來代替。
本研究探討迷迭香(Rosmarinus officinalis L.)與檸檬香茅(Cymbopogon citratus)精油依不同比例添加於成品中,測其於配方中之防腐效能,並使用GC/MS分析此二種精油的成份。結果顯示,迷迭香精油主成分是以1,8-cineole (24.70%)為主,檸檬香茅精油則是geranial (17.68%)、cis-citral (15.06%)含量較多。在防腐效能試驗部分,兩種精油以單獨添加,或是依同等比例混合,對化粧品不得驗出之大腸桿菌、金黃色葡萄球菌及綠膿桿菌皆具有不錯的防腐效果,對黑黴菌的防腐效果以檸檬香茅精油較佳,單獨使用低濃度添加於成品中即具有很好的防腐效果,迷迭香精油需與之混合使用亦具有不錯的防腐效果。
綜合論文研究結果,精油可以單獨添加或是合併使用於成品中以達防腐效果,具開發潛力。
Microorganism contamination is one of the most important problems in cosmetic industry, and microorganism lead to product degradation or, in the case of pathogens, constitute a risk for the health of the consumer. Therefore, preservatives inhibiting the growth of microorganisms are added to a product, but allergies after using cosmetics occur more and more frequently, which is quite often associated with the presence of synthetic preservatives. For this reason, this study was to verify the antimicrobial activity of essential oils as the components of preservative.
The aim of the study was to test preservative effectiveness of Rosmarinus officinalis L. and Cymbopogon citratus essential oils alone or combination in the formulation during the challenge test. The compositions of the two essential oils, were analyzed by GC/MS. The main components of Rosmarinus officinalis L. essential oil is 1,8-cineole (24.70%). Cymbopogon citratus essential oils contains geranial (17.68%) and cis-citral (15.06%).
In the challenge test, all formulation had preservative abilities against
Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. However, all formulations had preservative abilities against Aspergillus niger administered alone or in combination of Cymbopogon citratus essential oil.
In summary, the essential oils can be added individually or in com- bination into cosmetics to achieve the preservative effect.
摘要......................................................................... I
Abstract ....................................................................II
誌謝 ....................................................................... IV
目錄 ....................................................................... VI
圖目錄 ..................................................................... IX
表目錄 ..................................................................... XI
縮寫表 .................................................................... XII
第一章
前言 ........................................................................ 1
一、 研究動機與目的.......................................................... 2
二、 實驗架構 ............................................................... 4
第二章 文獻回顧 ............................................................. 5
一、精油簡介 ................................................................ 5
(一)精油特性 ................................................................ 5
(二)精油之抗菌活性 .......................................................... 8
二、植物簡介 ............................................................... 18
(一)迷迭香 ................................................................. 18
(二)檸檬香茅 ............................................................... 20
三、防腐劑 ................................................................. 23
(一)化粧品汙染的來源 ....................................................... 23
(二)防腐劑具備條件及種類 ................................................... 25
(三)影響防腐劑效果之因素 ................................................... 26
(四)防腐效能試驗之比較 ..................................................... 27
(五)防腐劑對人體之危害 ..................................................... 30
四、劑型對防腐效能的影響 ................................................... 31
第三章 實驗材料與方法....................................................... 33
一、實驗材料 ............................................................... 33
(一)植物來源 ............................................................... 33
(二)培養基 ..................................................................33
(三)藥品 ................................................................... 34
(四)菌種 ................................................................... 35
(五)儀器設備 ............................................................... 35
二、實驗方法 ............................................................... 36
(一) 配方調製 .............................................................. 36
(二)抗菌活性試驗............................................................ 39
(三)精油成分分析............................................................ 41
三、統計分析 ............................................................... 42
第四章、結果 ............................................................... 43
一、精油成分分析 ........................................................... 43
(一)迷迭香 ................................................................. 43
(二)檸檬香茅 ............................................................... 51
圖 四、檸檬香茅精油層析圖 .................................................. 51
二、精油防腐效能試驗 ....................................................... 55
(一)化粧水 ................................................................. 55
(二) O/W乳液 ............................................................... 61
(三) W/O乳液 ............................................................... 67
第五章、討論 ............................................................... 73
第六章 結論 ................................................................ 75
第七章 參考文獻 ............................................................ 76
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