臺灣博碩士論文加值系統

光學同調斷層掃描（OCT）成像已被證實在透視結構影像上具有潛力，並有機會運用在臨床前和臨床診斷應用。而OCT的特點在於獲得高解析度解剖結構影像，但獲得結構中得血管影像仍是一大困難。螢光顯微鏡是目前在擷取分析螢光染劑注入動物體內血管研究最常用的工具之一。當整合體內螢光顯微術及OCT掃描技術可以有效利用螢光來展示微血管結構及OCT結構資訊。本研究的目的在於建立一種演算法來結合體內螢光資訊及OCT三維成像。在我們的實驗設置中，兩種光學技術會分別擷取資訊並各自進行影像處理及分析，最後再將兩種處理完的影像做結合。本論文主要實驗對象為三隻大鼠，大鼠耳朵固定在平台上，而要觀測的耳朵血管會被擺置在光學設備下。在螢光顯微鏡部分，使用FITC通過靜脈注射並將對比度變化進行記錄。而OCT影像在相同位置下拍攝鼠耳血管，並重建三維影像。由結果顯示結合螢光顯微鏡和OCT成像的三維影像，其中在螢光顯微技術，螢光動態對比度增強（33.95%、66.35%、65.1%，分別在三個動物）。未來展望包括將OCT與螢光顯微鏡平台的整合，並做到血管病變探討，如腦腫瘤動物觀察。

Optical coherence tomography (OCT) imaging has been shown its capability in visualizing anatomical structures and has potential preclinical and clinical diagnostic applications. While the strength of OCT is in distinguishing fined and high-resolution anatomical structures, the current OCT imaging is still have challenges in defining microvascular structures. Current optical fluorescent microscopy is one of the most popular tools in vascular studies by intravenously administering florescent dextrans and capturing the fluorescent microscopy in the in-vivo animals. When integrating the in-vivo fluorescent microscopy with OCT may brings benefits to demonstrate OCT imaging with microvascular structures and provide more information from OCT. The purpose of this study is to construct a integrated procedure to obtain in-vivo fluorescent microscopy and in-vivo 3D OCT imaging. In our experimental setup, each of the two optical technology will capture the information and process and analysis image, then combining two image. Three rats with their ears were fixed and the ear vasculature was served as the observed region. During the fluorescent microscope phase, FITC dextrans were injected through IV and the contrast change were recorded. OCT images were colocalized and presented in 3D. Our results demonstrated that integrated fluorescent microscopy and OCT imaging system can be established. In the fluorescent microscopy, dynamic fluorescent contrast enhancement was observed (33.95%, 66.35%, and 65.1% respectively in the three animals). Future developments include the integration of OCT-optical florescent platform and the vascular observation of the diseased animals such as brain tumors.

指導教授推薦書 i
口試委員會審定書 ii
長庚大學博碩士論文著作授權書 iii
誌 謝 i
摘 要 v
Abstract vi
目錄 vii
圖目錄 ix
第一章 緒論 - 1 -
1-1螢光顯影技術概述 - 1 -
1-2光學同調性斷層掃描概述 - 2 -
1-3研究動機 - 4 -
第二章 實驗材料與方法 - 7 -
2-1螢光顯微鏡 - 7 -
2-1-1螢光顯微鏡系統架構 - 7 -
2-1-2螢光顯影技術 - 10 -
2-1-3螢光顯影GUI介面 - 11 -
2-2光學同調性斷層掃描技術 - 11 -
2-2-1 OCT簡介 - 11 -
2-2-2干涉術 - 12 -
2-2-3 Swept source OCT(SS-OCT) - 17 -
2-2-4 SS-OCT GUI介面 - 18 -
2-3實驗動物架構 - 19 -
2-4影像處理 - 20 -
第三章 實驗結果 - 22 -
3-1螢光血管變化 - 22 -
3-2 OCT深層組織結構 - 29 -
3-4螢光結合OCT - 33 -
3-4-1影像結合技術 - 33 -
3-4-2影像結合結果 - 34 -
第四章 討論 - 37 -
4-1實驗結果討論 - 37 -
4-2未來研究目標 - 37 -
結論 - 39 -
參考文獻 - 40 -

圖目錄
圖1.1 螢光漸層圖 - 2 -
圖1.2 螢光顯微鏡系統架設 - 2 -
圖1.3 OCT一維至三維的影像建立 - 3 -
圖1.4 共焦顯微鏡、OCT以及超音波解析度比較 - 4 -
圖2.1 螢光顯微鏡系統 - 9 -
圖2.2 螢光染劑注入血管後圖 - 10 -
圖2.4 SS-OCT系統架設 - 18 -
圖2.5 實驗老鼠置於螢光顯微鏡 - 20 -
圖2.6 影像處理前後圖 - 21 -
圖3.1 螢光染劑注入前後之變化圖，依老鼠a、b、c三次實驗 - 24 -
圖3.2 老鼠a螢光量化 - 26 -
圖3.3 老鼠b螢光量化 - 27 -
圖3.4 老鼠c螢光量化 - 29 -
圖3.5 老鼠耳朵之二維光學同調斷層掃描影像 - 30 -
圖3.6 OCT三維鼠耳架構，依老鼠a、b、c三次實驗 - 31 -
圖3.8 OCT三維斷面資訊圖 - 33 -
圖3.9 螢光結合OCT資訊 - 36 -

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