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The purpose of this research was to investigate the effects of cool temperature on the growth and flowering of seedlings,and to establish a mucropropagation system of Eustoma grandiflorumnn. Seeds of early cvs MOmo nom Homare and Murasaki noe cvs.Yama no Murasaki Murasaki and Sui Bi Jin,and late There was temperature effect of forcing on cvs. "Murasaki noand "Yama no Murasaki" seedling cultured at 20-23℃ for 2-8followed 4 weeks at 15-18℃ enhanced flowering quickly. Cultivars of King of Blue Beauty, Evening Dress and Azuma no Boeere studied for micropropagation.
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