臺灣博碩士論文加值系統

本研究利用低溫電漿改質技術沉積IPA聚合膜或是以氧氣電漿處理於不同的基材上，探討表面親疏水性對於結冷膠固定量的影響，並進行生物相容性試驗，由水接觸角可以知道IPA電漿沉積膜的水接觸角不太受底材種類的影響，大約落在60~70度之間，以氧氣處理的基材水接觸角都可以達到10度以下，使用氧氣處理表面可使表面帶有許多過氧化的官能基，由AFM圖也可以看出粗糙度有明顯的增加，由於結冷膠的內聚力很強塗佈的時候經常無法得到均勻的薄膜，因此表面積與對水的親和增加可以使得結冷膠接觸角下降，讓表面的結冷膠可以均勻的塗佈在表面上也可以讓基材與結冷膠有較好的結合，經由細胞培養試驗以後可以發現IPA電漿鍍膜雖然不能有效提高與結冷膠的結合度，但可以有效的增加細胞貼附。

In this study, we use the low-temperature plasma modification technology to deposite thin film or activate the substrate surface. Explore the impact of plasma treatment on fixing Gellan gum.The water contact angle showed that after IPA plasma treatment the contact angle was improved to 60~70 degree, and after oxygen plasma treatment the contact angle was improved less than 10 degree. and oxygen plasma treatment can let surface connect with peroxide functional groups. The surface properties of TPU, glass, Al thin film were also characterized by electron spectroscopy for chemical analysis and scanning electron microscope. By the AFM images can seen that the roughness has increased after oxygen plasma treated. Because gellan gum has strong cohesion it is hard to get a uniform film on the surface, therefore, increase the surface hydrophilicity can decrease the contact angle of gellan gum, then we can get good combination of gellan gum with the substrate and uniform coating thin film on the surface. With the cell culture can seem IPA plasma can't effectively improve the combination of substrate and gellan gum but can effectively increase the cell-attached later via cell culture experiments.

總目錄
摘要 I
ABSTRACT II
誌謝 IV
總目錄 V
圖目錄 VIII
表目錄 X
第一章、緒論 1
1.1 研究動機與目的 2
第二章、文獻回顧 4
2.1 彈性體熱可塑性聚氨酯(TPU)彈性體 4
2.2 表面改質技術 6
2.3 電漿表面改質技術 7
2.3.1 低溫電漿處理技術： 10
2.3.2 低溫電漿聚合設備 13
2.3.3 低溫電漿技術佔有的優勢與應用 15
2.4 結冷膠之介紹 17
2.4.1 結冷膠發展歷史 17
2.4.3 結冷膠的基本特性 19
第三章、實驗與分析 21
3.1 實驗流程 21
3.2 實驗基材與藥品 22
3.2.1 基材： 22
3.2.2 電漿反應氣體 23
3.2.3 生物分解性高分子 23
3.3 實驗方法與步驟 24
3.3.1 異丙醇(IPA)電漿處理及氧氣電漿處理 24
3.3.2 浸泡結冷膠溶液 25
3.4分析 26
3.4.1 表面濕潤性量測(water contact angle) 26
3.4.2 原子力掃描探針顯微鏡(AFM) 28
3.4.3 表面形貌觀察(Scanning Electron Microscope；SEM) 30
3.5 細胞培養 31
3.5.1 實驗藥品 31
3.5.2 實驗儀器 31
3.5.3 實驗用細胞株（購自食工所） 32
3.5.4 實驗方法 33
3.5.5 MTT assay 35
第四章、結果與討論 37
4.1 表面濕潤性 37
4.2 時效 42
4.3 表面形貌觀察SEM 43
4.4 AFM 45
4.5 細胞培養測試 53
第五章、結果與討論 58
第六章、参考文獻 59


圖目錄
Fig 2. 1壓力對電子溫度、氣體溫度、離子溫度之關係(YU) 9
Fig 2. 2惰性氣體電漿處理於材料表面所產生的反應[14] 12
Fig 2. 3非聚合反應性氣體電漿處理於材料表面所產生的反應[14] 12
Fig 2. 4 Gellan Gum Structure [25] 18
Fig 2. 5 Gellan Gum Structure [25] 18
Fig 2. 6 結冷膠的分子型態變化[27] 20
Fig 3. 1異丙醇電漿表面沉積設備示意圖。 25
Fig 3. 2氧氣電漿表面處理設備示意圖。 25
Fig 3. 3表面自由能量測Contact angle 27
Fig 3. 4 AFM表面粗糙度之示意圖 30
Fig 3. 5人類皮膚纖維母細胞 32
Fig 3. 6 MTT assay 36
Fig 4. 1TPU，鋁箔，玻璃經IPA電漿處理瓦數對水接觸角的影響 39
Fig 4. 2TPU，鋁箔，玻璃經IPA電漿處理單體量對水接觸角的影響 39
Fig 4. 3TPU，鋁箔，玻璃經IPA電漿處理時間對水接觸角的影響 40
Fig 4. 4玻璃，TPU，鋁箔經不同處理水接觸角變化 41
Fig 4. 5 TPU，鋁箔與玻璃經過氧氣電漿處理後的時效變化 42
Fig 4. 6 SEM (a) (b)0.75wt% 結冷膠烘乾得到的薄膜。 44
Fig 4. 7 SEM plasma改質及塗佈Gel Gum表面形貌(a)未處理TPU (b)經IPA電漿處理(c)經氧氣電漿處理(d) IPA/Gel Gum (e) O2/Gel Gum44
Fig 4. 8 glass未處理之AFM圖 46
Fig 4. 9 glass經IPA電漿處理30S之AFM圖 47
Fig 4. 10 glass經IPA電漿處理30S一個月後之AFM圖 48
Fig 4. 11 glass經IPA電漿處理1min之AFM圖 49
Fig 4. 12 glass經IPA電漿處理5min之AFM圖 50
Fig 4. 13 glass經IPA電漿處理10min之AFM圖 51
Fig 4. 14 CCD966SK fibroblasts分盤後的生長情形 53
Fig 4. 15鋁箔經不同處理後細胞貼附百分比與改質膜表面之關係 55
Fig 4. 16玻璃經不同處理後細胞貼附百分比與改質膜表面之關係 56
Fig 4. 17 TPU經不同處理後細胞貼附百分比與改質膜表面之關係 56
Fig 4. 18 GG經不同處理後細胞貼附百分比與改質膜表面之關係圖 57



表目錄
Table1 表面親水化方法 5
Table2. 1典型熱電漿與低溫電漿之基本特性比較 9
Table2. 2低溫電漿技術在產業中可能的應用 16
Table2. 3結冷膠的特性[26] 19
Table3. 1 結構式 24
Table3. 2 AFM表面粗糙度之定義 29

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