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研究生:蘇宥樺
研究生(外文):Yu-Hua Chueh
論文名稱:在人類真皮纖維母細胞紫外光B照射誘發的細胞傷害sulforaphane具保護作用
論文名稱(外文):Sulforaphane protects human dermal fibroblast from ultraviolet (UV) light B induce cell damage
指導教授:闕小輝
指導教授(外文):Sheau-Huei Chueh
口試委員:劉佩珊王正康
口試委員(外文):Pei-Shan LiuJehng-Kang, Wang
口試日期:2014-05-23
學位類別:碩士
校院名稱:國防醫學院
系所名稱:生物化學研究所
學門:生命科學學門
學類:生物化學學類
論文種類:學術論文
論文出版年:2014
畢業學年度:102
語文別:中文
論文頁數:34
中文關鍵詞:胡蘿蔔素
外文關鍵詞:sulforaphane
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在本論文中我們發現在人類真皮層纖維母細胞中紫外光B照射20 mJ/cm2會造成細胞貼盤數下降,細胞內reactive oxygen species (ROS)增加,細胞死亡增加,以及使fibronectin表現下降,而給予紫外光B照射同時也給sulforaphane的組別對以上現象均能抑制。紫外光B會造成Nuclear erythroid 2-related factor 2 (Nrf2)之表現下降,sulforaphane會增加Nrf2的表現以其及下游目標蛋白,如heme oxygenase-1 (HO-1) 及NAD(P)H quinone oxidoreductase1 (NQO1)表現,進而消除紫外光B誘發的細胞內ROS的增加而可以保護細胞。給予紫外光B同時給予N-acetylcysteine (NAC)也可抑制紫外光B造成的ROS增加,進而保護細胞,使紫外光B造成細胞死亡數減少。所以綜合所有結果在人類真皮層纖維母細胞中,sulforaphane 藉由增加Nrf2表現,而抑制紫外光B造成的ROS增加,進而保護纖維母細胞。
In this study we found that ultraviolet B exposure can decrease adhesion cell number, increase intracellular ROS and induce cell death . In addition, UVB can decrease fibronectin protein expression. All of these effect induce by UVB can be blocked by Sulforaphane. UVB radiation decrease Nrf2 protein expression and sulforaphane can reverse it and inhibit Nrf2 downstream targeting genes expression such as HO-1 and NQO1. By increasing antioxidant gene expression sulforaphane can decrease intracellular ROS and protect cells. Similarly N-acetylcysteine can decrease intracellular ROS and protect cells from UVB.
Taken together, we demonstrate that sulforaphane protect cell from UVB induced damage by activating Nrf2.
目錄
目錄…………………………………………………………………………………………………………………………………………… 1
縮寫表………………………………………………………………………………………………………………………………………3
中文摘要…………………………………………………………………………………………………………………………………4
英文摘要…………………………………………………………………………………………………………………………………5
第一章 緒論………………………………………………………………………………………………………………………6
第一節 紫外光 ( ultraviolet light )………………………………………………………6
第二節 活性氧物質 (reactive oxygen species, ROS)……………………………7
第三節 胡蘿蔔素Sulforaphane…………………………………………………………………………………8
第二章 實驗材料與方法……………………………………………………………………………………………………10
第一節 實驗材料……………………………………………………………………………………………………………………10
第二節 實驗方法……………………………………………………………………………………………………………………14
第三章 實驗結果……………………………………………………………………………………………………………………18
第一節 human dermal fibroblast細胞型態觀察…………………………………………………18
第二節 細胞內活性氧分子(ROS)的變化………………………………………………………………………………19
第三節 探討細胞內活性氧分子在給與UVB下造成細胞死亡的角色……………………………………20
第四節 探討Nrf2在sulforaphane 保護作用的機制…………………………………………………………21
第五節 探討細胞外基質(extracellular matrix,ECM)在給予UVB下降解…………………22
第四章 討論………………………………………………………………………………………………………………………………23
第五章 結論……………………………………………………………………………………25
第六章 參考文獻…………………………………………………………………………………32

圖文目錄
圖一 sulforaphane與UVB對Human dermal fibroblast的影響 …………………………………………26
圖二 sulforaphane與UVB對Human dermal fibroblast中ROS的影響…………………………………27
圖三 NAC與UVB對Human dermal fibroblast中ROS的影響……………………………………………………28
圖四 sulforaphane、NAC與UVB對Human dermal fibroblast死亡的影響………………………29
圖五 sulforaphane與UVB對Human dermal fibroblast中antioxidant enzymes的影響………30
圖六 sulforaphane與UVB對Human dermal fibroblast細胞外基質的影響…………………………………………31
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