臺灣博碩士論文加值系統

1.釘地蜈蚣抗過敏、抗發炎及抗細胞增殖活性成分之研究
由七指蕨科 (Helminthostachyaceae) 植物釘地蜈蚣甲醇粗抽物之正己烷、氯仿分劃中，單離出一個混合物及四個化合物：ß-sitosterol & stigmasterol之混合物 (I)、hexadecanoic acid (II)、tetracosanoic acid (III)、ugonin H (IV) 及ugonin I (V)。Ugonin H (IV) 及ugonin I (V) 為首次從自然界分離得到的新化合物。
經抗細胞增殖試驗結果發現ugonin H (IV) 及ugonin I (V)有明顯抑制U937血癌細胞增殖活性。
而各化合物之抗過敏與抗發炎試驗仍在進行中。
2. 引藻細胞分化誘導及抗細胞增殖之活性成分研究
酯分劃中，單離出一個化合物及一個混合物：bis-(2-ethylhexyl) phthalate (CPA-1) 及hexadecanoic acid和octadecanoic acid之混合物 (CPA-2)。以上化合物均為已知，但都是第一次由引藻中分離得到。
經細胞分化和抗細胞增殖試驗發現bis-(2-ethylhexyl) phthalate (CPA-1) 具有抑制U937血癌細胞增殖之活性 (IC50＝58.4 μg/ml)。

1. Studies on Antiallergic, Antiinflammatory and Antiproliferation Constituents of Helminthostachys zeylanica (L) Hook.
Four compounds and one mixure ――ß-sitosterol and stigmasterol (I), hexadecanoic acid (II), tetracosanoic acid (III), ugonin H (IV) and ugonin I (V), have been isolated from the n-hexane and chloroform fractions of the methanolic extract of Helminthostachys zeylanica (L) Hook. Among the isolated compounds, ugonin H (IV) and ugonin I (V) are isolated for the first time.
The results of the biological activities showed ugonin H (IV) and ugonin I (V) exhibited antiproliferation activities on U937 cells.
However, other compounds are still under test.
2. Studies on Differentiation-induced and Antiproliferation Constituents of Cryptomonadales
One compound and one mixture――bis-(2-ethylhexyl) phthalate (CPA-1), mixture of hexadecanoic acid and octadecanoic acid (CPA-2) have been isolated from the n-hexane, chloroform and ethyl aceteate fractions of the ethyl acetic extract of Cryptomonadales. These compounds were isolated for the first time from Cryptomonadales.
The results of the biological activity investigation showed bis-(2-ethylhexyl) phthalate (CPA-1) exhibited antiproliferation activities on U937 cells (IC50＝58.4 μg/ml).

第一部份 釘地蜈蚣抗過敏、抗發炎及抗細胞增殖活性成分之研究
目錄
英文摘要
中文摘要
第一章 緒論
第一節 研究動機和目的…………………………………………..1
第二節 釘地蜈蚣之藥用植物學考察……………………………..1
第三節 釘地蜈蚣成分研究概況…………………………………..4
第四節 藥理實驗方法簡介………………………………………..9
第二章 釘地蜈蚣成分之抽取與分離………………………………...12
第三章 釘地蜈蚣成分之化學結構鑑定……………………………...14
第四章 粗抽物與成分活性探討
第一節 抗發炎、抗過敏之活性…………………………………29
第二節 抗細胞增殖活性…………………………………………32
第五章 結論 …………………………………………………...35
第六章 實驗部分
第一節 儀器與試藥………………………………………………36
第二節 成分抽取與分離方法……………………………………39
第三節 藥理實驗方法……………………………………………43
第四節 化合物之性質與光譜數據………………………………45
參考文獻………………………………………………………………50
第二部分 引藻細胞分化誘導及抗細胞增殖之活性成分研究
目錄
英文摘要
中文摘要
第一章 緒論
第一節 研究背景與目的…………………………………………...1
第二節 藥理試驗方法簡介………………………………………...2
第二章 引藻成分之抽取與分離………………………………………..3
第三章 引藻成分之化學結構鑑定……………………………………..4
第四章 粗抽物與活性成分探討………………………………………..6
第五章 結論……………………………………………………………10
第六章 實驗部分
第一節 儀器與試藥……………………………………………….11
第二節 成分抽取與分離方法…………………………………….14
第三節 藥理實驗方法…………………………………………….16
第四節 化合物之性質與光譜數據……………………………….18
參考文獻………………………………………………………………..19

第一部份 釘地蜈蚣抗過敏、抗發炎及抗細胞增殖活性成分之研究
1. 甘偉松編著, 藥用植物學, 112頁, 國立中國醫藥研究所, 1996。
2. 許鴻源, 台灣地區出產中藥藥材圖鑑, 11頁, 行政院衛生署中醫藥委員會出版, 1972。
3. 甘偉松編著, 台灣藥用植物誌(卷上), 11頁, 國立中國醫藥研究所, 1979。
4. 邱年永、張光雄編著, 原色台灣藥用植物圖鑑(3), 18頁, 南天圖書有限公司, 1992。
5. T. Murakami, M. Hagiwara, K. Tanaka, and C. M. Chen, Chem. Pharm. Bull.21, 1849, 1851. (1973)
6. 李立聰, 化學, 1, 4. (1978)
7. 謝增華, “錫蘭七指蕨之成分研究”, 國立清華大學化學所碩士論文。(1990)
8. Makishima, M.; Kanatani. Y.; Yamamoto-Yamaguchi, Y.; Honma, Y. Enhancement of activity of alpha, 25-dihydroxyvitamin D3 for growth inhibition and differentiation induction of human myelomonocytic leukemia cells by tretinoin tocoferil, an alpha-tocopherol ester of all-trans retinoic acid. Blood, 87, 3384-3394. (1996)
9. He, Q.; Jiang, D. A novel aminosteroid is active for proliferation inhibition and differentiation induction of human acute myeloid leukemia HL-60 cells. Leuk. Res. 23, 369-372. (1999)
10. Berridge, M. V.; Tan, A. S. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvment of mitochondrial electron transport in MTT reduction. Aech. Biochem. Biophs.303,474-482. (1993)
11. Ishiyama, M.; Tominaga, H.; Shiga, M.; Sasamoto, K.; Ohkura, Y.; Ueno, K.; Watanabe, M. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a soluble formazam dye. In Vitro Toxicol. 8,187-190. (1995)
12. Pradhan, B. P.; Chakraborty, D. K.; Subba, G. C., A triterpenoid lactone form Leucas aspera, Phytochemistry, 29, 1693-1695. (1990)
13. Huang, K. F.; Yen, Y. F., Constituents of Erythrina variegata; Chin. Pharm. J., 49, 21-29. (1997)
14. Gan, K. H. Studies on the cytotoxic and antihepatotoxic agents from Formosan Natural Resources. Ph.D. Thesis; Graduate Institute of Pharmaceutical Sciences, Kaohsiung Medical College. (1995)
15. Huang, K. F.; Yen, Y. F., Constituents of Roots of Elaeagnus glabra;Chin. Pharm. J., 47, 493-500. (1995)
16. Sasaki, S. I., Handbook of Proton-NMR Spectra and Data, Academic Press, Vol. 5, 190. (1985-1986)
17. Sasaki, S. I., Handbook of Proton-NMR Spectra and Data, Academic Press, Vol. 5, 289. (1985-1986)
18. 姚新生編著, 天然藥物化學第二版, 206頁, 人民衛生出版社, 1994。
19. McClain, D. E.; Donton, M. A., Chock, S. and Catravas, C. N, Biochim. Acta, 193 (1989)
20. Smith,R. J.; Iden, S. S., Biochim. Biophys. Res. Commun. 91, 263-271. (1979)
第二部分 引藻細胞分化誘導及抗細胞增殖之活性成分研究
1. Makishima, M.; Kanatani. Y.; Yamamoto-Yamaguchi, Y.; Honma, Y. Enhancement of activity of alpha, 25-dihydroxyvitamin D3 for growth inhibition and differentiation induction of human myelomonocytic leukemia cells by tretinoin tocoferil, an alpha-tocopherol ester of all-trans retinoic acid. Blood, 87, 3384-3394. (1996)
2. He, Q.; Jiang, D. A novel aminosteroid is active for proliferation inhibition and differentiation induction of human acute myeloid leukemia HL-60 cells. Leuk. Res. 23, 369-372. (1999)
3. Berridge, M. V.; Tan, A. S. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvment of mitochondrial electron transport in MTT reduction. Aech. Biochem. Biophs. 303, 474-482. (1993)
4. Ishiyama, M.; Tominaga, H.; Shiga, M.; Sasamoto, K.; Ohkura, Y.; Ueno, K.; Watanabe, M. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a soluble formazan dye. In Vitro Toxicol. 8, 187-190. (1995)
5. Kawaii, S.; Tomono, Y.; Katase, E.; Ogawa, K.; Yano, M.; Takemura, Y.; Juichi, M.; Ito, C.; Furukawa, H. Acridones as inducers of HL-60 cell differentiation. Leuk. Res. 23, 263-269. (1999)
6. Weast, R, C.; Grassell, J. G. Handbook of Data on Organic Compounds Vol 2; CRC Press : Florida, USA., 785. (1989)
7. Hayashi, S.; Asakawa, Y.; Ishida, T. Phthalate Esters of Cryptotaenia canadensis DC. var. japonica Makino (Umbelliferae). Tetrahedron Letters, 5061. (1967)
8. Sasaki, S. I., Handbook of Proton-NMR Spectra and Data, Academic Press, Vol. 5, 190. (1985-1986)
9. S. I. Sasaki, Handbook of Proton-NMR Spectra and Data, Academic Press, Vol. 5, 269. (1985-1986)