臺灣博碩士論文加值系統

水楊酸(Salicylic acid，SA)為普遍植物中常見的的激素，又因為有良好的生物相容性、抗菌力、消炎等功效，SA被大量應用在藥物與生活用品中。首先，本研究針對SA、水楊酸甲酯(methyl salicylate, MeSA)、茉莉酸(jasmonic acid, JA)等目標物分子，探討毛細管電泳(Capillary electrophoresis, CE)連接紫外光偵測器(ultraviolet detector, UV)之最佳化分離參數（前導溶液：400 mM Tris Borate buffer (pH 10.0), 100 mM SDS, 15% Acetonitrile，分離溶液：100 mM Tris borate buffer (pH 9.0), 100 mM sodium dodecyl sulfate (SDS), 15% Acetonitrile, 0.3% poly(ethylene oxide) (MW: 8M)，分離電壓為10 kV）。同時利用毛細管電泳線上濃縮機制降低此三種物質的偵測極限，分別為SA: 3.2310-8 M ；MeSA: 9.8410-8 M；JA: 6.3510-7 M。其濃縮倍率最高可提升至131.4倍。其濃縮機制為大體積之分析物下於高黏度聚合物之界面進行堆積作用，使濃縮倍率隨之提升。為了更進一步提高偵測靈敏度，在本研究的前處理階段，針對目標分析物SA開發出一套前處理模式，以氧化鐵奈米粒子對SA獨特的選擇性，成功於真實樣品中純化並濃縮SA。結合此前處理方法，其濃縮倍率可至1120倍，SA之偵測極限為3.7910-9 M。利用此分析方法可偵測正常的圓葉菸草葉片(Nicotiana benthamiana)與受台灣番茄捲葉病毒(Nicotiana benthamiana-ToLCTWV)感染的圓葉菸草葉片上的SA含量。初步結果發現，受台灣番茄捲葉病毒感染的菸草其SA濃度較正常的菸草葉片低(81.5%)。本方法也可應用於治療疣症的市售藥膏與市售洗髮精中SA含量分析，其回收率為93.6%-102.7%。

Salicylic acid (SA) is a plant hormone which possesses biocompatibility, antibacterial activity, anti-inflammation ability, thus SA was used widely in our living life. My study is focused on determination of SA, methyl salicylate (MeSA), jasmonic acid (JA) by using capillary electrophoresis (CE) with ultraviolet detection. Under optimum conditions (leading buffer: 400 mM Tris Borate (TB) buffer (pH 10), 100 mM SDS, 15% ACN; running buffer: 100 mM TB buffer (pH 9), 100 mM sodium dodecyl sulfate (SDS), 15% acetonitrile(ACN), 0.3% poly(ethylene oxide, PEO) (MW: 8M)), the limit of detection (LOD, S/N =3) for JA, SA, and MeSA was 0.635, 0.0323 and 0.0986 µM, respectively. The enhance factors were achieved to 36.2 to 131 folds comparing to separation without stacking technique. The stacking mechanism by the proposed method is based on the mobility of analyst slowed down in the interface between high viscosity of PEO solution and background electrolyte, thus analyst got concentration. Further to improve detection sensitivity of SA, magnetic Fe3O4 nanoparticles were used to selectively extract SA. As we known, the enediol group of the SA coordinated to Fe3+ ions on the Fe3O4 nanoparticles surface, leading to the formation of Fe3+-SA coordination compounds. Combining CE on-line concentration and Fe3O4 nanoparticle based extraction, sensitivity enhancement for SA was achieved to 1119 folds with LOD (S/N =3) as low as 3.79 nM. This method has the potential to be an effective analytical tool for SA in different tobacco leaves, shampoo and ointment. The recoveries for real samples (tobacco leaves, shampoo and ointment) were ranged from 92.8-105.2%.

目錄
中文摘要……………………………………………………………………………………1
英文摘要……………………………………………………………………………………2
目錄…………………………………………………………………………………………3
圖目錄………………………………………………………………………………………5
表目錄………………………………………………………………………………………6
第一章 緒論………………………………………………………………………………7
1.1 前言…………………………………………………………………………7
1.2 水楊酸、水楊酸甲酯、茉莉酸的介紹………………………………………7
1.2.1 水楊酸……………………………………………………………7
1.2.2 水楊酸甲酯………………………………………………………8
1.2.3 茉莉酸…………………………………………………………8
1.3 毛細管電泳………………………………………………………………9
1.3.1 分離原理………………………………………………………10
1.3.2 影響分離因素…………………………………………………13
1.3.3 線上濃縮機制…………………………………………………13
1.4 萃取方式…………………………………………………………………14
1.5 氧化鐵奈米粒子…………………………………………………………16
1.6 研究動機…………………………………………………………………17
第二章 實驗………………………………………………………………………………18
2.1 儀器…………………………………………………………………………18
2.2 材料…………………………………………………………………………18
2.3 藥品…………………………………………………………………………18
2.4 溶液的配置…………………………………………………………………19
2.5 毛細管處理方法……………………………………………………………19
2.6 氧化鐵奈米粒子的合成……………………………………………………19
2.7 真實樣品前處理……………………………………………………………20
2.8 萃取步驟……………………………………………………………………20
第三章 結果與討論………………………………………………………………………21
3.1 毛細管電泳分離三種植物激素的探討…………………………………21
3.2 毛細管電泳線上濃縮最佳化探討………………………………………23
3.3 萃取最佳化條件探討……………………………………………………29
3.4 植物樣品的應用…………………………………………………………37
3.5 市售樣品的應用…………………………………………………………42
第四章 結論及未來展望…………………………………………………………………43
參考資料…………………………………………………………………………………44

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