臺灣博碩士論文加值系統

白殭菌素已知為環狀胜肽毒素之一，主要為白殭菌生合成產物。本研究以農業藥物毒物試驗所提供之白殭菌Beauveria bassiana A1為培養菌株，嘗試利用不同培養基進行液態發酵培養白殭菌生合成其代謝物-白殭菌素，初步結果顯示以25 g/L sucrose、25 mL/L edible molasses、10 g/L malt extract、10 g/L NZ broth、2 g/L K2HPO4為培養基質時，幾乎無白殭菌素之產生，但若以玉米水萃取液為基礎培養基質，再添加上述培養基質濃度，基質起始調配pH值為6.2，在25 ℃下以150 rpm振盪培養76 h後，可得到本實驗室目前最佳白殭菌素產量為699μg/L，由此可知玉米水萃取液為影響白殭菌生合成白殭菌素的重要組成分之一。

Beauvericin, one of cyclodepsipeptide toxins, is one kind of metabolic products of Beauveria species. The preliminary culture media were studied for beauvericin production of Beauveria bassiana A1, kindly donated by Professor Suey-Sheng kao at the Biopesticide Department, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, in liquid culture. There was much little beauvericin production with the culture medium composed of sucrose (25g/L) , edible molasses (25mL/L) , malt extract (10g/L) , NZ broth (10g/L) , k2HPO4 (2g/L) . The aqueous (water) extract liquid of corn was as a basic medium. When the medium positions described above were dissolved in the water extract liquid, which was as the culture medium, the beauvericin production was 699 μg/L for 76h shaken culture (150rpm) at 25℃ and the initial pH6.2 of the medium. The corn water extract liquid becomes a suitable source and represents a potent role for B. bassiana A1 in beauvericin production.

目 錄
頁次
封面內頁
簽名頁
授權書 iii
中文摘要 iv
英文摘要 v
致謝 vi
目錄 vii
圖目錄 ix
表目錄 x
第一章 緒論 1
第二章 文獻回顧 4
2.1蟲生真菌的分類 4
2.2白殭菌屬分種 8
2.3白殭菌殺蟲原理 10
2.4白殭菌之寄生範圍及標的害蟲 12
2.5微生物農藥 12
2.6白殭菌素 15
2.7農用抗生素 16
2.8胜肽類抗生素 17
2.9 HPLC之儀器原理 19
第三章 材料與方法 21
3.1實驗材料 21
3.2儀器設備 21
3.3實驗方法 22
3.3.1培養方法 22
3.3.2白殭菌素以HPLC定量分析 23
第四章 結果與討論 28
4.1培養基之最適化研究 28
4.2最適玉米之測試 28
4.3最適玉米水萃取液之測試 29
第五章 結論與展望 33
參考文獻 34
附錄 43
圖目錄
圖2.1 毒素之環狀結構 11
圖2.2 白殭菌素之化學結構式 13
圖2.3 生物農藥的分類 14
圖3.1 白殭菌素標準品之全波長 25
圖3.2 白殭菌素標準品之高效液相層析圖 26
圖3.3 白殭菌素標準品的校正曲線 27
圖4.1 NZ Broth對白殭菌素產量的影響 30
圖4.2 玉米水萃取液對白殭菌素產量的影響 31
圖4.3 玉米不同部位對白殭菌素產量的影響 32
表目錄
表2.1 白殭菌產生的幾種重要毒素 18

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