臺灣博碩士論文加值系統

青蔥為臺灣具重要經濟價值之經濟作物。為減輕成本，農民往往採用連作，但產量隨連作次數之增加而減少，且易罹病蟲害，影響甚鉅。連作障礙的發生原因可能是物理性、化學性或生物性的。研究指出青蔥連作使土壤酸化，影響植物營養。施用石灰及有機質肥料有助於改善其連作障礙，然造成青蔥連作障礙原因仍不甚明朗。影響青蔥生長之土壤相關研究僅限於化學性質，未見對其土壤酵素、微生物生態的研究。本研究之目的在探討不同施肥管理對青蔥生長、土壤化學性質、酵素活性及微生物族群結構之影響，以期提供研究青蔥栽種及管理青蔥栽種土壤之參考。青蔥進行兩作栽培試驗，收穫後採植體進行產量與養分分析，收穫時同時採取根圈土壤進行化學性質、酵素活性、微生物生質量及微生物族群分析。試驗於臺灣大學溫室進行，土壤採自宜蘭，共六處理六重複，採完全逢機設計：試驗處理有：（一）對照組（CK），不施任何肥料；（二）化學肥料處理（CF）；（三）有機質肥料氮一倍量處理（1X）；（四）有機質肥料氮二倍量處理（2X）；（五）有機質肥料與化學肥料 各半處理（CO）；（六）有機質肥料氮一倍量並加入苦茶粕處理（TO）。每盆裝10 kg 土壤。化學肥料施用量每盆為1.56-0.65-0.65 g（N-P2O5-K2O）。第二作栽培時未施肥。作物分別於移植後第一百日採收，均採取全株，同時亦採取根圈土壤。試驗結果顯示不同施肥管理對土壤化學、生化性質、酵素活性及微生物族群均產生影響。施用尿素會使青蔥栽培土壤變酸。施用化學肥料之土壤與施用茶渣牛糞堆肥者比，其有機質含量、酵素活性及微生物生質量較低，有較高的真菌/細菌比例，並且特定微生物逆境指標提高。施用茶渣牛糞堆肥時，土壤酸化問題改善，有機質含量較未施用茶渣牛糞堆肥者高，與土壤有機質相關的酵素活性與微生物生質量亦提高。添加苦茶粕於施用茶渣牛糞堆肥之土壤中，有較低之放線菌/細菌比例。各處理第二作後之土壤pH、酵素活性、微生物生質量與微生物族群PLFA濃度均顯著較第一作後者之相同處理者低，其中化學肥料處理之兩作差異最大。第二作後之兩倍有機質肥料、一倍有機質肥料與化學肥料處理土壤中之革蘭氏陽性菌/革蘭氏陰性菌比例顯著較第一作相同處理低，其中化學肥料處理下降26%最多。青蔥第二作收穫後，化學肥料處理土壤放線菌/細菌之 PLFA 比例顯著低於第一作後相同之處理。植體生質量與土壤酵素活性、硝化作用、微生物質生質量與PLFA有極顯著相關。酵素活性彼此間與微生物族群彼此間有顯著相關。第二作化學肥料處理青蔥產量下降幅度達35％。青蔥栽培施用化學肥料，可能會有土壤品質與青蔥產量下降之風險，而施用有機質肥料則有助於維持栽培土壤pH、酵素活性、減輕微生物逆境，增加土壤微生物生質量。

Green onion (Allium fistulosum L.) is an important cash crop in Taiwan. Monocropping of green onion is a common agricultural practice. Howerer, monocropping often resulted in lowing soil quality and increasing pest and disease incidences as well as aridification of the soil. Application of organic matter or lime can alleviate the problem resulting from monocropping. There are studies about the monocropping of green onion on the soil chemical properties. However, the effects of monocropping on soil biochemical properties and microbial properties are need to study. The objective of the present study was to evaluate the effect of different fertilization managements on green onion growth and soil chemical properties, enzyme activities, and microbial communities. The experiment was conducted in a green house in National Taiwan University. The soil used was taken from an green onion cultivation farm land in I-lan. There were six treatments (1) no fertilizer application for control, (CK) (2) applying chemical fertilizer treatment (1.56-0.65-0.65 g pot-1)（N-P2O5-K2O）, (CF) (3) applying organic fertilizer with the same amount N as CF, (1X) (4) applying organic fertilizer with the two-fold amount N as CF, (2X) (5) applying 50% of chemical fertilizer and along with 50% of organic fertilizer mixed, (CO) (6) applying organic fertilizer (same as 1X) and tea seed pomace, (TO). All treatments were replicated six times and arranged in completely radom design. No fertilizer was applied in the second crop. The plants were harvested after 100 days after of transplanting. Soils of root zone were taken after haversting the crops. The plants were subjected chemical analysis for the nutrient contents and the soils was subjected the analysis for chemical properties, enzyme activies, microbial structure using phospholipid fatty acid (PLFA). The results indicated the soil pH, organic matter content, enzyme activities and microbial biomass of chemical fertilizer treated plots were lower than those of organic matter treated plots. The fungi/bacteria ratio and specific microbial stress indicatior of the CF treatment were higher than those of organc fertilizer treatments. The actinomyces/bacteria ratio of the soil of TO treatment was higher than that of organic fertilizer treatment. All enzyme activities studied showed a significant linear correlation with the organic matter cotent of the soil. There were significant correlations between the activities of different soil enzymes. There was a significant difference between the soil pH, enzyme activities, and microbial biomass of treatments between the first and the second crop. After harvesting of the second crop, the Gram positive/negative bacteria ratio of the soil of the 1X, 2X and CF treatment were significantly lower than the soil of the same treatment of the first crop. The Gram positive/negative bacteria ratio of the soil of the CF treatment decreased 26% in the second crop compared with the first crop. After harvesting of the second crop, the actinomyces/bacteria ratio of the soil of the CF treatment was significantly lower than the soil of the same treatment of the first crop. There were significant correlations between green onion biomass, soil biochemical properties, microbial biomass and PLFA. There were significant correlations between green onion biomass, and soil biochemical properties, microbial biomass, and PLFA, respectively. The biomass of second crop green onion deceased 35% compared with the first crop. In conclusion, application of chemical fertilizer may result in a decrease in soil quality and green onion yield. Organic fertilizer resulted in maintaining the soil pH, increasing enzyme activities and microbial biomass of the soil that cultivated green onion, as well as reducing the stress conditions for microbes.

目錄
謝誌 I
摘要 II
Abstract IV
目錄 VI
圖目錄 VII
表目錄 VIII
附錄目錄 IX
前言 1
前人研究 3
材料與方法 12
結果
一、不同施肥管理對青蔥收穫後土壤化學性質之影響 33
二、不同施肥管理對青蔥收穫後土壤生物化學性質之影響 37
三、不同施肥管理對於青蔥收穫後土壤微生物性質之影響 42
四、不同施肥管理對青蔥生長及養分之影響 49
五、青蔥生質量、土壤化學性質、生物化學性質、酵素活性與微生物族群間彼此間相關性 56
討論
一、不同施肥管理對青蔥收穫後土壤化學性質之影響 59
二、不同施肥管理對青蔥收穫後土壤生物化學性質之影響 63
三、不同施肥管理對青蔥收穫後土壤微生物性質之影響 65
四、不同施肥管理對青蔥生長及養分之影響 68
結論 71
參考文獻 72
附錄 96

丁文彥。1993。蘭陽地區青蔥栽培土壤酸化及連作障礙之改進方法。行政院農業委員會花蓮區農業改良場農業彙報，6: 2-4。
王一雄。1997。土壤環境汙染與農藥。國立編譯館，台北，台灣。
王銀波、趙震慶。1995。有機及化肥法下土壤環境與養分收支比較。台灣省政府農林廳，南投，台灣。
王鐘合、林毓雯、林木連、邱麗蓉。2000。有機質肥料在蔬菜栽培上之施用技術，行政院農業委員會，台中，台灣。
行政院農糧署。2009。台灣地區農產品生產量值。行政院農糧署，台北，台灣。
孫星衍。1995。神農本草經。商務印書館，上海，中國。
卓文君。1998。設施葉菜類連作障礙之研究。國立中興大學園藝研究所碩士論文，台中，台灣。
許苑培。1995。蔥植體殘質對青蔥、萵苣與蘿蔔發芽抑制之生物檢定及連作對後作生育與產量之影響。國立中興大學園藝研究所碩士論文，台中，臺灣。
張禮愷。1987。土壤酶學。科學出版社，北京，中國。
陳玉雯。2009。芹菜連作障礙之研究。國立中興大學園藝研究所碩士論文，台中，台灣。
詹朝清、丁文彥、呂文通。1991。腐植酸及有機質肥料對青蔥生長及連作之影響。花蓮區農業改良場彙報，7：133-146。
詹朝清。1995。青蔥連作障礙及葉尖枯萎改進之研究。行政院農委會花蓮區農業改良場研究彙報，15：45-57。
郭孚耀、吳世偉。1988。蔬菜設施栽培連作問題及病蟲害管理。台灣省農業試驗所鳳山熱帶園藝試驗所，高雄，台灣。
曾潤錦，謝昱光。1981。蔬菜園有機質肥料與化學肥料配合試驗。蔬菜作物試驗研究彙報147-145。
楊正偉。2006。苦茶粕防治甘藍幼苗立枯病之效果與其有效成分之鑑定。國立中興大學植物病理研究所碩士論文，台中，台灣。
楊明富。1991。水稻、玉米輪作制度下施用堆肥對土壤肥力及作物產量之影響。國立中興大學碩士論文，台中，台灣。
鄧耀宗、黃伯恩。1993。台灣永續農業之現況與展望。行政院農委會桃園區農業改良場，桃園，台灣。
劉興榮。2009。青蔥栽培管理手冊。行政院農業委員會花蓮區農業改良場，花蓮，台灣。
蔣汝國、郭金條、侯福分。1998。利用菸砂及苦茶粕防除水稻福壽螺可行性之探討。菸試彙報，44：71-79。
簡秀芳、李木川、鄒慧娟、黃德昌。2006。水稻福壽螺之防治。行政院農業委員會，台北，台灣。

賴朝明、洪崑煌。1991。台灣主要耕地土壤尿素酶、酸性磷酸酯酶去氫酶之活性及其與土壤性質的關係。中國農業化學會誌，29：382-395。
賴朝明。1993。不同作物栽培系統對土壤酵素活性的影響。中華生質能源會會誌，12：89-97。
Allen, S. and J. A. C. Smith. 1986. Ammonium nutrition in Ricnus communis: Its effect on plant growth and the chemical compositions of the whole plant, xylem and phloem saps. J. Exp. Bot., 37: 1599-1610.
Alvarez, M. B., S. Gagne, and H. Antoun. 1995. Effect of compost on rhizosphere microflora of tomato and on the incidence of plant growth promoting rhizobacteria. Appl. Environ. Microbial., 61: 194-199.
Anderson, J. P. E. and K. H. Domsch. 1973. Quantification of bacterial and fungal contributions to soil respiration. Arch Mikrobiol., 93: 113-127.
Anderson, T. H. and T. R. G. Gray. 1990. Soil microbial carbon uptake characteristics in relation to soil management. FEMS Microbial. Ecol., 74: 11-20.
Anthonisen, A. C., R. C. Loehr, T. B. S. Prakasam, and E. G. Srinath. 1776. Inhibition of nitrification by ammonia and nitrous acid. J. Wat. Pollut. Control Fed., 48: 835-852.
Asghar, K. M. and Y. Kanehiro. 1980. Effects of sugarcane trash and pineapple residue incorporation on soil pH, redox potential, extractable Al, Fe and Mn. Trop Agric., 57: 245-258.
Ball, D. F. 1964. Loss-on ignition as estimate of organic matter and organic carbon in non0calcareous soils. J. Soil Sci., 15: 84-92.
Bandick, A. K. and R. P. Dick. 1999. Field management effects on soil enzyme activities. Soil Biol. Biochem., 31: 1471-1479.
Barber, S. A. 1995. pp. 398. In Soil Nutrient Bioavailability. Wiley, B. (ed.) New York.
Barker, A. V. and G.M. Bryson. 1999. Nitrogen. pp. 32-39. In A. V. Barker, and D. J. Pobleam (eds.) Handbook of Plant Nutrition. Taylor and Francis, New York, USA.
Batish, D. R., H. P. Singh, R. K. Kohli, and S. Kaur. 2001. Crop allelopathy and its role in ecological agriculture. J. Crop Prod., 4: 121–162.
Bauer, A. and A. L. Black. 1994. Quantification of the effect of soil organic matter content on soil productivity. Soil Sci. Soc. Am. J., 58: 185-193.
Bear, F. E. and S. J. Toth. 1942. Phosphate fixation in soil and its practical control. Indus. Eng. Chem., 34: 49-52.
Bending, G. D., C.Putland, and F. Rayns. 2000. Changes in microbial commumty metabolism and labile organic matter fractions as early indicators of the impact of management on soil biological quality. Biol. Fertil. Soils, 31:78-84.
Berg, P., and P. J. A. Rosswall. 1985. Ammonium oxidizer numbers, potential and actual oxidation rates in two Swedish arable soils. Biol. Fertil. Soils, 1: 131-140.
Blacquiere, T., R. Hofstra, and I. Stolen. 1987. Ammonium and nitrate nutrition in Plantago lanceolate and Plantago major L. ssp. Major. I. Aspects of growth, chemical composition and root respiration. Plant Soil, 104: 129-141.
Blagodatskaya, E. and T. H. Anderson. 1998. E.V. Interactive effects of pH and substrate quality on the fungal-to-bacterial ratio and QCO2 of microbial communities in forest soils. Soil Biol. Biochem., 30: 1269–1274.
Blagodatskaya, E. V., T. V. Pampura, E. G. Demyanova, and T. N. Myakshina. 2006. Effect of lead on growth characteristics of mucroorganisms in soil and rhizosphere of Dactylis glomerata. Eurasian Soil Sci., 39: 653-660.
Bolan, N. S., M. J. Hedley, and R. E. White. 1991. Processes of soil acidification during nitrogen cycling with emphasis on legume based pastures. Plant Soil, 134: 53-63.
Bolton, H., L. F. Jr. Elliot, R. I. Papendick, and D. F. Bezdicek. 1985. Soil microbial biomass and selected soil enzyme activities: Effect of fertilization and cropping practices. Soil Biol. Biochem., 17:297-302.
Bossio, D. A., K. M. Scow, N, Gaunapala, and K. J. Garham. 1998. Determinats of soil microbial communities: effects of agricultural managemwnt, season, and soil type of phospholipid fatty acid profile. Microbial. Ecol., 32: 7-12.
Bossio, D. A. and K. M. Scow. 1998. Impacts of carbon and flodding on soil microbial communities:phospholipid fatty acid profiles and substrate utilization patterns. Microbial. Ecol., 35: 265-278.
Brady, N. C. and R. P. Weil. 1999. Soil organic matter. pp. 446-490. In Brady, N. C. and R. P. Weil (eds.) The Nature and Properties of the Soils. Upper Saddle River, New Jersy, USA.
Breakwell, D. P. and R. F. Turco. 1990. Nutrient and phyto contributions of residues to soil in no-till continuous corn ecosystems. Biol. Fertil., 8: 328-334.
Bremner, J. M. 1965. Inorganic forms of nitrogen. pp. 1149-1178. In Black, C. A. (eds.) Mehtods of Soil Analysis. Part 2. Agro. Madison, Wisconsin, USA.
Bremner, J. M., and C. S. Mulvaney. 1982. Salicy acid-thiosulfate nodification on Kjeldahl method to include nitrite and nitrate. pp. 1149-1178. In Black, C. A. (ed.) Mehtods of Soil Analysis. Part 2. Madison, Wisconsin, USA.
Brennan, P. J. 1988. Mycobacterium and other actinomycetes. pp. 204-298. In Ratledge, C., S G. Wilkinson, (eds.) Microbial Lipids. Academic Press, London, UK.
Breure, A. M. 2005. Ecological soil monitoring and quality assessment. pp. 281-305. In Doelman, P. and H. J. P. Eijsackers (eds.) Vital Soil: Function, Value and Properties. Elsevier, Amsterdam, Nederlands.
Broadbent, F. E. 1948. Nitrogen release and carbon loss from soil organic matter during decomposition of added plant residues. Soil. Sci. Soc. Amer. Proc., 12 :246-254.
Broaddus, G. M., and J. E. Hoseley. 1965. Factors affecting the levels of nitrate nitrogen in cured tobacco leaves. Tob. Sci., 9: 149-157.
Brookes, P. C. 1995. The use of microbial parameters in monitoring soil pollution by heavy metals. Biol. Fertil. Soils, 19: 269-279.
Burket, J. Z. and R. P. Dick. 1998. Microbial and soil parameters in relation to N mineralization in soils of diverse genesis under differing management systems. Biol. Fertil. Soils, 27: 430-438.
Ceccanti, B., B. Pezzarossa, F. J. Gallardo-Lancho, and Gmassciandaro. 1993. Biotest as markers of soil utilization and fertility. Geomicriobial. J., 11: 309-316.
Chakrabarti, K., B. Sarkar, A. Chakraborty, P. Banik, and D. Bagchi. 2000. Organic recycling for soil quality conservation in a sub-tropical plateau region. J. Agron. Crop Sci., 184: 137-142.
Chang, C., T. G. Sommerfeldt, and T. Entz. 1991. Soil chemistry after eleven annual application of cattle feelot manure. J. Environ. Qual., 20: 475-481.
Chapman, H. D. 1966. Diagnostic criteria for plants and soils. pp. 489-499. In Chapman, H. D. (ed.) Univ. of Calif., Riverside, USA.
Charles, A. S. 2007. Phosphorus. pp. 51-90. In Barker, A.V. and D.J. Pilbeam (eds.) Handbook of Plant Nutrition. Taylor & Francis, Boca Raton, London, UK.
Chung, R. S., and F. N. Wang. 2000. Effect of different composts on growth and nitrogen composition of Chinese musted in an acid red soil. Commun. Soil Sci. Plant Anal., 31: 1209-1224.
Clark, F. E. 1949. Soil microorganisms and plant roots. Adv. Agron., 1: 241-288.
Curl, E. A. and P. Truelove. 1986. Introduction. p. 1-8. In D. F. RBommer. (ed). The Rhizosphere. Sringer-Verlog, Berlin, Germany.
Dalal, R. C. 1998. Soil microbial biomass–what do the numbers really mean? Aust. J. Exp. Agric., 38: 649-665.
Deng, S. P. and M. A. Tabatabai. 1996. Effect of tillage and residue management on enzyme activities in soils: II. Glycosidase. Biol. Fertil. Soils, 22: 208–213.
Deng, S. P. and M. A. Tabatabai. 1997. Effect of tillage and residue management on enzyme activities in soils: III. Phosphatases and acrylsulfatase. Biol. Fertil. Soils, 24: 141-146.
Diaz-Marcote, I. and A. Polo. 1995. MSW compost for the restoration of degraded soil. In “World Congress on waste Management. ” Proc. of the 25th Anniversary of the International Solid Waste 15th-20th October, 1995. ISWA, Vienna (CD Rom).
Dick, W.A. 1984. Influence of long-term tillage and crop rotation combinations on soil enzyme activities. Soil Sci. Soc. Am. J., 48: 569-574.
Dick, R. P. 1994. Soil enzyme activities as indicators of soil quality. In Doran, J. W. (ed.) Defining Soil Quality for a Sustainable Environment. Madison, Wisconsin, USA.
Dick, R. P. 1992. A review: Lone-term effects of agriculture systems on soil biochemical microbial parameters. Agri. Ecosy. Environ., 40: 25-36.
Dick, R. P., P. Thomas, and J. J. Halvorson. 1996 Standardized methods, sampling, and pretreatment. pp. 107-121. In W. Doran, and A. J. Jones (eds.) Methods for Assessing Soil Quality. Madison, Wisconsin, USA.
Dick, R P., P. E. Rasmussen, and E. A. Kerle. 1988. Influence of long term residue residue management on soil enzyme activies in relation to soil chemical properties of a wheat-fallow system. Biol. Fertil. Soil, 6: 159-164.
Dick, W.A. and Jr. D. M. Van Doren. 1985. Continuous tillage and rotation combinations effects on corn, soybean, and oat yields. Agron. J., 77: 459-465.
Dodor, D. E. and M. A. Tabatabai. 2000. Effect of cropping systems and microbial biomass on arylamidase activity in soil. Biol Fertil. Soils, 35: 253-261.
Dodor, D. E. and M. A. Tabatabai. 2003. Effect of cropping systems on phosphatases in soils. J. Plant Nutr. Soil Sci., 166: 7-13.
Doran, J. E., D. C. Coleman, D. F. Bezdicek, and B. A. Stewart. 1994. Defining Soil Quality for a Sustainable Enviroment. American Sociaty of Agronomy, Madison, Wisconsin, SA.
Enwall, K., L. Philippot, and S. Halllin. 2005. Activity and composition of the denitrifying bacteria community respond differently to long-term fertilization. Appl. Environ. Microbial., 71: 8335-8343.
Epstein, E. and H. Kohnke. 1957. Soil aeration as affected by organic matter application. Soil Sci. Soc. Am. J., 21: 585-588.
Federle T. W. 1986. Microbial distribution in soil – new techniques. pp. 493-498. In Megusar F. and M. Gantar. (eds.) Perspectives in Microbal Ecology. Slovene Scociety for Microbiology, Ljubjana, Germany.
Feng, Y., A. Motta, D. Reeves, C. Burmester, E. van Santen, and J. Osborne. 2003. Soil microbial communities under conventional- till and no till continuous cotton systems. Soil Biol. Biochem., 35: 1693-1703.
Ferris, M. J. and D. M. Ward. 1997. Seasonal distiributions of dominant 16S rRNA-drfined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis. Appl. Environ. Microbiol., 63: 1375-381.
Fierer, N., J. P. Schimel., P. A. Holden. 2003. Variiations in microbial community composition through two soil depth profiles. Soil Biol. Biochem., 35: 167-176.
Fortun, A., C. Fortun, and C. Ortega. 1989. Effect of farmyard manure and its humic farctions on the aggregate sability of a sandy loam soil. J. Soil Sci., 40: 293-298
Frances, R. L. 1991. Chemical reactions of metal with humic material. Environ. Poll, 70: 185-208.
Friedel, J. K., J. C. Munch, and W. R. Fishcher. 1996. Soil microbial properties and the assessment of available soil organic matter in a haplic luvisol after several years of different cultivation and crop rotation. Soil Biol. Biochem., 28: 479-488.
Frostegard, A., A. Tunlid, and E. Baah. 1991. Microbial biomass measured as total lipid phosphate in soils of different organic content. J. Microbial. Methods, 14: 151-163.
Frostegard, A., E. Baah, and A. Tunlid. 1993. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis. Soil Biol. Biochem., 25: 723-730.
Frostegard, A. and E. Baah. 1996. The use of phospholipid fatty acid analysis to estimated bacteria and fungal biomass in soil Biol. Fertil. Soils, 22: 59-65.
Garcia C., M. Hernandez, F. Costa, and B. Ceccanti. 1994. Biochemical, parameters in soil regenerated by addition of organic wastes. Waste Manag. Res., 12: 457–466.
Garcia C., T. Hernandez, J. Albadalejo, V. Castillo, and A. Roldan. 1998. Revegetation in Semiarid zones: influence of terracing and organic frfuse on microbial activity. Soil Sci. Soc. Am. J., 62: 670-676.
Garland, J. L. and A. L. Mills. 1991. Classification and characterization of heterophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization. Appl. Environ. Microbial., 57: 2351-2359.
Griffith, D. R., E. J. Kladivko, J. V. Mannering, T. D. West, and S. D. Parsons. 1988. Long-term tillage and rotation effects on corn growth and yield on high and low organic matter, poorly drained soil. Agron. J., 80: 599-605.
Hansen, J. W., R S Yost, and N. V. Hue. 1988. Influence of decomposing organic material on soil solution aluminum activity and phytosicity. Agron. Abstr., 1988; 198-198.
Haynes, R J. 1986. The decomposition process: Mineralization, immobilization, humus formation, and degration. pp.52-109. In Haynes (eds.) Mineral Nitrogen in the Plant-Soil System. Academic Press, New York, USA.
He, O. B. and B. R. Singh. 1963. Effects of organic matter on the distribution, extractablity and uptake of Cd in soils. J. Soil Sci., 44: 641-650.
Helyar, K. K. and W. M. Proter. 1986. Soil acidification its measurement and the process involved. pp. 61-101. In Roboson A. D. (ed.) Soil Acidity and Plant Growth. Academic Press, New York, USA.
Hodgson, J. F., W L. Lindsay, and J. F. Trierweiler. 1966. Micronutrient cation complexing in soil solution. II Complexing of znic and copper indisplacing solution from calcareous soil. Soil Sci. Soc Am. Proc., 30: 723-726.
Hortwitz, W. 1980 Official Methods of Analysis of the Association of Offcial Analytical Chemists. pp. 181-192. In Horitiz (ed.) Association of Offcial Analytical chemists, New York, USA.
Hue, N V. 1991. Utilization of nitrogen by vegetable crops. Jpn. Agr. Res. Q., 25: 117-114.
Ingham, E. R., and D. C. Coleman. 1984. Effects of Streptomycin, cycloheximide, fungizone, captan, carbofuran, cygon, and PCNB on soil microorganisms. Microb. Eco., 10: 345-358.
Jokinen, R. 1981. Effect of liming on the magnesium status of some mineral soils and on the fate of fertilizer magnesium. J. Sci. Agr. Soc. Finl., 53: 126-137.
Jones, J. B. Jr. 1990. Plant Nutrition Mannual. pp. 56-57. CRC Press, New York. USA.
Jordan, D., R. J. Kremer, W. A. Bergfield, K. Y. Kim, and N. N. Cacnio. 1995. Evalution of microbial methods as potential indicators of soil quality in historical agricultural fields. Biol Fertil. Soils, 19: 297-302
Jordan, D., R. J. Binnerup, and J. Sorensen. 1996 Potential rates of ammonium oxidation, nitrite oxidation, nitrate reduction and denitrification in the young barley rhizosphere, Soil Biol. Biochem., 28: 47-54.
Kabata-Pendias, A, and H. Pendias. 1984. pp332-336. In ”Trace Elements in Soils and Plants” CRC Press, Inc., Boca Raton, Florida, USA.
Kandeler, E. and H. Gerner. 1988 Short-term assay of soil urease using chlorimetric determination of ammonium. Biol. Fertil. Soils, 8: 199-202.
Kaneda, T. 1991. Iso-and anteiso-fatty acids in bacteria : biosynthesis, function, and taxonomic significance. Microbiol. Mol. Biol. Rev., 55:288-302.
Kaur, A., A Choidhary, and R Kaushik. 2005. Phospholipid fatty acid – a bioindicator of environment monitoring and assessment in soil ecosystem. Curr. Sci., 89: 1103-1112
Kemmitt, S. J., D. Wright, K. W. T. Goulding, and D. L. Jones. 2006. pH regulation of the carbon and nitrogen dynamics in two agricultural soils. Soil Biol. Biochem., 38: 898-911.
Keeney, D. R., and D. W. Nelson. 1982. Modified Griess-Ilosvay method. Pp. 684-697. In Page A. L. (ed.) Methods of Soil Analysis Part 2 Chemical and Microbiological Properties 2nd (ed.) Academic Press, New York, USA.
Khan, S. U. 1970. Enzymatic activity in a gray wooded soil as influenced by cropping systems and fertilizers. Soil Biol. Biochem., 2: 137-139.Klose, S. and and M. A. Tabatabai. 1999. Urease activity of microbial biomass in soil. Soil Biol. Biochem., 31: 205-211.
Klose, S., and M. A. Tabatabai. 1999. Urease activity of microbial biomass in soil. Soil Biol. Biochem., 31: 205-211.
Konopka, A, L. Oliver, and R. F. Turco Jr. 1998. The use of carbon substrate utilization patterns in environmental and ecology microbiology, Microb. Ecol., 35: 103-115.
Kospell, D. E. and D.A. Kospell. 2007. Copper. pp. 316-320. In A. V. Barker and D.J. Pibleam (eds.) Handbook of Plant Nutrition. Taylor and Francis, New York, USA.
Knivett V. A. and J. Cullen. 1965. Some factors affecting cyclopropane acid formation in E. coli. Biochem. J., 96: 771-776.
Ladd, J. N. and J. H. A. Bulter. 1972. Short-term assays of soil proteolytic enzyme activities using proteins and dieptide derivatives as substrates. Soil Biol. Biolchem., 4: 19-30.
Landi, L., L. Badalucco, F Pomare, and P. Nannipieri. 1993. Effectiveness antibiotics to distinguish the contributions of fungi and bacteria to net nitrogen mineralization, nitrification and respiration. Soil Biol. Biochem., 12:1771-1778.
Lioussanne, L., Beauregard, M., Hamel, and C. Jolicoeur.2007. Interaction between arbuscular mycorrhiza and soil microorganisms. In Khasa, and D., Piche. (eds.) Advacne in Mycorhizal Biotechnology Candian Perspective. NRC. Press. Ottawa. USA.
Loll M. J, J.M. Bollag. 1983. Protein transformation in soil. Adv. Agron., 36: 351-382.
Lundquist, E. J., K. M. Scow, L. E. Jackson, S. L. Uesugi, C. R. Johnson. 1999. Rapid response of soil microbial communities from conventional, low input, and organic farming systems to a wet/dry cycle. Soil Biol. Biochem., 31: 1661–1675.
Lupwayi, N. Z., W. A. Rice, and G.W. Clayton. 1998. Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation. Soil Biol. Biochem., 30:1733-1741.
Lynch, J. M. 1990. The Rhizosphere. Wiley, New York. USA.
Marchesini, A., JL. Allievi E. Coomoto, and A. Ferrari. 1988. Long-term effects of quality-compost on soil. Plant Soil, 106: 251-253.
Marinari, S., G. Masciandaro., B. Ceccanti, and S. Grego. 2000. Influence of organic and mineral fertilizer on soil biological and physical properties. Biores. Technol., 71: 9-17.
Martens, D. A., J. Johanson, and J. W. T. Frankenberger. 1992. Production and persistence of soil enzymes with repeated addition of organic residues. Soil Sci., 153: 53-61.
Martyniuk, S. and G. H. Wanger. 1978. Quantative and qualitative examination of microflora associated with different management systems. Soil Sci., 125: 343-350.
Masciandaro G., B. Ceccanti, and C. Garcia. 1994. Anaerobic digestion of straw and piggery waste waters. II. Optimization of the process. Agrochimica, 38: 195-203.
McBride, M. B. 1981. Formes and distribution of copper in solid and solution phase of soil. pp. 24-45. In Loneragan, J. F., R. D. Robson, R. D. Graham (eds.) Copper in Soils and Plants. Academic Press, New York, USA.
McGill, W. B., K. R. Cannon, J. A., Robertson, and E. D. Cook. 1986. Dynamics of
soil microbial biomass and water-soluble organic C in Breton L after 50 years of cropping to two rotations. Can. J. Soil Sci., 66: 1-19.
McLean, E. O. 1982. Soil pH and lime requirement. pp. 199-224. A L. Page (ed.) In Methods of Soil Analysis Part 2 Chemical and Microbiological Properties 2nd . Academic Press, New York, USA.
Meek, B. D., L. E. Grahm, T. J. Donvon, and K. S. Mayberry. 1979. Phosphours availability in a calcareous soil after high loading rates of animal manure. Soil Sci. Soc. Am. J. 43: 741-743.
Mehlich, A. 1985. Melish 3 soil test extractant: extractant: Amodification of Melish 2 extractant. Commun. Soil Sci. Plant Anal., 15: 1409-1416.
Mengel, K. 2007. Potassium. pp. 91-120. In Barker, A. V. and D. J. Pilbeam (eds.) Handbook of Plant Nutrition. Taylor and Francis, Boca Raton, London, UK.
Mills, H. A., and J. B. Jr. Jones. 1979. Nutrient deficiency and toxicities in plants: Nitrogen. J. Plant Nutr., 1: 101-122.
Murphy, J., and J. P. Riley. 1962. A modified single solution method for the determination of phosphate in nature waters. Anal. Chim. Acta, 27: 31-46.
Muyzer, G., E.C. Dewaal, and A. G. Uitterlinden. 1993 Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes ciding for 16S rRNA. Appl. Environ. Microbiol., 59: 695-700.
Muyzer, G, and K. Smalla. 1998. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonic van Leeuwenhoek, 73: 127-141.
Nannipieri, P., S. Greco, B. Ceccanti. 1990. Ecological significance of the biological activity in soil.,. pp. 293–355 In Bollag J. M. and Stotzky, G. (eds.) Soil Biochemistry Vol. 6. Dekker, New York, USA.
Nannipieri, P, E Kanldeler, and P. Ruggiero. 2002. Enzyme activities and microbial and biochemical processes in soil., pp. 1-33. In Burns, R. G., and Dick, R. P. (eds.) Enzymes in the Enviroment: Activity, Ecology and Applications. Dekker, New York, USA.
Nayyar, A., C. Hamel, G. Landford, B. Gossen. K. Hanson, and J. Germida.2009. Soil microbial quality associated with yield reduction in continuous pea. Appl. Soil Ecoil., 43: 115-121.
Nobel, A. D., I. Zenneck, and P. J. Randall. 1996. Leaf litter ash alkalinity and neutralization of soil acidity. Plant Soil, 179: 293-302.
O`Leary, W. M. Wilkinson, S. G., 1988. Gram-positive bacteria. pp. 117-201. In:Ratle, C., Wilkinson, S. G. (ed.), Microbial Lipids. Acedemic Press, London, UK.
Pascual J. A., C. Garcia, and T. Hernandez. 1999. Lasting microbiological and biochemical effects of the addition of municipal solid waste to an arid soil. Biol. Fertil. Soils, 30: 1-6.
Paulson, K. N. and L. T. Kurtz. 1969. Locus of urease activity in soil. Proc. Soil Sci. Soc. Am., 33: 897-901.
Perucci, P. 1992. Enzyme activity and microbial biomass in a field soil amended with municipal refuse. Biol. Fert. Soils, 14: 54–60.
Powlson, D. S. and D. S Jenlinson. 1981. Acomparison of the organic matter, biomass adenosine triphosphate and minerazable nitrogen contents of ploghed and direct-drilled soils. J. Agric., 97: 713-721.
Powlson, D. S., 1994. The soil microbial biomass: before , beyond and back. pp. 3-20. In Ritz, K. J. Dignton, and K. E. Giller. (eds.) Beyond the Biomass-Compositional and Functional Analysis of Soil Microbial Communties. Wiley, Chichester, New York, USA.
Rhoades, J. D. 1982. Soluble salts. pp. 167-168. In Page A. L. (ed.) Methods of Soil Analysis Part2 Chemical and Microbiological Properties 2nd (ed.). Academic Press, New York, USA.
Rhoades, J. and S. Miyamoto. 1990. Testing soils for salinity and sodicity. pp. 299-366. In Westerman R. L. (eds.) Soil Testing and Plant Analysis. Soil Science Society of America, Madison, New York, USA.
Ritz, K., R. E. Wheatley, and B. S. Griffiths. 1997. Effects of animal manure application and crop plants upon size and activity of soil microbial biomas under organically grown spring barley. Biol. Fertil. Soils, 24: 372-377.
Ros, M., M. T. Hernandez, and C. Garcia. 2003. Soil microbial activity after restoration of a semiarid soil by organic amendment. Soil Biol. Biochem., 35: 463-469.
Ros, M., J. A. Pascual, M. T. Hernandez, and C. Garcia. 2006. Hydrolase activities, microbial biomass and bacteria community in a soil after long-term amendment with different composts Soil Biol. Biochem., 38: 3443-3452.
Simek, M. D., W. Hopkins, J. Kalcik, T. Pieck., H. Santruckova, J. Stana, and K. Travnik. 1999. Biological and chemical properties of a arable soils affected by long-term organic and in organic fertilizer applications. Biol. Fertil. Soils, 29: 300-308.
Shuman, L. M. 1991. Chemical forms of micronutrients in soils. pp. 113-144. In J. J. Mortvedt, F. R. Cox, L. M. Shuman and R. M. Welch (eds.) Micronutrients in Agriculture, 2nd ed. Madison, Wisconsin, USA.
Singh, B. B. and J. P. Jones. 1976. Phosphourus sorption and desorption characteristics of soil as affected by organic residue. Soil Sci. Soc. Am. Proc., 40: 389-394.
Skujins, J. 1978. History of abiontic soil enzyme research, pp. 1-49. In R. G. Burns (ed.) Soil Enzymes. Academic Press, New York, USA.
Storey, J. B. 2007. Znic. pp. 422-423. In A. V. Barker and D. J. Pibleam (eds.) Handbook of Plant nutrition. Taylor and Francis, New York, USA.
Tabatabai, M. A. and J. M. Bremner. 1969. Use of p-nitrophenyl phosphate for assay soil phophatase activity. Soil Biol. Biochem., 1: 301-307.
Tabatabai, M. A. and J. M. Bremner. 1970. Arylsulfatase activity of soils. Soil Sci. Soc. Am. Proc., 34: 427-429.
Tabatabai, M. A. and J. M. Bremner. 1972. Arylsulfatase activity of soils. Soil Sci. Soc. Am. Proc., 34: 225-229.
Tabatabai, M. A. 1982. Soil enzymes. pp. 903-947. In Page, A. L. (ed.) Methods of Soil Part 2 Chemical and Microbiological Prospecties. Academic Press, New York, USA.
Tabatabai, M. A. 1994. Soil enzyme . pp. 775-833. In Weaver, R. W. (eds.) Methods of Soil Analysis, Madison, Wisconsin, USA.
Takker, P. N. 1969. Effects of organicmatter on soil iron and manganese. Soil Sci., 108: 108-112.
Thalmann, A. 1968. Zur Methodil der Bestimmuning der DehydorgenaseaktuvitAt im Boden mettels triphenytetrazoliumchlorid (TTC). Landwirtsch. Forsh, 21: 249-258.
Thomas T. D. and R. D. Batt. 1969. Degradation of cell constituents by starved Streptococcus lactis in relation to survival. J., Gen. Microbiol., 58: 347-362.
Trosvilk, V. R. Sorheim, and J. Goksoyr.1996. Total bacterial diversity in soil and sediment communities – a review. J. Ind. MicroBiol. 17: 170-178.
Tunlid, A. and D. C. White. 1992. Biochemical analysis of biomass, community structure, nutritional status, and metabolic activity of microbial communities in soil. pp. 229-262. In Stotzky, G, and J, M. Bollag (eds.) Soil Biochemistry. Marcel Dekker, New York, USA.
Ulrich, B. 1991. An ecosystem approach to soil acidification. pp. 28-79. In Ulrich, B. and M. E. Summer (ed.) Soil Acidity. Springer-Verlag, Berlin, Germany.
Vance, E. D., P. C. Brookes, and D. S. Jenkinson. 1987. An extraction method for measuring soil microbial biomass C. Soil Biol. Biochem., 19: 703-707.
Welbaum, G. E., A. V. Sturz, Z. Zong, and J. Nowal. 2004. Managing soil microorganisms, to improve productivity of agro-ecosytems. Crit. Rev. Plant Sci., 23: 175-193.
Weon, H. Y., J. S. Suh, and W. Y. Choi. 1999. Soil microbial flora and chemical properties as influenced by the application of pig manure compost. Kor. J. Soil Sci. Fertil., 32: 76-83.
Winding, A. 1994. Fingerprinting bacteria soil communities using biolog microtitre plates., pp. 85-94. In Ritz, K. and J. Dighton, K. E. Giller. (eds.) Beyond the Biomass. Wilei Sayce, New York, USA.
Wu J., R. G. Joergensen, B. Pommerening, R. Chaussod, and P. C. Brookes. 1990. Maesurement of soil microbial biomass C by fumigation- extraction –An automated procedure. Soil Biol. Biochem., 22: 1167-1169.
Zak., J. C., M. R. Willing, D. L. Moorhed, and H. G. Wildman. 1994. Functional diversity of microbial communities: a quantitative approach. Soil Biol. Biochem. 26: 1101-1108.