臺灣博碩士論文加值系統

本論文主要探討缺糖對水稻台農67號 (Oryza sativa L. cv. Tainung 67) 懸浮細胞抗氧化防禦系統變化之影響。缺糖明顯抑制懸浮細胞鮮重和乾重，且顯著降低細胞內蛋白質含量與細胞活力。雖然在細胞內無MDA與H2O2增加的現象，但細胞外 (培養基) 發現有大量H2O2累積，而H2O2累積可能會造成氧化逆境的發生。抗氧化酵素中，SOD與CAT活性顯著受缺糖誘導而提升，另外，NADPH oxidase活性也有增加，顯示當NADPH oxidase產生O2·-時，SOD會將其轉換為H2O2，而部分的H2O2會被CAT代謝成水。此外，近年來研究指出H2O2可經由水通道蛋白進出細胞，在水稻懸浮細胞缺糖處理時，亦有發現部分水通道蛋白在缺糖6小時表現量有提升的現象，這些結果說明缺糖會導致懸浮細胞產生氧化逆境，雖然細胞內SOD與CAT活性提升可減緩氧化逆境，但H2O2仍可能藉由水通道蛋白運輸至細胞外，進而造成細胞氧化傷害。

This study focuses on the effect of sugar starvation on the response of antioxidative defense system in rice suspension cells (Tainung 67, Oryza sativa L.). Sugar starvation decreased fresh weight and dry weight of suspension cells, as well as protein content and cell viability. No significant changes of MDA and H2O2 were detected in the cells, however, notable accumulation of H2O2 was found in medium. Activity of SOD and CAT was increased under sugar starvation in suspension cells. In addition, activity of NADPH oxidase was increased indicating that O2·- generated by NADPH oxidase would be converted into H2O2 by SOD, and levels of H2O2 would be converted into H2O by CAT in the cells. It has been known that H2O2 could get in or out of cells via aquaporin (AQP). Under the treatment of sugar starvation, content of some AQP increased after 6 hours. These results proved that sugar starvation would lead to a decrease of activity in both protein content and cell viability of suspension cell from rice. Besides sugar starvation would lead to oxidative stress, H2O2 could be transferred out of the cell via AQP and resulted in oxidative damage to cells, though increase in activity of SOD and CAT can relief oxidative stress.

中文摘要 --------------------------------------------------------------- i
英文摘要 --------------------------------------------------------------- ii
目錄 --------------------------------------------------------------------- iii
圖表目錄 --------------------------------------------------------------- iv
附錄 --------------------------------------------------------------------- v
縮寫字對照表 --------------------------------------------------------- vi
壹、緒言 --------------------------------------------------------------- 1
貳、前人研究 --------------------------------------------------------- 3
參、材料方法 --------------------------------------------------------- 14
肆、結果 --------------------------------------------------------------- 26
伍、討論 --------------------------------------------------------------- 42
陸、參考文獻 --------------------------------------------------------- 52

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