臺灣博碩士論文加值系統

血清素（serotonin）和睡眠的調節有密切的關係。位於腦幹的縫核（raphe nuclei）是主要的血清素神經元所在，若將該系統毀壞，將會產生嚴重失眠（insomnia），包括大幅抑制非速眼動睡眠（non-rapid eye movement sleep），以及幾乎完全抑制逆理睡眠（paradoxical sleep）。光線是一個強而有力影響生物週期與睡-醒狀態的因子。在短亮-暗週期（short light-dark cycle）的光照運作下，光暗會誘發大白鼠的逆理睡眠，光亮則抑制逆理睡眠。血清素抑制劑para-chlorophenylalanine（PCPA）會在貓和大鼠身上導致失眠現象。本研究即藉由施打PCPA抑制血清素系統，觀察其對短亮-暗光照誘發大白鼠睡眠行為之影響，以探討血清素系統介入短亮-暗週期影響逆理睡眠的機制。施打PCPA後，發現逆理睡眠被抑制達50％，而非速眼動睡眠亦下降20％。待若干天大白鼠的睡眠水準恢復到正常，施予短亮-暗週期的光照。結果發現在短亮-暗週期下，PCPA注射組、生理食鹽水注射組均產生顯著的光暗誘發逆理睡眠、光亮抑制逆理睡眠的效果。故短亮-暗週期對睡眠的誘發效果，並不因為抑制血清素而有所差異。接受PCPA注射十二天後測量鼠腦的血清素，並未顯著不同於生理食鹽水注射組；但其代謝物5-hydroxyindoleacetic acid 顯著比生理食鹽水注射組低。因此推測進行短亮-暗週期操弄當時，行為上睡眠恢復和生理上血清素濃度尚低的解離現象仍然存在。故本研究傾向支持一個負向結果，亦即血清素系統並非控制短亮-暗週期光暗誘發逆理睡眠的神經傳導素系統。

Serotonin is actively involved in sleep regulation. Lesions in the raphe nuclei, where the most serotoninergic cells of the brain are located, result in a total loss in paradoxical sleep（PS）and a great reduction in non-rapid-eye-movement（NREM）sleep. Light is a powerful modulator of both biological rhythm and sleep-waking state. Increases and decreases in PS in the dark and light period, respectively, have been observed in albino rats exposed to short light-dark cycles（LD）. Para-chlorophenylalanine（ PCPA）, an inhibitor of serotonin synthesis, induces insomnia in cats and rats. This study aims to determine whether the inhibition of the serotoninegic system induced by PCPA injections alters the effect of LD on PS. PCPA acutely reduced PS by 50% and NREM sleep by 20%. When both PS and NREM sleep subsequently returned to the baseline level, rats were subjected to the LD treatment. Both PCPA- and saline- treated rats significantly showed increases and decreases in PS in the short dark and short light periods, respectively. 5-hydroxyindoleacetic acid, a metabolite of serotonin, was lower in PCPA-treated rats than in saline-treated rats in five of the seven assayed brain regions. It is concluded that the inhibition of the serotoninergic system does not alter the LD effect on PS.

英文摘要
摘要
壹、導論 ………………………………………… 1
貳、材料與方法 ………………………………………… 6
參、結果 ………………………………………… 12
肆、綜合討論 ………………………………………… 29
伍、參考文獻 ………………………………………… 33
附錄 ………………………………………… 39

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