臺灣博碩士論文加值系統

本研究以樣品中的固醇及三酸甘油酯做為生物指標(biomarker)，研發快速簡便的檢驗方法，以快速分別素食加工食品中是否含有動物性成分。本方法以皂化萃取法萃取固醇待測物後，氰甲烷╱甲醇＝60╱40比例，使用流速0.2mL/min之動相沖提2.1mm內徑×50mm長、填充物粒徑3mm的C18管柱進行分離，並以質譜儀進行同位素稀釋法定量，可於一小時內測定樣品中的膽固醇濃度，檢量線之線性範圍介於0.5~50mg/L，應用於實際樣品分析，測得一標示不實的素食樣品。方法二以溶劑萃取法直接萃出樣品中的三酸甘油酯後，使用相同的管柱，流速0.2mL/min，用氰甲烷╱丙酮＝20╱80比例之動相，沖提三酸甘油酯待測物，可於半小時內測得樣品中三酸甘油酯的ECN(equivalent carbon number)分佈；再以ECN的分佈作為分類依據，使用主成份分析分類樣品，判定樣品摻雜動物性油脂的可能性。

第一章 緒論 1
1. 研究動機與目的 1
2. 素食簡介 1
2.1 素食定義 1
2.2 素食與健康 2
2.3 黑心素食 3
2.4 現行檢驗方法 3
3. 油脂簡介 4
4. 文獻回顧 5
5. 參考文獻 6
第二章 以固醇分辨葷素食 11
1. 前言 11
1.1 基本性質 11
1.2 來源 12
1.2.1 動物性來源 12
1.2.2動脈粥狀硬化(Atherosclerosis) 12
1.2.3 植物性來源 13
2. 分析方法簡介 14
2.1 液相層析質譜儀簡介 14
2.2 皂化萃取法 17
2.3 同位素稀釋法 17
3. 研究方法 20
3.1 試藥 20
3.2 樣品 20
3.3 實驗設備 21
3.4 實驗步驟 21
4. 結果與討論 24
4.1 皂化萃取參數－最佳化 24
4.2 同位素稀釋法 25
4.3 層析條件之選擇 27
4.4質譜儀分析膽固醇界面參數之探討 28
4.5 回收率探討 32
5. 結論 34
6. 參考資料 35
第三章 以三酸甘油酯分辨葷素食 58
1. 前言 58
1.1 基本性質 58
1.2 ECN (equivalence carbon number) 58
1.3 PCA(principal component analysis，主成分分析) 59
2. 實驗方法 62
2.1 試藥 62
2.2 樣品 62
2.3 實驗設備 64
2.4 實驗方法 64
3. 結果與討論 67
3.1 萃取條件之選擇 67
3.2層析條件之選擇 67
3.3 三酸甘油酯標準品之分析 68
3.4 真實樣品之分析 70
3.5 真實樣品的PCA分析結果 70
4. 結論 71
5. 參考文獻 72

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