ABSTRACT I
摘要 I
TABLE OF CONTENTS II
LIST OF TABLES IV
LIST OF FIGURES V
CHAPTER ONE INTRODUCTION 1
1.1 Background. 1
1.2 Structure of articular cartilage. 2
1.3 Osteochondral repair and regenerative techniques. 5
1.4 Chondrogenesis and chondrocyte differentiation. 6
1.5 Cartilage tissue engineering. 7
1.5.1 Scaffold properties. 8
1.5.2 Stimulation – CPM. 8
1.4 Literature review. 9
1.5 Previous study 12
1.6 Motivation. 13
1.6 Purpose. 13
CHAPTER TWO MATERIALS AND METHODS 15
2.1 Flow Chart. 15
2.2 Materials 16
2.3 Preparation and mechanical properties of PLGA scaffolds. 17
2.4 Experiment procedures. 18
2.5 Macroscopic evaluation. 21
2.6 Micro-CT scanning. 21
2.7 Histological techniques and scoring system. 22
2.7 Statistics. 25
CHAPTER THREE RESULTS 26
3.1 Rabbit circumstances. 26
3.2 Gross appearance. 27
3.3 Bone formation analysis by micro-CT 31
3.4 Histological examination. 34
CHAPTER FOUR DISCUSSION 43
4.1 PLGA implantation. 43
4.2 Animal study. 43
4.3 Time period. 44
4.4 Cells and histological observation. 45
4.5 Limitations. 46
4.8 Future work. 48
CHAPTER 5 CONCLUSION 49
REFERENCES 51
ACKNOWLEDGEMENTS 56
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