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研究生:陳彥淳
研究生(外文):Yen-Tsun Chen
論文名稱:健康者與心血管病患血液中蛋白質氧化損傷指標之探討
論文名稱(外文):Markers of oxidative damage to proteins in health and cardiovascular diseases
指導教授:趙崇義趙崇義引用關係、鄭美玲鄭美玲引用關係
指導教授(外文):Daniel Tsun-Yee Chiu、Mei-Ling Cheng
學位類別:碩士
校院名稱:長庚大學
系所名稱:基礎醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:2002
畢業學年度:90
語文別:中文
論文頁數:73
中文關鍵詞:氧化損傷、心血管
外文關鍵詞:oxidative damage、3-nitrotyrosine、3-chlorotyrosine
相關次數:
  • 被引用被引用:0
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心血管疾病(cardiovascular disease; CVD)患者被認為體內承受較大的氧化壓力,所以想藉由偵測血漿中蛋白受活性氧攻擊而產生的氧化產物,來做為身體承受氧化壓力大小的指標。本研究之目的是比較一群參與健檢的健康者與CVD患者血漿中的3-chloro-tyrosine(3CT)及3-nitro-tyrosine(3NT)兩種蛋白質氧化指標,方法上是使用HPLC-ECD(ESA)等技術來加以偵測,並以羰基(carbonyl group)作為比較,以了解常人與CVD患者此兩者間氧化壓力的差異。而用HPLC-ECD之方法來測定3CT及3NT乃一新的嘗試,此方法較傳統之ECD detector要敏感約20倍。結果顯示健檢者3CT平均含量為9.5 mol/mol tyrosine,CVD患者含量則為16.6 mol/mol tyrosine;健檢者3NT平均含量為81.2 mol/mol tyrosine,CVD患者含量則為189.0 mol/mol tyrosine。CVD患者其3CT及3NT兩種蛋白氧化指標皆高於健檢者約兩倍,健檢者羰基平均含量為38.2 pmol/mg protein,CVD患者則為50.0 pmol/mg protein;由此可見CVD患者的血液內氧化壓力確實較健檢者為高而導致對周遭蛋白質造成氧化傷害。

目錄 ……………………………………… i
中文摘要 ……………………………………… ii
英文摘要 ……………………………………… iii
序論 ……………………………………… 4
實驗儀器及藥品 ……………………………………… 13
研究方法 ……………………………………… 16
結果 ……………………………………… 19
結論 ……………………………………… 47
參考文獻……………………………………… 69

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