臺灣博碩士論文加值系統

摘要
學號：M9913002
論文名稱：pH值對布氏雙尾藻、日本星桿藻及活動盒型藻生長之影響
總頁數：68頁
學校名稱：國立屏東科技大學 系(所)別：水產養殖系
畢業時間及摘要別：101學年度第1學期碩士學位論文摘要
研究生：鄭雅文 指導教授：翁韶蓮 博士
論文摘要內容：
近年來，麥寮發電廠排煙脫硫系統的低pH值排放水疑似造成海水酸化，對海域生態造成影響。過去數年，浮游藻數量明顯大為降低，而布氏雙尾藻Ditylum brightwellii在某些採樣中仍極其優勢地出現。光度梯度（50 μEm-2s-1、100 μEm-2s-1、150 μEm-2s-1、200 μEm-2s-1）的生長實驗中，結果顯示布氏雙尾藻D. brightwellii在100 μEm-2s-1之光度下生長最佳，其細胞數可達273693±4704 cells/mL，成長率為0.25±0.002 day-1。在溫度梯度(15℃、20℃、25℃、30℃、32℃）的生長實驗中，結果顯示生長細胞數在20℃為最佳，而後隨溫度的增加而細胞數隨之下降。pH值最適生長範圍方面，布氏雙尾藻D. brightwellii細胞數在pH7.0達到最高，其細胞數量15天內可達191880±2213 cells/mL，成長率為0.15±0.01 day-1。另外比較沿岸優勢種，活動盒型藻Biddulphia mobiliensis與日本星桿藻Asterionella japonica，其兩者均在pH7.5 之環境下成長最佳。進一步以混合培養來觀察布氏雙尾藻D. brightwellii與活動盒型藻B. mobiliensis及布氏雙尾藻D. brightwellii與日本星桿藻A. japonica在pH值7.5環境的生長情形及相對優勢。在混合培養實驗中，布氏雙尾藻D. brightwellii均比活動盒型藻B. mobiliensis與日本星桿藻A. japonica的生長快速，且藻族群數量高於另兩種藻，並在種間競爭中有明顯的優勢。因此，推測野外麥寮發電廠排煙脫硫系統之排放水造成鄰近海域pH偏低是造成布氏雙尾藻D. brightwellii大量繁殖主要原因。
關鍵字：pH、浮游藻、布氏雙尾藻、種間競爭

Abstract
Student ID：M9913002
Title of Thesis：Investigation of pH effects on the growth of Ditylum brightwellii, Asterionella japonica, and Biddulphia mobiliensis
Total Page：68
Name of Institute：Department of Aquaculture, National Pingtung University of Science and Technology
Graduate Date：January, 2013 Degree conferred：Master
Name of Student：Ya-Wen Cheng Adviser：Saou-Lien Wong
The Contents of Abstract in This Thesis:
In recent years, Mailiao Power Plant flue gas desulphurization system discharged low pH seawater resulted in acidification impact on marine ecology. Over the past few years, phytoplankton abundance clearly greatly reduced, however Ditylum brightwellii seemed to dominate in some sampling periods. The photometric gradient（50 μEm-2s-1、100 μEm-2s-1、150 μEm-2s-1、200 μEm-2s-1）growth experiments, results show that both of the optimum growth and cell number was highest at 100 μEm-2s-1, the cell number up to 273693±4704 cells/mL, the growth rate was 0.25±0.002 day-1 . Temperatures gradient (15℃、20℃、25℃、30℃、32℃）growth experiments, results show that both of the optimum growth and cell number was best at 20℃, but the number of cells decreased while the temperatures was increased. The optimum pH of D. brightwellii cells for growth was around pH7.0, and also reaching the highest number of growth cells, on day 15, up to 191880±2213 cells/mL, the growth rate was 0.15±0.01 day-1. In addition to comparing the coastal dominant species, Biddulphia mobiliensis and Asterionella japonica was also determined and the pH range for the best growth was around pH7.5. Mixed culture further observes D. brightwellii and B. mobiliensis, D. brightwellii and A. japonica growth situation and the relative advantages of different pH environment. In mixed culture experiments, D. brightwellii than the rapid growth of B. mobiliensis and A. japonica, algal populations higher than the other two algae, showing a distinct advantage in interspecific competition. Therefore, the speculate wild Mailiao power plant flue gas desulfurization system discharge water adjacent waters with low pH cause D. brightwellii blooms main factors.
Key words: pH, phytoplankton, Ditylum brightwellii, interspecific competition

目錄
摘要 I
Abstract III
謝誌 V
目錄 VI
表目錄 VIII
圖目錄 IX
第一章 前言 1
1.1 初級生產者的種類 1
1.2浮游藻之特性 1
1.2.1 浮游藻分類 1
1.2.2 依浮游藻個體大小 2
1.2.3 浮游藻生長週期 2
1.3 浮游藻扮演的角色與重要性 4
1.3.1 魚介類的天然食糧 4
1.3.2 水污染指標 4
1.4環境因子對浮游藻生長之影響 5
1.4.1 氮源之利用 5
1.4.2碳源之利用 6
1.4.3 水溫 6
1.4.4 光度 7
1.4.5 鹽度 7
1.4.6 pH值 8
1.5 雲林離島工業區與沿岸生態 8
1.6 台灣以往西海岸水文因子與浮游藻組成之情況 12
第二章 材料與方法 14
2.1 實驗室培養 14
2.1.1 浮游藻取得來源 14
2.1.2實驗藻種介紹 15
2.2 浮游藻之培養條件 18
2.3環境因子對浮游藻成長濃度之分析 20
2.3.1布氏雙尾藻D. brightwellii最適成長光度實驗 20
2.3.2布氏雙尾藻D. brightwellii最適成長溫度實驗 20
2.3.3 pH值對三種浮游藻成長試驗 21
2.3.4競爭培養實驗 21
2.4 浮游藻生長濃度之分析 23
2.4.1 細胞計數法 23
2.4.2 成長率之計算 23
2.5 統計分析 24
第三章 結果與討論 25
3.1光度與溫度對布氏雙尾藻D. brightwellii成長影響 25
3.1.1布氏雙尾藻D. Brightwellii最適成長光度 25
3.1.2布氏雙尾藻D. brightwellii最適成長溫度 30
3.2 pH值對三種浮游藻成長之影響 35
布氏雙尾藻D. brightwellii 35
活動盒型藻B. mobiliensis 37
日本星桿藻A. japonica 37
3.3兩種浮游藻生長種間競爭影響 50
第四章 結論 60
第五章 參考文獻 61

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