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Purposes: We use a new Ganoderma membrane, which is made from
extracted wasteof fruiting body derived from Ganoderma tsugae,
to evaluate the effect of as awound dressing. Chemical
analysis revealed the Ganoderma membrane is acopolymer of
glucose(60%) and N-acetyl-D-glucosamine(40%). Animals:
FemaleWistar rats weighing 300-400g were used. After
anesthetized with ketamine andpentobarbital by intraperitoneal
injection, dorsal skin hairs were removedwith electric
clipper. Two wounds as mirror-image were made on the back
bydissecting 2*2 cm2 skins in full thickness. Ganoderma
membrane was applied onone wound randomly and gauze was used on
the other. These animals were cagedindividually with neck
collars, and fed regular rat chow and water ad libitum.The wound
areas were measured on the 4th, 8th, 12th, 16th, 20th day
afterthese procedures. Histological examinations of wounds
were also performed onthe 4th, 7th, 16th day postoperatively.
Implantation of these materials wasdone to reveal the
interaction of tissue and dressing. These area data
wereanalyzed with paired Student''s T test. Results: The
Ganoderma wound areas weresignificant smaller than gauze''s.
Histological observation shows Ganodermamembrane induces mild
inflammation and attracts polymorphonuclear leukocytes''
aggregation around the margin. These are many macrophages
and giant cellsinfiltrating gauze (foreign body reaction).
Conclusion: The healing ofGanoderma wound is more smooth than
gauze''s. It costs to perform further teststo evaluate the
possible clinical application due to the enhanced
woundhealing effect of Ganoderma membrane.
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