臺灣博碩士論文加值系統

Lung cancer has been the first cause of death for a long time in Taiwan. Smoking is considered as the primary carcinogen, but the smoking rate of female is even lower in Taiwan. Epidemiology research shows that cooking oil fume is the significant risk factor of lung cancer.
Restaurant workers are study subjects of this research. There were 46 waiters, 59 cooks and 20 vendors recruited to join this study. Urine samples of pre-work and post-work were collected. We use tt-DDE (trans, trans-2,4-decadienal) to represent the exposure of cooking oil fume. Cooks and Vendors used personal air sampling equipment to collect the tt-DDE while they were working. We wanted to develop an LC-MS/MS analysis method for simultaneously quantification of urinary 8-OHdG and cotinine. To monitor the oxidative stress after the exposure of cooking oil fume.
The simultaneously analysis method for quantification of urinary 8-OHdG and Cotinine has been established. Quantification range of cotinine is from 0.5ng/mL to 1000 ng/mL. The limit of detection (LOD) is 0.5ng/mL. Accuracy is from -0.22% to 3.76%. Precision is from 0.86% to 7.67%.
The quantification range of 8-OHdG is from 0.5ng/mL to 15ng/mL. the limit of detection (LOD) is 0.5ng/mL. Accuracy is from -2.28% to 0.19%. Precision is from 1.42% to 8.24%. R2 values are larger than 0.995.
Urinary 8-OHdG concentrations of cooks and vendors are higher than waiters (p-value <0.0001) and urinary 8-OHdG concentrations of post-work are higher than of pre-work. Urinary cotinine concentrations of cooks and vendors are also higher than waiters.( p-value<0.0001)

中文摘要 vi
Abstract： vii
第一章、目的 1
第二章、背景說明 2
2.1肺癌與環境因子 2
2.2 tt-DDE (trans,trans-2,4-Decadienal),反式-2,4-癸二烯醛 3
2.3 8-OHdG 4
2.4 Cotinine 6
2.5 樣品前處理方法 7
2.6分析及儀器原理 9
第三章、材料與方法開發 11
3.1 研究對象 11
3.2藥品與試劑 11
3.3儀器設備 13
3.4移動相配製 13
3.5線上固相萃取(On-Line Solid Phase Extraction)系統 14
3.6方法開發 16
3.7 統計分析 17
第四章 結果與討論 18
4.1 線上固相萃取系統方法開發 18
4.2 方法驗證 20
4.3 統計結果分析 22
4.4 結論與建議 23
參考文獻 52
附錄一、參與研究同意書 54
附錄二、問卷 55

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