臺灣博碩士論文加值系統

在先前實驗室所發展的動態光罩快速成型系統中，使用PCL-PEG-PCL生醫材料混合光交聯劑PEG-HEMA，將其溶於氯仿內，並且成功的製作出支架。然而PEG-HEMA在氯仿內的溶解效果是需要改善的，除此之外舊系統無法製作出多於五層的多孔性支架，因此在此篇研究中，PEG-HEMA的合成與系統的改善，並且製作出層厚較厚且較精準的3D支架。
在PEG-HEMA的合成中，使用98%的HEMA來取代96%的HEMA，此PEG-HEMA不僅僅是在氯仿下可以完全的溶解並且也在其他的溶劑有相當好的溶解效果，例如丙酮，因此PEG-HEMA 以及 PCL-PEG-PCL二次溶解的問題也將一併被改善。此外，系統的架構被改善得更加穩定。以DLP數位光學鏡頭投射器直接取代動態光罩產生器，因此新的系統能有效改善光罩圖形及成型效果。
在3D多孔性支架製作方面，所達到的層數與結果都已較舊系統的佳，但由於前一層已固化的支架所造成光反射的現象，導致支架有過度固化以及在堆疊六層後支架孔洞產生封閉的情況。為此使用三種方法來嘗試解決這個問題—減少每層的固化時間、利用遞減支架來補償支架放大的影響以及使用組合式支架的方式。從結果來看，組合式支架的製作方式能夠縮小支架尺寸誤差，以六層支架來說，其誤差可從40%縮小到5.6%。利用組合式的方法，可能製作出多層且精確的3D支架，並且沒有孔洞封閉的問題。

In the previous Dynamic Mask Rapid Prototyping System developed in our laboratory, PCL-PEG-PCL was mixed with PEG-HEMA in chloroform and cured to create scaffolds successfully. However, the solubility of PEG-HEMA in chloroform is still an issue to be improved. Besides, the old system could not create scaffolds more than 5 layers without pore closure. Therefore, in this research, PEG-HEMA synthesis and the system were improved. Moreover, thicker and more precise 3D scaffolds were fabricated.
In PEG-HEMA synthesis, 98% HEMA was used to replace 96% HEMA. The new PEG-HEMA can be dissolved completely in not only chloroform but also other solvents, such as acetone. The re-dissolution issue of PEG-HEAM and cured PCL-PEG-PCL was also improved. Besides, the structure of the system was redesigned to provide stability and a DLP projector was adopted directly to replace the assembled dynamic mask generator. The new system was capable of providing better mask images and performance.
In 3D porous scaffold fabrication, the achievable layers and results were better than the old system, but due to the reflection of previous cured layers, over-cure happened and pores may be closed after 6 layers. Three approaches were tested to solve this problem—decreasing the curing time of later layers, decreasing the scaffold dimensions of later layers for compensation, and assembling basic scaffold sets. As a result, assembling basic scaffold sets was the best among three and could reduce the scaffold dimension error effectively from 40% to 5.6% for a 6-layer scaffold. With this assembly strategy, it is possible to fabricated thick and precise 3D scaffolds without pose closure problem.

摘要……………………………………………………………………I
Abstract…………………..........................................................................II
致謝…………………………………………………………………….IV
目錄……………………………………………………………………V
圖目錄………………………………………………………………VIII
表目錄…………………………………………………………………XII
第一章 緒論……………………..…………………………………...….1
1.1 前言……………………………………………………………1
1.2 研究背景與動機………………………………………………..2
1.3 研究目的與方法………………………………………………..3
1.4 論文撰寫架構…………………………………………………5
第二章 文獻探討………………………………………………………..6
2.1 組織工程介紹…………………………………………………6
2.1.1支架材料之特性…………………………………………8
2.1.2高分子生醫材料…………………………………………9
2.1.3傳統支架製作技術…………………………………….16
2.2 快速原型技術…………………………………………………19
2.2.1快速原型加工原理………………………………………19
2.2.2利用快速原型技術做組織工程支架……………………20
第三章 材料合成改善與性質分析…………………………………...30
3.1 實驗藥品與設備………………………………………………30
3.1.1實驗藥品…………………………………………………30
3.1.2實驗設備簡介……………………………………………31
3.2 生醫材料合成與改善…………………………………………32
3.2.1 PCL-PEG-PCL 共聚物合成…………………………..32
3.2.2 PEG-HEMA光交聯劑合成……………………………34
3.3 材料核磁共振光譜分析………………………………………38
3.4 材料性質測試…………………………………………………48
3.4.1 材料溶解性質評估……………………………………48
3.4.2 二次溶解測試…………………………………………..49
第四章 系統簡介與機構改善…………………………………………52
4.1 生醫動態光罩快速成型系統…………………………………53
4.1.1先前系統架構……………………………………………53
4.1.2動態光罩產生器…………………………………………54
4.1.3動態光罩控制軟體…………………………………….57
4.2 生醫用動態光罩快速原型系統變更設計……………………59
4.2.1動態光罩產生器問題解決………………………………59
4.2.2機構的設計變更……………………………………….60
4.2.3光學鏡組配置……………………………………………62
4.2.4其他細部變更……………………………………………65
4.3 新系統相關檢測…………………………………………….67
4.3.1床台穩定度測試……………………………………….67
4.3.2系統成像測試……………………………………………67
第五章 3D多層支架製作與檢測……………………………………71
5.1多層支架設計與單層支架參數設立…………………………71
5.1.1多層支架設計……………………………………………71
5.1.2單層支架最佳曝光時間…………………………………73
5.1.3 單層支架孔洞與條寬的量測…………………………74
5.2 3D多孔性支架製作…………………………………………78
5.3 3D多層支架參數設定………………………………………80
5.3.1多層支架每層最佳成型時間………………………….80
5.3.2 遞減支架設計與製作…………………………………..84
5.3.3 組合式多層支架製作…………………………………88
第六章 結論與未來工作………………………………………………94
6.1結論……………………………………………………………94
6.2未來工作………………………………………………………..95
參考文獻………………………………………………………………97

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