臺灣博碩士論文加值系統

本研究以離子凝膠法製備幾丁聚醣(chitosan)奈米粒子，且表面披覆二磷酸腺? (ADP)及纖維蛋白原(Fibrinogen)，探討幾丁聚醣奈米粒子對血液凝固的影響，並了解其血塊形成的的特性。
由實驗結果顯示，製作出之幾丁聚醣奈米粒子粒徑大小在於240~330nm之間，且在凝血實驗方面，血液中加入0.05wt%的奈米粒子，可使血液凝固的時間明顯縮短，凝血時間相較於的未添加奈米粒子的血液NP為63.63±3.09%、ANPx0.25為55.25±2.09%、ANPx1.00為48.28±6.21%、FNP為63.19±4.69%。
血塊質地分析結果得知添加奈米粒子會使得血塊(clot)的硬度(hardness)降低，反之其黏附性(adhesiveness)會增加，並由掃描式電子顯微鏡(SEM)發現，添加奈米粒子的血塊，其血液凝固形成的纖維蛋白(fibrin)較未放粒子的血塊中短且少。
因此，本研究提供一個可以促進血液凝固的方式，未來應可有效應用於生醫材料領域，達到快速止血的目的。

The experiment prepares chitosan nanoparticles by ionic gelation method, and later uses ADP and Fibrinogen respectively to cover the surface of the chitosan nanoparticles to create another two types of nanoparticles, ANP and FNP. The thesis is to explore the effects of chitosan nanoparticles on the formations of blood clots and to understand the properties of clots formation.
According to the experimental results, the particle size of the chitosan nanoparticles is among 240~330nm. Also, in coagulation experiments the clotting time decreases obviously when adding 0.05wt% nanoparticles into blood. Compared to the blood without nanoparticles, the data of experiment group is as followed: NP is 63.63±3.09%, ANPx0.25 is 55.25±2.09%, ANPx1.00 is 48.28±6.21%, and FNP is 63.19±4.69%.
According to the texture profile analysis, adding nanoparticles will decrease the hardness of clots but increase its adhesiveness on the other hand. Furthermore, observation from Scanning Electron Microscopy (SEM) shows that fibrin formed by coagulation in the clots with nanoparticles is shorter and less than which in the clots without nanoparticles.
Consequently, the research provides a method to improve coagulation. This method should be able to be effectively applied to biomaterial field to reach the goal of fast hemostasis in the future.

目錄
中文摘要 i
英文摘要 ii
誌謝 iv
目錄 v
表目錄 vii
圖目錄 viii
第一章、緒論 1
§ 1-1 血液的組成 1
§ 1-2 止血生理學 3
§ 1-2-1 初步止血(Primary Hemostasis) 3
§ 1-2-2 繼發止血(Secondary Hemostasis) 7
§ 1-3 凝血之基本理論 8
§ 1-4 幾丁聚醣 (chitosan) 10
§ 1-5 二磷酸腺? (ADP)及纖維蛋白原 (Fibrinogen) 12
§ 1-6 石英晶體微天平 (Quartz crystal microbalance, QCM) 14
§ 1-6-1 石英晶體微天平( QCM )簡介 14
§ 1-6-2 石英晶體微天平( QCM )感測原理 15
§ 1-7 血液動力黏度參數 19
§ 1-7-1 黏度 19
§ 1-7-2 影響血液黏度的因素 19
§ 1-8質地分析 (Texture profile analysis) 21
§ 1-9 研究動機與目的 23
第二章、實驗設備與方法 24
§ 2-1 實驗藥品 24
§ 2-2 實驗儀器 25
§ 2-3 實驗流程架構 26
§ 2-4 材料製備與分析 27
§ 2-4-1 奈米粒子製作 27
§ 2-4-2 奈米粒子之特性分析 29
§ 2-5 生醫凝血應用測試 34
§ 2-5-1 QCM量測 35
§ 2-5-2 動態錄影血液凝固測量 36
§ 2-5-3凝血過程黏度測試 37
§ 2-5-4血塊力學測試 38
§ 2-5-5血塊形態鑑定 39
第三章、結果與討論 40
§ 3-1 奈米粒子特性分析 41
§ 3-1-1 粒徑分析(Particle size)及界面電位(Zeta potential) 41
§ 3-1-2 傅立葉轉換紅外線光譜儀(FTIR)檢測 42
§ 3-1-3 穿透式電子顯微鏡(TEM)觀察 44
§ 3-2 生醫凝血應用測試 46
§ 3-2-1 QCM量測 46
§ 3-2-2 凝血過程黏度測試 52
§ 3-2-3 血塊力學測試 55
§ 3-2-4 血塊掃描式電子顯微鏡(SEM)觀察 58
第四章、結論 61
參考文獻 62
附錄(一) 65
附錄(二) 66
附錄(三) 67

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