臺灣博碩士論文加值系統

本研究之目的在探討重量訓練後補充黑大蒜以評估對血液中肌肉損傷指標：肌酸激酶 (creatine kinase, CK)、乳酸脫氫酵素(lactic dehydrogenase, LDH) 以及體內有發炎時，指數會迅速上升的高敏感C-反應蛋白 (high sensitivity C-reactive protein, hs-CRP)之影響是否具有效果。方法：以16位高中男生為受試者，完成5項重量訓練動作8-RM、3回合後，隨機抽8人為實驗組，於訓練後0小時立即、24、48小時後共三次黑大蒜補充，8人為控制組不補充。訓練前和之後恢復期24、48、72小時抽血，檢測得CK、LDH、hs-CRP參數後，以成對和獨立樣本t考驗分析兩組組內和組間各時間點差異情形。結果：控制組：CK -運動前與運動後24、48、72小時的差異達顯著水準；LDH -運動後24小時與運動後72小時的差異達顯著水準；hs-CRP-運動後各時間點之間與運動前差異未達顯著水準。實驗組：CK-運動後24小時與運動後48小時的差異達顯著水準；LDH、hs-CRP運動後各時間點之間與運動前的差異未達顯著水準。控制組與實驗組：CK、LDH、hs-CRP 在運動前及運動後各時間點的組間差異未達顯著水準。結論：本研究使用的黑大蒜在補充二次(65ml/次) 後，在運動後48小時是具有可以降低肌肉損傷發炎指標LDH和hs-CRP活性的潛在效用，更可以有效降低CK的活性反應情形。本研究以重量訓練後進行黑大蒜連續補充的方式，提供了不同的研究方式和高強度運動後對身體修護的另一種選擇。
關鍵詞：肌酸激酶、乳酸脫氫酵素、高敏感C-反應蛋白

The study looked at the benefits of black garlic as post-exercise supplementation by examining the variation in the concentrations of creatine kinase (CK), lactic dehydrogenase (LDH) and high sensitivity C-reactive protein (hs-CRP). Methodology: 16 chosen high school male students were randomly assigned to a control group and an experimental group after weight training. Subjects in the experimental group take in black garlic immediately, 24 hrs and 48 hrs after weight training, but this procedure was not applied in the control group. Blood tests were conducted on each subject prior to and after weight training. Statistical analyses were performed afterward. Findings: in the control group, a marked variation was noticed in the concentration of CK. In terms of LDH concentration, the comparison of post 24 hrs results with post 72 hrs results was statistically significant. As for hs-CRP concentration, the comparisons among the results of each post-exercise phase showed no significant differences. The experimental group showed significant decreases in the CK concentration 24 hours and 48 hours after workout. As for the concentration of LDH and hs-CRP, no significant statistical differences were found. To conclude, black garlic supplementation may help lower the concentration of CK, LDH, and hs-CRP after it is implemented twice and serve as a desirable alternative for post-exercise recovery.
Key Words: Creatine Kinase, Lactic Dehydrogenase, High Sensitivity C-reactive Protein

摘要 iii
Abstract iv
誌謝 v
目錄 vi
表目錄 viii
圖目錄 ix
第一章 緒論 1
第一節 研究背景 1
第二節 研究目的 4
第二章 文獻探討 5
第一節 阻力訓練與氧化性壓力 5
第二節 運動訓練所造成的氧化性傷害與發炎反應 6
第三節 肌肉損傷與阻力訓練 9
第四節 肌肉損傷與抗氧化食品增補 10
第五節 黑大蒜的成份與藥理作用 11
第六節 黑大蒜補充 13
第七節 研究之助益 15
第三章 研究方法 16
第一節 研究對象 16
第二節 實驗之設計與流程 16
第三節 測驗時間與地點 22
第四節 血液採集及分析 22
第五節 資料處理與分析 23
第四章 結果與討論 24
第一節 受試者基本資料 24
第二節 運動測試前及運動後血液肌肉損傷指標 26
第三節 討論 35
第五章 結論與建議 44
第一節 結論 44
第二節 建議 45
參考文獻 47
一、中文部分 47
二、英文部分 49
附錄一 受試者志願參與暨家長知情同意書 56

表目錄

表2-1 受試者受驗前黑大蒜補充劑量與時間 13
表4-1 受試者控制組&實驗組基本資料 25
表4-2 受試者控制組&實驗組基本資料平均數統計分析結果 26
表4-3 控制組CK運動前、後平均數統計分析結果 27
表4-4 控制組LDH運動前、後平均數統計分析結果 28
表4-5 控制組hs-CRP運動前、後平均數統計分析結果 29
表4-6 實驗組CK運動前、後平均數統計分析結果 30
表4-7 實驗組LDH運動前、後平均數統計分析結果 31
表4-8 實驗組hs-CRP運動前、後平均數統計分析結果 32
表4-9 控制組與實驗組CK運動前、後平均數統計分析結果 33
表4-10 控制組與實驗組LDH運動前、後平均數統計分析結果34
表4-11 控制組與實驗組hs-CRP運動前、後平均數統計分析結果35

圖目錄

圖2-1 S-丙烯基半胱氨酸 (SAC) 分子結構式 12
圖2-2 黑蒜精產品 15
圖3-1 重量訓練仰臥推舉使用器材及操作示意 17
圖3-2 重量訓練原地負重深蹲使用器材及操作示意 17
圖3-3 重量訓練仰臥起坐負重使用器材及操作示意 18
圖3-4 重量訓練立姿二頭肌負重彎舉使用器材及操作示意 18
圖3-5 重量訓練俯臥弓身負重使用器材及操作示意 19
圖3-6 實驗前準備期流程 20
圖3-7 實驗流程架構 21
圖3-8 Beckman Coulter生化分析儀 AU-5800 23
圖4-1 控制組運動前後各時間點CK活性反應情形 36
圖4-2 控制組運動前後各時間點LDH活性反應情形 37
圖4-3 控制組運動前後各時間點hs-CRP活性反應情形 38
圖4-4 實驗組運動前後各時間點CK活性反應情形 39
圖4-5 實驗組運動前後各時間點LDH活性反應情形 40
圖4-6 實驗組運動前後各時間點hs-CRP活性反應情形 41
圖4-7 控制組與實驗組運動前後各時間點CK活性反應情形 42
圖4-8 控制組與實驗組運動前、後各時間點LDH活性反應情形 42
圖4-9 控制組與實驗組運動前、後時間點hs-CRP活性反應情形 43

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