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研究生:江亭萱
研究生(外文):Ting-Hsuan Chiang
論文名稱:蓋斑鬥魚精巢結構:光學及電子顯微鏡之研究
論文名稱(外文):Light and Electron Microscopic Study on the Testis of Paradise Fish, Macropodus opercularis
指導教授:楊西苑楊西苑引用關係
指導教授(外文):Hsi-Yuan Yang
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:動物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:2000
畢業學年度:88
語文別:中文
論文頁數:1
中文關鍵詞:蓋斑鬥魚精巢結構精子生成
外文關鍵詞:Macropodus opercularistestismorphologyspermatogenesis
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本研究利用組織化學染色、超薄切片,以及透過Tanaka 所開發出來的Osmium-DMSO-Osmium (O-D-O) 冷凍斷裂技術等方法處理樣品,並在光學顯微鏡及電子顯微鏡下,觀察描述蓋斑鬥魚成熟精巢之形態結構。根據實驗觀察結果,蓋斑鬥魚雄魚精巢為細長條狀,呈略帶透明的乳白色。內部由許多管狀構造(細精管)所組成,成樹枝狀排列。由於細精管中無次序散佈許多不同發育階段生殖細胞的包囊,因此判定蓋斑鬥魚精巢為小葉型(lobule type)。精子成熟的過程(spermatogenesis)可粗分為兩個階段:一為精細胞的發育(spermatocytogenesis),二為精子的變態(spermiogenesis)。蓋斑鬥魚精細胞的發育是由初、次級精原細胞(spermatogonia)經有絲分裂形成精母細胞(spermatocyte),而後再經減數分裂形成精細胞(spermatid),過程中細胞型態的變化包含細胞逐漸縮小,以及細胞質內胞器組成及結構上的改變。分裂完成後形成的精細胞再經過精子變態的過程,最後生成具有活力的成熟精子。關於精子變態的過程,依據精細胞中心粒所在的區域、鞭毛的生成、中心粒與細胞核間的相關位置及細胞質的脫落可區分成五個時期;第一期:早期精細胞。中心粒遠離細胞膜而與其它胞器共同存在於細胞質中。第二期:鞭毛形成期。中心粒向細胞膜移動並接觸,而後進行鞭毛生長。第三期:中心粒-細胞核複合體形成期。中心粒與細胞核相接觸乃至於陷入細胞核底部凹陷。第四期:細胞質的丟棄。精細胞細胞質中出現大量空泡,且Sertoli cell細胞質突起包圍精細胞,用以輔助其細胞質丟棄的進行。第五期:精子期。精子頭部為卵圓形,無頂體(acrosome),細胞核內染色質相當緻密聚集,尾部鞭毛為典型9+2微管構造。另外,精巢間質組織(interstitial tissue)當中也存在著許多不同的細胞,其細胞類型包括有myoid boundary cells、萊氏細胞(Leydig cells)等,各細胞擁有不同的形態結構,則分別扮演著不同的生理機能。
摘要 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - i
目次 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - iii
前言 1
1. 緒論 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1
2. 精巢之外觀 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 2
3. 精巢之組織構造 - - - - - - - - - - - - - - - - - - - - - - - - - 3
4. 精子生成 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 4
5. Sertoli cell - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 9
6. 間質組織區細胞 - - - - - - - - - - - - - - - - - - - - - - - - 10
7. 蓋斑鬥魚簡介 - - - - - - - - - - - - - - - - - - - - - - - - - - 11
材料與方法 12
1. 光學顯微鏡樣品製備及組織化學染色 - - - - - - - - - - 12
2. 穿透式電子顯微鏡之樣品製備 - - - - - - - - - -- - - - - - 13
3. 掃描式電子顯微鏡之樣品製備 - - - - - - - - - -- - - - - - 13
4. 生殖細胞之大小測量 - - - - - - - - - - - - - - - - - - - - - 14
結果 15
1. 蓋斑鬥魚雄性生殖腺之外觀 - - - - - - - - - -- - - - - - - 15
2. 精巢之組織結構 - - - - - - - - - - - - - - - - - - - - - - - - - 15
3. 精子生成 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 17
4. 間質組織區細胞結構 - - - - - - - - - - - - - - - - - - - - - - 25
討論 27
1. 精巢的構造 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 27
2. 精細胞的發育 - - - - - - - - - - - - - - - - - - - - - - - - - - 27
3. 精子變態 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 32
參考文獻 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 40
圖表及說明 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 49
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