臺灣博碩士論文加值系統

金屬和其錯合物無論在生物化學或環境化學中，皆扮演著不可或缺的角色
；而金屬分析的工作也就日趨重要。近年來高效能液相層析法，配合各種
檢測器已經成為分析及定量金屬的利器之一。本研究乃利用 4-（2-
pyridylazo）resorcinol（PAR）為錯合劑，分別和銅及鈷形成錯合物之
後再以逆相高效能液相層析儀配合紫外－可見光之偵測器進行銅及鈷之分
析工作。同時探討銅和鈷與 PAR 形成錯化合物之光譜性質和層析分析時
之最佳條件，並進一步探討分析程序之靈敏度及偵測極限。經實驗結果顯
示：靜相為 RP-C▋▋column，而動相組成為Acetonitrile/methanol/
tartrate buffer（pH6.5）＝6/30/64含1.0×10▋▋ M 離子對試劑
(tetrabutylammonium bromide）時為最佳分離條件。此時銅的滯留時間
為 9.23 分鐘，鈷的滯留時間為15.94分鐘。本研究再進一步將此方法應
用於分析超合金標準品211W11221D，並評估其再現性和準確度。

Since metals and their complexes play an important role in both
biological chemistry and in enviromental chemistry, the
analysis of metals is rather important. In recent years, high
performance liquid chromatography coupled with various
detectors has become one of the best methods for metal analysis.
In this study, we used 4-（2-pyridylazo）resorcinol (PAR） as a
pre-column reagent, Cu-PAR and Co-PAR were separated and
quantitatively determined by reverse phase high performance
liquid chromatography with UV-VIS detector. We also
investigated the absorption and chromatographic characteristics
of these metal chelates. The optimum condition established in
this work was:acetonitrile/methanol /tartrate buffer (pH=6.5)=
6/30/64 including 1.0×10▋▋ ion-pair reagent(
tetrabutylammonium bromide) The separation was done with C▋▋
eversed phase column. The proposed method was appilied to
determine Cu and Co in a relatively complex substance, namely,
nickel-base superalloy. The precision and accuracy of the
proposed chromatographic method were evaluated。